Multiplication of HVT FC-126 (Herpesvirus turkey) virus in the kidney cell lines of no avian origin

Abstract

The presented experiments were aimed to cultivate and multiplicate HVT FC-126 in the PLA (Adult pig kidney) and GL-4 (Gerbil kidney) cell lines. Two different HVT FC-126 vaccine strains were used: Marikal SPF (Veterina d.o.o., Croatia) and Lyomarex (Merial, USA). They were adapted to the PLA and GL-4 cell lines. After adaptation, they were titrated on PLA (TCID50 24.23) and GL-4 (TCID50 24.96). On both cell lines they show similar CPE (cytophatic effect). The difference between them was detected using Real Time PCR, which was also positive by agarose gel analysis for the virus contained in Lyomarex, but not in the Marikal SPF. It can be concluded that both cell lines are sensitive to HVT FC-126 and the virus can be multiplied in high titers though much lower than in the calf intestinal epithelial cell line (CIEB) cells (TCID50 27.93)

Functional cell models of the gut and their applications in food microbiology — A review

“…The mechanisms of interactions between foodborne pathogens, mammalian host and intestinal microflora, including mechanisms of microbial attachment and cross-talk with host epithelium, preventive and curative effects of probiotic bacteria still remain obscure, mainly due to restricted experimental systems and in vitro cell models. It has been previously shown that established cell lines provide a relevant in vitro model system to study intestinal pathogen-host cell interactions Filipic et al, 2009Filipic et al, , 2006Ivec et al, 2007;Nissen et al, 2009;Pipenbaher et al, 2009;Schierack et al, 2006). Epithelial cells in pig intestinal cell model were tested for susceptibility to virus infection using rotavirus, transmissible gastroenteritis virus (TGEV) and hepatitis E virus (HEV).…”