Multiplexed near-infrared in vivo imaging complementarily using quantum dots and upconverting NaYF4:Yb3+,Tm3+ nanoparticles

Abstract

A new multiplexed NIR in vivo imaging is showcased by using quantum dots and NaYF(4):Yb(3+),Tm(3+) nanoparticles. The 'temporal' multiplexing is demonstrated by alternating the excitation wavelengths and unmixing the emissions of different probes. Multiplexed cellular imaging and the cellular trafficking in animal models are shown.

Upconversion Nanoparticles: Design, Nanochemistry, and Applications in Theranostics

“…A new multiplexed NIR in vivo imaging has also recently been shown by using NIR QDs and NaYF 4 :Yb 3+ /Tm 3+ nanoparticles. 225 Instead of utilizing the emitting dye to produce additional UC color, the emission bands of UC can also be selectively quenched to produce varied color outputs via utilizing the strong absorption of unremitting dye or nanoparticles. For example, Wolfbeis et al reported tuning of dual UC emission from either the NaYF 4 :Yb 3+ /Er 3+ or the NaYF 4 :Yb 3+ /Tm 3+ nanoparticles for ratiometric optical encoding by introducing various amounts of amino-reactive organic dye on the UCNP surface to selectively quench an intended UC band.…”

Optical properties of amphiphilic copolymer-based self-assemblies

“…To surmount this obstacle, an appealing alternative has emerged for using NIR light based on lanthanide doped upconverting nanoparticles (UCNPs) [119,120]. UCNPs absorb NIR light and convert it to higher-energy photons in the UV, visible, and NIR regions.…”

Recent Advances in Design and Fabrication of Upconversion Nanoparticles and Their Safe Theranostic Applications

“…In the work of Liu group,118 they reported that PEGylated UCNPs physically loaded with organic dyes via hydrophobic force could showed tuned visible emission spectra owing to the FRET from UCNPs to the organic dyes under NIR excitation, and thus could be serve as a FRET nanosystem for in vivo multicolor UCL imaging ( Figure ). Additionally, Kim et al170 have recently demonstrated multiplexed imaging complementarily using QDs and NaYF 4 :Yb/Tm NPs by alternating the excitation wavelengths and unmixing the emissions of different probes.…”