Multiplexed Determination of Amino‐Terminal Pro‐B‐Type Natriuretic Peptide and C‐Reactive Protein Cardiac Biomarkers in Human Serum at a Disposable Electrochemical Magnetoimmunosensor

Ávila, Berta Esteban‐Fernández de; Escamilla‐Gómez, Vanessa; Lanzone, Valentina; Campuzano, Susana; Pedrero, Marı́a; Compagnone, Darío; Pingarrón, José M.

doi:10.1002/elan.201300479

Cited by 42 publications

(10 citation statements)

References 16 publications

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“…The comparison between the dual assay and the RR1 and RR2 single assays (Table 1) shows that the current density is higher in the dual assays, but the RR1 to RR2 ratio is maintained at about 0.5, which confirms that there is no cross-talk between the working electrodes due to product diffusion, in line with other dual approaches using TMB or other soluble peroxidase substrates [23][24][25]. It is important to note that the amount of MNPs added to the working electrode is different in dual and single assays, about 8.9 and 18.8 µg, respectively.…”

Section: Analytical Characteristics Of the Dual Magnetogenoassaysupporting

confidence: 67%

Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788

Plácido

Pereira

Barroso

et al. 2019

Sensors and Actuators B: Chemical

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Development of expeditious analytical methods for the detection of genetically modified organisms (GMOs) is increasingly necessary, not only to verify compliance with labelling, but also to help industry to efficiently control the reception of raw materials. On the basis of this, a disposable electrochemical magnetogenoassay is proposed for simultaneous detection of two Roundup Ready (RR) soybean lines GTS 40-3-2 and MON89788, using gold-coated magnetic nanoparticles (Fe3O4@Au) as nanosupport. To perform this magnetogenoassay, a sandwich-type hybridization assay was used with different enzymatic labelling systems (fluorescein isothiocyanate and digoxigenin) and dual screen-printed carbon electrodes (SPdCEs), which allowed the simultaneous readout of each target. A linear relationship ranging from 0.1 to 2.5 nM and from 0.1 to 1.0 nM was achieved for GTS-40-3-2 and MON89788 events, respectively, and both assays showed a similar detection limit of about 0.1 nM. Furthermore, a good performance in terms of precision and selectivity was achieved. The proposed approach is a step forward for event-specific multiplex detection.

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Section: Analytical Characteristics Of the Dual Magnetogenoassaysupporting

confidence: 67%

Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788

Plácido

Pereira

Barroso

et al. 2019

Sensors and Actuators B: Chemical

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“…Both types of biomarkers have significant value in diagnosis and prognosis of cardiac disease [25,70]. In an interesting paper, Esteban Fernández de Ávila et al [26] proposed the first multiplexed configuration for NT-proBNP and CRP consisting of a magnetic microparticles-based immunosensing platform involving carboxylic acid-modified magnetic beads for covalent immobilization of NT-proBNP antigen anti-CRP specific capture antibodies (Figure 3). The quantification of both analytes was performed by indirect competitive and sandwich-type immunoassays, respectively, using HRP-labeled tracers.…”

Section: Multiplexed Electrochemical Immunosensors Involving Electmentioning

confidence: 99%

“…The main analytical characteristics of the methods developed for the simultaneous determination of biomarkers involving electrochemical immunosensors constructed with electrode arrays are given in Table 1 (refs. [7,8,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32]). Since cancer is a major threat of global health, growing demand exists for the development of reliable devices for the detection of this disease, and therefore, most of the proposed designs are devoted to cancer markers.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed Electrochemical Immunosensors for Clinical Biomarkers

Yáñez‐Sedeño

Campuzano

Pingarrón

2017

Sensors

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Management and prognosis of disease requires the accurate determination of specific biomarkers indicative of normal or disease-related biological processes or responses to therapy. Moreover since multiple determinations of biomarkers have demonstrated to provide more accurate information than individual determinations to assist the clinician in prognosis and diagnosis, the detection of several clinical biomarkers by using the same analytical device hold enormous potential for early detection and personalized therapy and will simplify the diagnosis providing more information in less time. In this field, electrochemical immunosensors have demonstrated to offer interesting alternatives against conventional strategies due to their simplicity, fast response, low cost, high sensitivity and compatibility with multiplexed determination, microfabrication technology and decentralized determinations, features which made them very attractive for integration in point-of-care (POC) devices. Therefore, in this review, the relevance and current challenges of multiplexed determination of clinical biomarkers are briefly introduced, and an overview of the electrochemical immunosensing platforms developed so far for this purpose is given in order to demonstrate the great potential of these methodologies. After highlighting the main features of the selected examples, the unsolved challenges and future directions in this field are also briefly discussed.

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“…For their simultaneous determination in human serum, a rapid magnetoimmunosensor was developed [56]. HOOC-MMPs were used to covalently immobilize a specific capture antibody or the target antigen, and the quantification of CRP and NT-proBNP was performed by using sandwich and indirect competitive configurations, respectively, as well as HRP-labeled tracers and amperometric detection (H 2 O 2 /TMB) at SPdCEs.…”

Section: Micromagnetic Particlesmentioning

confidence: 99%

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

Yáñez‐Sedeño

Campuzano

Pingarrón

2016

Sensors

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Ultrasensitive biosensing is currently a growing demand that has led to the development of numerous strategies for signal amplification. In this context, the unique properties of magnetic particles; both of nano- and micro-size dimensions; have proved to be promising materials to be coupled with disposable electrodes for the design of cost-effective electrochemical affinity biosensing platforms. This review addresses, through discussion of selected examples, the way that nano- and micro-magnetic particles (MNPs and MMPs; respectively) have contributed significantly to the development of electrochemical affinity biosensors, including immuno-, DNA, aptamer and other affinity modes. Different aspects such as type of magnetic particles, assay formats, detection techniques, sensitivity, applicability and other relevant characteristics are discussed. Research opportunities and future development trends in this field are also considered.

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Multiplexed Determination of Amino‐Terminal Pro‐B‐Type Natriuretic Peptide and C‐Reactive Protein Cardiac Biomarkers in Human Serum at a Disposable Electrochemical Magnetoimmunosensor

Cited by 42 publications

References 16 publications

Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788

Chronoamperometric magnetogenosensing for simultaneous detection of two Roundup Ready™ soybean lines: GTS 40-3-2 and MON89788

Multiplexed Electrochemical Immunosensors for Clinical Biomarkers

Magnetic Particles Coupled to Disposable Screen Printed Transducers for Electrochemical Biosensing

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