Multiplication végétative in vitro de Gardenia jasminoïdes Ellis

Dumanois, Christine; Godin, Benoit; Bigot, Claude Le

doi:10.1016/s0176-1617(84)80131-5

Cited by 11 publications

(6 citation statements)

References 9 publications

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“…Furthermore, it was difficult to separate them from the explant. The production of shoots with short internodes is a common result of high cytokinin levels (Dumanois et al 1984). In this study, after the initiation of shoots, explants were transferred to fresh SH medium containing auxin (2.9 I~M IAA or 2.5~M IBA) but devoid of BA to minimize the inhibitory effect of the cytokinin on shoot elongation.…”

Section: Shoot Productionmentioning

confidence: 99%

Micropropagation ofPenstemon serrulatus and iridoid formation in regenerated plants

Wysokińska¹

1993

Plant Cell Tiss Organ Cult

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A method for the micropropagation of Penstemon serrulatus Menz. from shoot tips or nodal segments was developed. Multiple microshoot cultures (up to 20 shoots from a single explant) were obtained by maintenance of shoot tip explants on Schenk & Hildebrandt medium (SH) supplemented with 4.4 I~M benzyladenine (BA) or 8.9 IxM BA and 0.57 IxM indole-3-acetic acid (IAA). Microshoots developed into numerous, normal shoots when explants were transferred to SH medium containing 2.9 IxM IAA or 2.5 I~M indole-3-butyric acid (IBA). Shoot cultures were also established from nodal segments (max. 6.8 shoots per segment) when they were placed on SH medium with 0.49 ixM IBA and 2.2 IxM BA. Rooting of shoots was better on SH medium containing auxin (IBA, NAA or IAA) than on SH medium without growth regulators. The plantlets were then transferred to pots and grown in the greenhouse. Four-month-old regenerated plants demonstrated similar iridoid content (leaves contained 3.83% dry wt. penstemide and 1.8% dry wt. serrulatoloside) as the original plants.

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Section: Shoot Productionmentioning

confidence: 99%

Micropropagation ofPenstemon serrulatus and iridoid formation in regenerated plants

Wysokińska¹

1993

Plant Cell Tiss Organ Cult

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“…Although 2iP has been reported as the most efficient cytokinin for several Ericaceae, Norton & Norton (1985) reported that Arctostaphylos uvaursi reacts better to BA. Yield could be slightly increased when 25/,M BA and 20/~M IAA were added together in the culture medium as was shown for Gardenia jasminoides (Dumanois et al 1984).…”

Section: Discussionmentioning

confidence: 58%

In vitro micropropagation of Arctostaphylos uva-ursi (L.) Sprengel.: Comparison between two methodologies

Kada

Dorion

Bigot

1991

Plant Cell Tiss Organ Cult

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“…Mizukami (1983) found that various auxins such as NAA and IAA could be used for induction and subculture of the callus in the presence or absence of cytokinins. Dumanois et al, (1984) reported that white loose callogenesis was derived from leaf explants in the presence of 2,4-D in the culture medium at 0.2 mg/l and 2iP at 0.2 mg/l or BAP 1 mg/l, while brown-white callus tissues with newly formed roots were obtained in the presence of NAA in the culture medium at 1.0 mg/l and KIN at 0.5 mg/l. Gardenia callus tissues grow well on chemically defined media (Ueda et al, 1981).…”

Section: Multiplication Stagementioning

confidence: 99%

“…Salim and Hamza (2017) showed that the maximum mean values of shoots length, shoot number, number of nodes and leaflets number per shoot of G. jasminoides were obtained when MS medium was augmented with combination 3.0 mg/l TDZ + 0.3 mg/l IAA; whereas the concentration 1.0 mg/l IBA; gave highest average of roots number. Callogenesis of gardenia can be induced easily from various parts of the plants (Mizukami et al, 1983;Dumanois et al, 1984) and best callus growth was derived from leaf explants cultured on MS medium. Various auxins such as 2,4-D, NAA, and IAA were tested for induction and subculture of the callus in the presence or absence of cytokinins.…”

Section: Introductionmentioning

confidence: 99%

“…Various auxins such as 2,4-D, NAA, and IAA were tested for induction and subculture of the callus in the presence or absence of cytokinins. Dumanois et al (1984) manifested that white loose callus tissues were formed from leaf explants in the presence of 2,4-D (0.2 mg/l) and 2iP (0.2 mg/l) or BAP (1 mg/l) while brownwhite callus tissues with newly formed roots were obtained in the presence of NAA (1 mg/l) and kin (0.5 mg/l).…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

Gaber

Barakat

2019

Alexandria Science Exchange Journal

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Gardenia jasminoidesEllis is an evergreen plant with fragrant creamy white flowers and glossy, darkgreen leaves, belongs to family Rubiaceae and member of the genus Gardenia. The current study aimed to afford a new technique for in vitro propagation and callus induction of G. jasminoides by using single nodes excised from soft cuttings using full strength MS salts supplemented with 30g/l sucrose, 4g/l gelrite and different concentrations of plant growth regulators. Generally, the current study showed that NAA and BA at 0.0 and 1.0 mg/l, respectively gave rise to the best results for initiation stage. Meanwhile, fortified the basal MS medium with BA and NAA at 2.0 and 0.250 mg/l, consecutively; resulted in the best results with respect to multiplication stage. Regarding rhizogenesis, the neoformed shoots produced from vegetative multiplication stage were rooted successfully upon cultured on MS medium supplemented with 1.0 mg/l IBA and NAA at either 0.250 or 0.500 mg/l, whereas the highest number of roots was recorded besides callus induction. Regarding embryonic callus induction, adding 2,4-D at 1.0 mg/l; enhanced embryonic callogenesis. Neoformed plantlets were acclimatized ex vitro and in vivo vigorously in mixture of perlite and peatmoss at (1:1, v/v), which resulted in the highest percentage value of survival / percent (100%) and successfully flowered and showed true-to-type plants ex vitro.

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Multiplication végétative in vitro de Gardenia jasminoïdes Ellis

Cited by 11 publications

References 9 publications

Micropropagation ofPenstemon serrulatus and iridoid formation in regenerated plants

Micropropagation ofPenstemon serrulatus and iridoid formation in regenerated plants

In vitro micropropagation of Arctostaphylos uva-ursi (L.) Sprengel.: Comparison between two methodologies

Micropropagation and Somatic Embryogenesis Induction of Gardenia jasminoides Plants

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