Multiplexed Sensing and Imaging with Colloidal Nano- and Microparticles

Carregal‐Romero, Susana; Caballero-Díaz, Encarnación; Beqa, Lule; Abdelmonem, Abuelmagd M.; Ochs, Markus; Hühn, Dominik; Suau, Bartolome Simonet; Valcárcel, Miguel; Parak, Wolfgang J.

doi:10.1146/annurev-anchem-062012-092621

Cited by 67 publications

(49 citation statements)

References 159 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tunability of QD emission wavelength, large Stokes shifts, and wide absorption spectra enables them to be used as multiplexing agents. [223][224][225] As in the case of Au NPs, QDs can be functionalized with biomolecules, which allow for competitive assays or simply analyte recognition whereby the fluorescence is "switch on/off". 226 Next in this section, basic principles and some examples regarding surface-enhanced Raman scattering (SERS) biosensing are discussed.…”

Section: Figmentioning

confidence: 99%

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Pino¹

2014

J. Biomed. Opt

View full text Add to dashboard Cite

Abstract. Continuous advances in the field of bionanotechnology, particularly in the areas of synthesis and functionalization of colloidal inorganic nanoparticles with novel physicochemical properties, allow the development of innovative and/or enhanced approaches for medical solutions. Many of the present and future applications of bionanotechnology rely on the ability of nanoparticles to efficiently interact with electromagnetic (EM) fields and subsequently to produce a response via scattering or absorption of the interacting field. The crosssections of nanoparticles are typically orders of magnitude larger than organic molecules, which provide the means for manipulating EM fields and, thereby, enable applications in therapy (e.g., photothermal therapy, hyperthermia, drug release, etc.), sensing (e.g., surface plasmon resonance, surface-enhanced Raman, energy transfer, etc.), and imaging (e.g., magnetic resonance, optoacoustic, photothermal, etc.). Herein, an overview of the most relevant parameters and promising applications of EM-active nanoparticles for applications in life science are discussed with a view toward tailoring the interaction of nanoparticles with EM fields. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

Section: Figmentioning

confidence: 99%

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Pino¹

2014

J. Biomed. Opt

View full text Add to dashboard Cite

show abstract

“…Consequently, GNPs can be used for studies in which distances are expected to extend beyond 10 nm or studies in which multiple dyes need to be quenched. It is also likely that a single NP has the ability to quench several dyes of different energies simultaneously, thereby enabling the analysis of several target molecules in a single experiment (multiplexed assays; Figure 5) [56].…”

Section: Gnp-based Caspase Activity Sensorsmentioning

confidence: 99%

“…NPs are eminently suitable for multiplexed assays [56]. Due to their high surface area, they can be functionalized with more than one type of ligand (or recognition element) simultaneously, each being distinguished by a signaling label that is specific for them.…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%

Nanoparticle-based Caspase Sensors

Maysinger

Hutter

2015

Nanomedicine (Lond.)

View full text Add to dashboard Cite

Recent advances in nanotechnology have provided new tools for measuring enzymatic activities that are relevant for the assessment of physiological and pathological processes. Caspases, the enzymes intimately linked with cell death and inflammation, are cysteine-dependent aspartate-directed proteases. The measurement of caspase activity requires assays that can provide data with specificity, precision and sensitivity. Several nanoparticle-based assays are now beginning to emerge. This article will first provide a brief discussion of conventional methods of measuring caspase activity and their limitations, followed by an overview of the advantages and limitations of nanoparticle-based strategies for sensing caspase enzymatic activity in vitro and in vivo.

show abstract

“…[4][5][6][7] For instance, QDs bear sizedependent emission, from UV to mid-IR region, [ 8 ] broad excitation wavelength, large Stokes' shift, symmetric and narrow emission spectra, high brightness, and they do not display photobleaching phenomenon. [ 9 ] As a consequence, QDs provide versatile tools for optical or electrical multiplexing (detection of multiple signals, spectrally, spatially, or temporally resolved), [ 6,[10][11][12] long-term and single molecule imaging, [ 13,14 ] and offer novel approaches in biotechnology, [ 15 ] based on bar-coding, [ 16 ] chemical noses, [ 17 ] logic-gate operations, and optical switches [ 18 ] among others.…”

Section: Introductionmentioning

confidence: 99%

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

Gravagnuolo

Morales‐Narváez

Matos

et al. 2015

Adv Funct Materials

View full text Add to dashboard Cite

Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal\ud protein, is known to self-assemble into chemically stable amphiphilic fi lms,\ud to be able to change wettability of surfaces, and to strongly adsorb other\ud proteins. Herein, a fast, highly homogeneous and effi cient glass functionalization\ud by spontaneous self-assembling of Vmh2 at liquid–solid interfaces is\ud achieved (in 2 min). The Vmh2-coated glass slides are proven to immobilize\ud not only proteins but also nanomaterials such as graphene oxide (GO) and\ud quantum dots (QDs). As models, bovine serum albumin labeled with Alexa\ud 555 fl uorophore, anti-immunoglobulin G antibodies, and cadmium telluride\ud QDs are patterned in a microarray fashion in order to demonstrate functionality,\ud reproducibility, and versatility of the proposed substrate. Additionally, a\ud GO layer is effectively and homogeneously self-assembled onto the studied\ud functionalized surface. This approach offers a quick and simple alternative to\ud immobilize nanomaterials and proteins, which is appealing for new bioanalytical\ud and nanobioenabled applications

show abstract

Multiplexed Sensing and Imaging with Colloidal Nano- and Microparticles

Cited by 67 publications

References 159 publications

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Tailoring the interplay between electromagnetic fields and nanomaterials toward applications in life sciences: a review

Nanoparticle-based Caspase Sensors

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

Contact Info

Product

Resources

About