Multiplex size marker (YEAST PLEX) for rapid and accurate identification of pathogenic yeasts

Abstract

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A case of Candida metapsilosis conjunctivitis in a neonate admitted to the cardiac heart intensive care unit

“…18 The incidence of fungemia caused by C. orthopsilosis and C. metapsilosis has been increasingly reported in recent years. [19][20][21] C. metapsilosis is a rare entity in nosocomial Candida infections. Previous studies adjusted the frequency of infections attributed to C. metapsilosis from 0% to 35.5% of all C. parapsilosis sensu lato around the world.…”

“…orthopsilosis and C. metapsilosis has been increasingly reported in recent years. 19 , 20 , 21 C . metapsilosis is a rare entity in nosocomial Candida infections.…”

“…The incidence of fungemia caused by C . orthopsilosis and C. metapsilosis has been increasingly reported in recent years 19‐21 . C .…”

Multiple Candida strains causing oral infection in COVID-19 patients under corticosteroids and antibiotic therapy: An observational study

“…A two-step multiplex polymerase chain reaction assay named YEAST PLEX that identifies 17 clinically important common to uncommon yeasts (Candida albicans, Candida dubliniensis, Candida parapsilosis, Candida auris, Candida glabrata, Candida kefyr, Candida krusei, Candida tropicalis, Candida guilliermondii, Candida rugosa, Candida intermedia, Candida lusitaniae, Candida norvegensis, Cryptococcus neoformans, Rhodotorula mucilaginosa, Trichosporon spp. and Saccharomyces cerevisiae) was used according to the instructions as previously described (Aboutalebian et al, 2022). It is notable that the specificity of YEAST PLEX was tested using several reference strains belonging to 17 species and DNA samples of clinically significant non-target bacteria, parasites, fungi and human genomic DNA.…”

“…It is notable that the specificity of YEAST PLEX was tested using several reference strains belonging to 17 species and DNA samples of clinically significant non-target bacteria, parasites, fungi and human genomic DNA. Moreover, the YEAST PLEX method has the ability to identify mixed yeast colonies (Aboutalebian et al, 2022).Sequencing of internal transcribed spacer (ITS) rDNA using primers ITS5 and ITS4 was conducted for strains that weren't identified by YEAST PLEX multiplex PCR.…”