Multiplexed Immunoassays in Food Analysis

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

“…Labels are made of coloured or fluorescent nanoparticles with sizes of 15-800 nm, allowing an unobstructed flow through the membrane. They are often made of colloidal gold [1,[17][18][19][20] or latex [21], less often selenium [22], carbon [2,23] or liposomes [24][25][26][27][28][29] are used. Latex nanoparticles are coloured [21] for optical detection.…”

“…Some immunosensor formats are surface plasmon resonance, grating coupler, total internal reflectance fluorometry, magnetobiosensor and interferometer sensor. However, in some publications an LFA with optical transduction is called a "biosensor" [24,26,58,79]. The magnetobiosensor, under development at Philips Research, may be a serious threat upon market introduction, because of its concept of full integration and disposable assay cartridges [104,105].…”

Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

“…Good progress has been made in these efforts, specifically in bacterial assays. Direct bacterial nucleic acid detection has been attempted in lateral flow format (Mao et al, 2009;Lo et al, 2013;Baeumner, 2004) and paper microfluidic format (Fronczek et al, 2014;Costa et al, 2014), and these assays are fairly rapid and show good sensitivity, with the limit of detection being typicallyo10 3 colony forming units (CFU) per mL. However, these formats often require sample pre-treatment, separate nucleic acid extraction, or separate thermocycling, and sometimes lack specificity.…”

Effect of magnetic coupling on the magnetization reversal in arrays of magnetic nanocaps

“…Prominent examples are the creation of high-efficiency hybrid solar cells 1,2 and sensoric applications for pathogen detection. 3,4 Another important field is the potential of magnetic nanostructures as future magnetic recording media. [5][6][7][8][9][10] Current conventional granular storage media are facing the superparamagnetic limit 7,9 and in order to keep future growth in areal density, new recording concepts are required.…”