Multiplexed label-free optical biosensor for medical diagnostics

Bottazzi, Barbara; Fornasari, Lucia; Frangolho, Ana; Giudicatti, Silvia; Mantovani, Alberto; Marabelli, F.; Marchesini, Gerardo; Pellacani, Paola; Therisod, Rita; Valsesia, Andrea

doi:10.1117/1.jbo.19.1.017006

Cited by 54 publications

(60 citation statements)

References 43 publications

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“…The prototype of a new stand-alone, portable SPR-based imaging system has recently been described with proof-of-concept for detection of PTX3 [26]. Our data, with the characterization of the SPR protocols allowing sensitive and reproducible detection of pathological PTX3 levels in human plasma, offers the basis for further improvement of these devices, with very important diagnostic applications.…”

Section: Discussionmentioning

confidence: 93%

A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of Pentraxin-3 in Human Plasma

Canovi

Lucchetti

Stravalaci

et al. 2014

Sensors

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A new immunoassay based on surface plasmon resonance (SPR) for the rapid, reproducible and sensitive determination of pentraxin-3 (PTX3) levels in human plasma has been developed and characterized. The method involves a 3-min flow of plasma over a sensor chip pre-coated with a monoclonal anti-PTX3 antibody (MNB4), followed by a 3-min flow of a polyclonal anti-PTX3 antibody (pAb), required for specific recognition of captured PTX3. The SPR signal generated with this secondary antibody linearly correlates with the plasma PTX3 concentration, in the range of 5–1500 ng/mL, with a lowest limit of detection of 5 ng/mL. The PTX3 concentrations determined with the SPR-based immunoassay in the plasma of 21 patients with sepsis, ranging 15–1600 ng/mL, were superimposable to those found in a classic ELISA immunoassay. Since the PTX3 concentration in the plasma of healthy subjects is <2 ng/mL, but markedly rises in certain medical conditions, the method is useful to quantify pathological levels of this important biomarker, with important diagnostic applications. In comparison with the classic ELISA, the SPR-based approach is much faster (30 min versus 4–5 h) and could be exploited for the development of new cost-effective SPR devices for point-of-care diagnosis.

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Section: Discussionmentioning

confidence: 93%

A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of Pentraxin-3 in Human Plasma

Canovi

Lucchetti

Stravalaci

et al. 2014

Sensors

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“…To this end, we realized and optimized the prototypal device already introduced in the material and method section, more details can be founded in [32].…”

Section: Imaging Capabilitiesmentioning

confidence: 99%

A multiplexed label free plasmonic nano-device for near infrared applications

Floris¹,

Fornasari²,

Frangolho³

et al. 2015

AIP Conference Proceedings

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“…We choose the structure with main plasmonic resonance peak at about 800 nm because of its spectral affinity with some near-IR emitters of practical interest. This plasmonic platform has already shown to work as a sensitive device for medical diagnostics [18].…”

Section: Introductionmentioning

confidence: 99%

Thickness controlled sol-gel silica films for plasmonic bio-sensing devices

Figus

Quochi

Artizzu

et al. 2014

AIP Conference Proceedings

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Plasmonics has recently received considerable interest due to its potentiality in many fields as well as in nanobio-technology applications. In this regard, various strategies are required for modifying the surfaces of plasmonic nanostructures and to control their optical properties in view of interesting application such as bio-sensing, We report a simple method for depositing silica layers of controlled thickness on planar plasmonic structures. Tetraethoxysilane (TEOS) was used as silica precursor. The control of the silica layer thickness was obtained by optimizing the sol-gel method and dip-coating technique, in particular by properly tuning different parameters such as pH, solvent concentration, and withdrawal speed. The resulting films were characterized via atomic force microscopy (AFM), Fourier-transform (FT) spectroscopy, and spectroscopic ellipsometry (SE). Furthermore, by performing the analysis of surface plasmon resonances before and after the coating of the nanostructures, it was observed that the position of the resonance structures could be properly shifted by finely controlling the silica layer thickness. The effect of silica coating was assessed also in view of sensing applications, due to important advantages, such as surface protection of the plasmonic structure

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Multiplexed label-free optical biosensor for medical diagnostics

Cited by 54 publications

References 43 publications

A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of Pentraxin-3 in Human Plasma

A New Surface Plasmon Resonance-Based Immunoassay for Rapid, Reproducible and Sensitive Quantification of Pentraxin-3 in Human Plasma

A multiplexed label free plasmonic nano-device for near infrared applications

Thickness controlled sol-gel silica films for plasmonic bio-sensing devices

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