Multiplex PCR based genotypic characterization of pathogenic vancomycin resistant Enterococcus faecalis recovered from an Indian river along a city landscape

Lata, Pushpa; Ram, Shri; Shanker, Rishi

doi:10.1186/s40064-016-2870-5

Cited by 19 publications

(20 citation statements)

References 37 publications

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“…The measured mean concentrations (log cfu/100 mL) of E. coli (2.9-3.4) and enterococci (3.5-4.0) are comparable to other studies conducted in developing countries, for instance, in China (E. coli 1.8-3.4) (Liu et al 2009), India (enterococci 2.18-5.84) (Lata et al 2016), Southeast Asia (E. coli 2.8-4.3) (Widmer et al 2013), and Côte d'Ivoire (E. coli 2.55-3.47) (Adingra et al 2012). In the present study, 88% of E. coli and all enterococci samples exceeded bathing water quality standards (specified by USEPA), which indicates potential health risks associated with the use of the river water for domestic, bathing and irrigation purposes.…”

Section: Discussionsupporting

confidence: 86%

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The Impact of Environmental Variables on Faecal Indicator Bacteria in the Betna River Basin, Bangladesh

2017

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Environmental variables influence Faecal Indicator Bacteria (FIB) in surface water. Understanding that influence is important, because presence of FIB, which are an indication of faecal contamination, means that harmful pathogens could be present that could also be influenced by environmental variables. Although some recent studies have focused on this topic, most of this work has been conducted in developed countries. Similar studies in developing countries and in a (sub)tropical climate are lacking. In this study we assess the influence of environmental variables on fluctuations in FIB concentrations of the Betna River in southwest Bangladesh that floods almost every year. Monthly water samples from five locations along Betna River were tested for FIB (E. coli and enterococci) in 2014-2015. A linear regression model was developed to assess the effect of the environmental variables on FIB concentrations. The study revealed increased FIB concentrations during wet weather conditions. Precipitation and water temperature were positively correlated with FIB concentrations. Water temperature was positively correlated, because the warm May to September period coincides with frequent precipitation. Precipitation increases manure release from land to surface water. The regression model explains nearly half of the variability in FIB concentrations (R 2 of 0.46 for E. coli and 0.48 for enterococci). This study indicates that increased precipitation combined with higher water temperature, as is expected in this region with climate change, likely increases FIB concentrations. Waterborne pathogens are expected to respond similarly to these environmental changes, indicating that disease outbreaks could well become more frequent and severe.

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Section: Discussionsupporting

confidence: 86%

“…The water quality of surface waters has been deteriorating in developing countries due to rapid industrialization and population growth (Lata et al 2016). The Betna River in southwest Bangladesh is a surface water source for about 0.1 million people living along the river.…”

Section: Introductionmentioning

confidence: 99%

The Impact of Environmental Variables on Faecal Indicator Bacteria in the Betna River Basin, Bangladesh

2017

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“…The measured mean concentrations (log cfu/100ml) of E. coli (2.9-3.4) and enterococci (3.5-4.0) are comparable to other studies conducted in developing countries, for instance, in China (E. coli 1.8-3.4) (Liu et al, 2009), India (enterococci 2.18-5.84) (Lata et al, 2016), Southeast Asia (E. coli 2.8-4.3) (Widmer et al, 2013), and Côte d'Ivoire (E. coli 2.55-3.47) (Adingra et al, 2012). In the present study, 88% of E. coli and all enterococci samples exceeded bathing water quality standards (specified by USEPA).…”

Section: Discussionsupporting

confidence: 86%

“…The measured mean concentrations of E. coli (2.9-3.4 log cfu/100ml) and enterococci (3.5-4.0 log cfu/100ml) is comparable to few studies (Liu et al, 2009, Adingra et al, 2012, and slightly lower than few other studies (Lata et al, 2016, Widmer et al, 2013 conducted in developing countries. The measured concentrations are probably relatively low as a consequence of the tidal in and outflowing water and consequent low residence time of the bacteria.…”

Section: Spatiotemporal Variability Of Fib and Impact Of Environmentasupporting

confidence: 80%

Assessing the impact of socio-economic development and climate change on faecal indicator bacteria in the Betna River, Bangladesh

Islam¹

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“…Therefore, the present paper showed that the vanA gene was positive in 2 isolates (25%) of E. faecium and 9 isolates (50%) of E. faecalis by HRMA technique, also, primer specificity for vanA gene was 100.01% and melting temperature for this gene observed at 79.9 ± 0.5°C. Lata et al [21] reported a real-time PCR assay for detection of VRE with a sensitivity of 10 3 genomic equivalents. Cha et al [22] reported a multiplex real-time PCR assay Fig.…”

Section: Discussionmentioning

confidence: 99%

Development of high-resolution melting curve analysis in rapid detection of vanA gene, Enterococcus faecalis, and Enterococcus faecium from clinical isolates

et al. 2020

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Background: High-resolution melting analysis (HRMA) is a novel molecular technique based on the real-time PCR that can be used to detect vancomycin resistance Enterococcus (VRE). The purpose of this study was to identify VRE species with HRMA in clinical isolates.Results: Out of 49 Enterococcus isolates, 11 (22.44%) E. faecium isolates and 19 (38.77%) E. faecalis isolates were detected. Average melting temperatures for divIVA in E.faecalis, alanine racemase in E.faecium, and vanA in VRE strains were obtained as 79.9 ± 0.5°C, 85.4 ± 0.5°C, and 82.99 ± 0.5°C, respectively. Furthermore, the data showed that the HRMA method was sensitive to detect 10 0 CFU/ml for the divIVA, alanine racemase, and vanA genes. Also, out of 49 Enterococcus spp., which were isolated by HRMA assay, 8 isolates (16.32%) of E. faecium and 18 isolates (36.73%) of E. faecalis were detected. The vanA gene was reported in 2 isolates (25%) of E. faecium and 9 isolates (50%) of E. faecalis. Conclusions: This study demonstrated that using the HRMA method, we can detect E. faecium, E. faecalis, and the vanA gene with high sensitivity and specificity.

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Multiplex PCR based genotypic characterization of pathogenic vancomycin resistant Enterococcus faecalis recovered from an Indian river along a city landscape

Cited by 19 publications

References 37 publications

The Impact of Environmental Variables on Faecal Indicator Bacteria in the Betna River Basin, Bangladesh

The Impact of Environmental Variables on Faecal Indicator Bacteria in the Betna River Basin, Bangladesh

Assessing the impact of socio-economic development and climate change on faecal indicator bacteria in the Betna River, Bangladesh

Development of high-resolution melting curve analysis in rapid detection of vanA gene, Enterococcus faecalis, and Enterococcus faecium from clinical isolates

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