Multiplication of tobacco mosaic virus in tobacco callus tissues and in vitro selection for viral disease resistance

Abstract

Tobacco mosaic virus-resistant tobacco was selected in vitro using callus tissues induced from axillary buds of systemically infected tobacco plants. Callus lines in which the virus was continuously multiplying were first isolated and redifferentiated into shoots. By the procedure, non-diseased, healthy shoots were successfully isolated from diseased shoots, which showed typical mosaic symptoms of the virus, and regenerated into intact plants.These regenerated plants showed resistance to virus inoculation, and selfed progeny of virus-resistant regenerants segregated the resistance and susceptibility according to the Mendelian system.

Application of cell and tissue culture and in vitro selection for disease resistance breeding — a review

“…Toyoda et al (1989a) induced callus tissues from axillary buds of tobacco systemically expressing mosaic symptoms of TMV. Callus lines with high amounts of TMV were subcultured for 6 months in order to increase the amount of somaclonal variation and to multiply the variant cells.…”

Evaluation of Stable Resistance Expression in Self-pollinated Progenies of Bacterial Wilt Resistant Regenerants Obtained from Leaf Callus of Tomato.

“…Somaclonal variation in plant tissue cultures is a useful genetic source for crop improvement [1,2] One of the major approaches in plant biotechnology has been an effective isolation and utilization of useful somaclonal variations for producing disease resistant lines in important crop plants [3,4]. Actually, some investigators have successfully isolated somaclonal variants for viral [5,6], bacterial [7,8], and fungal disease resistance [9][10][11] . However, these variations are frequently unstable and therefore, it is essential to evaluate stable propagation of isolated variations in progeny plants.…”

Localization of lily symptomless virus and cucumber mosaic virus in anther- and filament-derived calluses and effect of callus culture duration on virus-free bulblet production in Lilium × ‘Enchantment’

“…It can be concluded that in regenerating virus-free plants, anther-derived nodular callus are superior to anther-derived friable and filament-derived calluses, and an efficient production of virus-free plants from nodular callus in the present study was related to the histological origin and cytological characteristics of the calluses. Several plant viruses remained in callus cultures for a certain period (Toyota et al 1989;Pelah et al 1994;Chen et al 2003), but viruses such as CMV and TMV (Mori and Hosokawa, 1977), TGMV (Slomka et al 1989), CTV (DuranVila et al 1989), and TYLCV (Pelah et al 1994) decline or disappear when the culture duration of callus is extended. The present study also indicated that the virus-free bulblets increased as the culture duration of callus was prolonged (Table 2 and Fig 2).…”