Multiplexed Optical Imaging of Energy Substrates Reveals That Left Ventricular Hypertrophy Is Associated With Brown Adipose Tissue Activation

Abstract

A multiplexed optical imaging technique is presented that allows substrate uptake to be simultaneously quantified in multiple tissues in a high throughput manner. The activation of brown adipose tissue occurs early in the onset of left ventricular hypertrophy, which produces tissue-specific changes in substrate uptake that may play a role in the systemic response to cardiac pressure overload.

Biomedical Imaging in Experimental Models of Cardiovascular Disease

“…The cost and complexity of PET (cyclotron, radiopharmacy) also limit its broader application in mice, and optical techniques, including fluorescence and Cerenkov luminescence, may allow some aspects of myocardial energetics to be assayed with significantly less complexity. 67…”

The Gift of Light

“…In this issue of Circulation: Cardiovascular Imaging, Panagia et al 15 aimed to develop a multiplexed optical imaging platform to simultaneously assess the uptake of glucose and fatty acid analogs in multiple organs in a murine model of LV hypertrophy (LVH). They hoped that this approach would leverage the strength of optical imaging to examine multiple processes in various tissues in a simple and high-throughput fashion.…”

Metabolic Dysfunction in Aortic Stenosis: A Key Piece of the Pathophysiological Puzzle

“…Preliminary observations in small animal models suggest that pressure overload may induce changes in metabolically active tissues outside the heart, such as brown adipose tissue. 7 The metabolic derangement in AS may, therefore, not be isolated to the heart, but human studies will be needed to establish this. We hope that Monga et al, 6 as well as other groups with expertise in the imaging of cardiac energetics, will continue to perform P31-spectroscopy studies.…”