Multiplexing with SERS labels using mixed SAMs of Raman reporter molecules

Gellner, Magdalena; Kömpe, Karsten; Schlücker, Sebastian

doi:10.1007/s00216-009-2868-8

Cited by 72 publications

(70 citation statements)

References 4 publications

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“…The presence of a close and dense packing of Raman reporter molecules within the SAM on the metal surface minimizes the coadsorption of other molecules from the surrounding onto the metal surface (no spectral interference) and ensures the maximum coverage of the metal surface with Raman reporter molecules (high sensitivity). 18,40 The carboxyl groups in the SAM on the surface of the Au/Ag-NS are activated by EDC/s-NHS for bioconjugation to antibodies. The bioconjugation step was conrmed and optimized by ELISA using an enzyme-labeled antibody (see the Experimental part for details).…”

Section: Characterization Of Antibody-sers Label Conjugatesmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

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Section: Characterization Of Antibody-sers Label Conjugatesmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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“…31,32 DTNB was chosen as a SERS label as it has a high Raman cross-section, high reactivity to gold surfaces, and has been used previously. [33][34][35][36][37] To find the optimal DTNB concentration for functionalizing the nanostars with this label, a range of concentrations (10 mM, 1 mM, 0.1 mM, 0.01 mM, and 0.001 mM) was tested on these nanostars. An optimal concentration of 0.1 mM was chosen since it did not affect the stability of the nanostars.…”

Section: Nanostar Synthesis and Functionalizationmentioning

confidence: 99%

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

D’Hollander¹,

Mathieu²,

Jans³

et al. 2016

IJN

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The need for sensitive imaging techniques to detect tumor cells is an important issue in cancer diagnosis and therapy. Surface-enhanced Raman scattering (SERS), realized by chemisorption of compounds suitable for Raman spectroscopy onto gold nanoparticles, is a new method for detecting a tumor. As a proof of concept, we studied the use of biocompatible gold nanostars as sensitive SERS contrast agents targeting an ovarian cancer cell line (SKOV3). Due to a high intracellular uptake of gold nanostars after 6 hours of exposure, they could be detected and located with SERS. Using these nanostars for passive targeting after systemic injection in a xenograft mouse model, a detectable signal was measured in the tumor and liver in vivo. These signals were confirmed by ex vivo SERS measurements and darkfield microscopy. In this study, we established SERS nanostars as a highly sensitive contrast agent for tumor detection, which opens the potential for their use as a theranostic agent against cancer.

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“…Ranging from metallic nanoparticles labelled with reporter molecules [65][66][67][68][69] to more complicated systems with mixed reporter monolayers [70], dually functionalised systems consisting of reporter molecules and membrane penetrating or targeting antibodies [71][72][73], peptides [65,74,75] or oligomers. To minimise or prohibit degradation from the surrounding environment nanostructures can be afforded a certain degree of stability by silica [13,76] or polymer encapsulation.…”

Section: Surface Enhanced Raman Spectroscopy (Sers)mentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS): Potential applications for disease detection and treatment

McAughtrie

Faulds

Graham

2014

Journal of Photochemistry and Photobiology C: Photochemistry Re

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This version is available at https://strathprints.strath.ac.uk/49189/ Strathprints is designed to allow users to access the research output of the University of Strathclyde. Unless otherwise explicitly stated on the manuscript, Copyright © and Moral Rights for the papers on this site are retained by the individual authors and/or other copyright owners. Please check the manuscript for details of any other licences that may have been applied. You may not engage in further distribution of the material for any profitmaking activities or any commercial gain. You may freely distribute both the url (https://strathprints.strath.ac.uk/) and the content of this paper for research or private study, educational, or not-for-profit purposes without prior permission or charge.Any correspondence concerning this service should be sent to the +44 (0) Highlights: A range of diseases can be detected by Raman and SERS in vitro, ex vivo and in vivo  In vivo multiplexed detection of cancer was achieved  SERS is also a key step in disease treatment  Studies must be further extended to a physiologically relevant medium and in vivo  The potential exists for the application of the techniques in a clinical setting

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Multiplexing with SERS labels using mixed SAMs of Raman reporter molecules

Cited by 72 publications

References 4 publications

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Development of nanostars as a biocompatible tumor contrast agent: toward in vivo SERS imaging

Surface enhanced Raman spectroscopy (SERS): Potential applications for disease detection and treatment

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