Multiplexed microsphere-based flow cytometric assays

Size-controlled synthesis, surface functionalization, and biological applications of thiol-organosilica particles

“…Recently, multiplexed flow cytometric assays have become popular as rapid, highly sensitive, and accurate assays for mixtures of multiple samples of small volume in the same tube [22,23]. Small polystyrene and latex microspheres have been used as a support in such assays.…”

Fluorescent organosilica micro- and nanoparticles with controllable size

“…Recent approaches in diagnostic bead-based assays have required the implementation of optical encoding strategies, whereby the uniform incorporation of encoding elements into nano-and microparticles is of major importance [7,16]. Traditional silica microsphere production has utilised the wellknown Stöber synthesis method, as mentioned above.…”

Multiplexed Immunoassays

“…38,39 Each set of microspheres can then be derivatized by a specific type of capture ligand. As shown in Figure 3 for a typical sandwiched immunoassay, binding of target analyte brings reporter molecules to the microsphere surface.…”