Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray

Wojciechowski, Jason; Danley, David L.; Cooper, John; Yazvenko, Nina; Taitt, Chris R.

doi:10.3390/s100403351

Cited by 25 publications

(18 citation statements)

References 25 publications

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“…28–30 SEB is extremely toxic with an inhalation exposure LD 50 of 20 ng kg −1 and the ability to incapacitate 50% of the population (ED 50 ) with just 0.4 ng kg −1 . 31,32 Multiple highly sensitive immunoassays, including immunosensors, point-of-care assays, 33 and protein microarrays, 34 have been developed for the sensitive detection of SEB. 15 ‘ 35 ‘ 36…”

Section: Introductionmentioning

confidence: 99%

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Jenko

Zhang

Kostenko

et al. 2014

Analyst

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Plant and microbial toxins are considered bioterrorism threat agents because of their extreme toxicity and/or ease of availability. Additionally, some of these toxins are increasingly responsible for accidental food poisonings. The current study utilized an ELISA-based protein antibody microarray for the multiplexed detection of ten biothreat toxins, botulinum neurotoxins (BoNT) A, B, C, D, E, F, ricin, shiga toxins 1 and 2 (Stx), and staphylococcus enterotoxin B (SEB), in buffer and complex biological matrices. The multiplexed assay displayed a sensitivity of 1.3 pg mL(-1) (BoNT/A, BoNT/B, SEB, Stx-1 and Stx-2), 3.3 pg mL(-1) (BoNT/C, BoNT/E, BoNT/F) and 8.2 pg mL(-1) (BoNT/D, ricin). All assays demonstrated high accuracy (75-120 percent recovery) and reproducibility (most coefficients of variation <20%). Quantification curves for the ten toxins were also evaluated in clinical samples (serum, plasma, nasal fluid, saliva, stool, and urine) and environmental samples (apple juice, milk and baby food) with overall minimal matrix effects. The multiplex assays were highly specific, with little cross-reactivity observed between the selected toxin antibodies. The results demonstrate a multiplex microarray that improves current immunoassay sensitivity for biological warfare agents in buffer, clinical, and environmental samples.

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Section: Introductionmentioning

confidence: 99%

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Jenko

Zhang

Kostenko

et al. 2014

Analyst

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“…Staphylococcal enterotoxin B (SEB) has been extensively investigated [59][60][61][62][63][64]. Wojciechowski et al [65] used a microchip sensor with a disposable slide including an organic photodiode for SEB detection, allowing a LOD of 0.5 ng/mL.…”

Section: Bacterial Toxin Sensing Methodsmentioning

confidence: 99%

Biotoxin sensing in food and environment via microchip

Zhang

et al. 2014

Electrophoresis

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Biotoxin contamination in food and environmental samples has threatened health or life of human and animals. Thus, a rapid lab-independent sensing method for biotoxin determination is urgently required. Microchip sensing system allows a promising rapid and low-cost detection strategy. Herein, the recent development of various microchips, including microfluidic chip and microarray, has been discussed to sense various biotoxins in food and environmental samples (i.e. phytotoxin, animal toxin, marine toxin, and mycotoxin). Microchip can be served as both analyte transportation and sensing platform, via either labeling or labeling-free sensing strategy. Because of its fast sensing time, low sample consumption, ready portability, and high compatibility, it has been extensively employed in biotoxin determination in both academic and industrial circle. With the advances of fabrication strategies and sensing modes, the microchip performance has been dramatically improved, including sensitivity, efficiency, reliability, stability, cost saving, portability. The potential applications can be found wide spread in biotoxin sensing in the near future, while their practical application in real sample need to be addressed.

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“…Disposable electrochemical sensors and biosensors have significant applications as diagnostic tools in the point-of-care testing. The use of several disposable biosensors in the point-ofcare assessment of acetylcholinesterase, 127 bacteria, [128][129][130][131] cancer biomarkers, [132][133][134] and DNA targets 135 has been reported in the literature. A portable sensor system comprising sixteen electrodes for DNA detection is illustrated in Figure 9.…”

Section: Point-of-care Diagnosticsmentioning

confidence: 99%

“…Different strategies have been proposed for the detection of cardiac biomarkers under the concept of point-of-care diagnostics. [129][130][131][132][133][134][135] For example, Jagadeesan et al have designed a low-cost and rapid method for the modification of paper electrodes with conducting polymers for the amperometric detection of troponin 138 for point-of-care diagnosis. Polyaniline was deposited on the patterned screen printed paper electrodes by electrodeposition, followed by immobilization of anti-cardiac troponin-I using 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N -hydroxysuccinimide coupling.…”

Section: Point-of-care Diagnosticsmentioning

confidence: 99%

Disposable Biosensors for Clinical Diagnosis

Janegitz¹,

Cancino²,

Zucolotto³

2014

j. nanosci. nanotech.

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We present an overview on the use of disposable electrochemical biosensors for diagnostics, focusing on the applications of these devices as immunosensors and DNA sensors in the point-of-care diagnostics. Analytical biosensors have emerged as efficient alternatives for the detection of innumerous diseases, because of their high specificity and the convenience of detecting the electrochemical signals produced by the presence of an analyte using a portable equipment. This review highlights the recently developed strategies toward immobilization of different biological molecules on disposable electrodes such as carbon nanotubes and metal nanoparticles. In the course of the review, we first introduced the disposable biosensors, followed by an overview of the immunosensors, and discussed the applications of DNA sensors and disposable biosensors in point-of-care diagnostics. We also have evaluated the prospects and future applications of these devices in the field of biomedical research.

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Multiplexed Electrochemical Detection of Yersinia Pestis and Staphylococcal Enterotoxin B using an Antibody Microarray

Cited by 25 publications

References 25 publications

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

Biotoxin sensing in food and environment via microchip

Disposable Biosensors for Clinical Diagnosis

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