Multiplicity of Cell Wall Degrading Glycosidic Hydrolases Produced by Apple Infecting <i>Botrytis cinerea</i>

Urbanek, H.; Zalewska-Sobczak, Jadwiga

doi:10.1111/j.1439-0434.1984.tb00757.x

Cited by 22 publications

(7 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We know that Botrytis is capable of secreting several kinds of proteases [17]. However, this fungus is also capable of producing other kinds of enzymes such as pectinases [23], laccase, and a multiplicity of plant cell wall degrading glycosidic hydrolases [35]. In our study, the laccase and pectinases had little/no impact on the yeast macromolecules.…”

Section: Fexpmentioning

confidence: 53%

Impact of Botrytis cinerea Contamination on the Characteristics and Foamability of Yeast Macromolecules Released during the Alcoholic Fermentation of a Model Grape Juice

et al. 2020

View full text Add to dashboard Cite

Botrytis cinerea is a fungal pathogen responsible for the decrease in foamability of sparkling wines. The proteolysis of must proteins originating from botrytized grapes is well known, but far less information is available concerning the effect of grape juice contamination by Botrytis. The impact from Botrytis on the biochemical and physico-chemical characteristics of proteins released from Saccharomyces during alcoholic fermentation remains elusive. To address this lack of knowledge, a model grape juice was inoculated with three enological yeasts with or without the Botrytis culture supernatant. Size exclusion chromatography coupled to multi-angle light scattering (SEC-MALLS) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) techniques (AgNO3 and periodic acid Schiff staining) was used in the study. When Botrytis enzymes were present, a significant degradation of the higher and medium MW molecules released by Saccharomyces was observed during alcoholic fermentation whilst the lower MW fraction increased. For the three yeast strains studied, the results clearly showed a strong decrease in the wine foamability when synthetic musts were inoculated with 5% (v/v) of Botrytis culture due to fungus proteases.

show abstract

Section: Fexpmentioning

confidence: 53%

Impact of Botrytis cinerea Contamination on the Characteristics and Foamability of Yeast Macromolecules Released during the Alcoholic Fermentation of a Model Grape Juice

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, cellulases are also secreted by pathogens during infection, playing a critical role in softening and decomposing plant host cell wall materials to facilitate pathogen infection and host invasion (Boddu et al., ). In one study cellulase was shown to hydrolyse β‐1,4 glycoside bonds within the cellulose polymer and has been reported to increase the virulence of Botrytis cinerea (Urbanek & Zalewska‐Sobczak, ). Here, we identified three cellulase‐related genes (c131347, c131373, and c131434) that exhibited significant expression differences before and after infection of pumpkin seedlings.…”

Section: Discussionmentioning

confidence: 99%

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Zhao

Zhang

et al. 2018

Journal of Phytopathology

View full text Add to dashboard Cite

The parasitic pathogenic fungus Stagonosporopsis cucurbitacearum (Sc.) is the main cause of pumpkin gummy stem blight (GSB), one of the most devastating pumpkin crop diseases in north‐east China. In the present study, transcriptome analyses of the GSB‐susceptible strain commonly grown in that region, zq‐1, were performed to identify differentially expressed genes (DEGs) relevant to GSB infection. Using RNA‐seq of six fungal samples, we obtained 44.62 Gb of clean sequence data with a Q30 base percentage of 92.38% or higher. We carried out functional annotation by comparing sequences against NR, Swiss‐Prot, KEGG, COG, KOG, GO, and Pfam databases and obtained annotation results for 38,110 unigenes. From these unigenes, a total of 341 DEGs were identified and subjected to GO functional annotation and KEGG pathway analysis. Subsequently, 52 genes possessed putative pathogenic functions, with 34 genes assigned to molecular function (including hydrolase, protein kinase, phosphatase, cellulase, etc.), 15 genes assigned to biological process, and 3 genes assigned to mitogen‐activated protein kinase pathways, ABC transport proteins and cellular endocytosis. Identification of DEGs should enhance our understanding of pathogenic genes involved in pumpkin infection by Sc. and should facilitate the systematic investigation of pathogenic gene expression patterns and host–pathogen interactions.

show abstract

“…These include: cutinase, which hydrolyses secondary ester linkages of the cutin polymer (Salinas et al, 1992); cell-walt-degrading enzymes, i.e., pectolytic enzymes (exo-and endo-PG, PL and PME), during the first phase of host-pathogen interactions (Collmer and Keen, 1986;Johnston and Williamson, 1992;Leone, 1992); cellulase and a trans-eliminase (Verhoeff and Warren, 1972); xylanases, arabinase, /3-glucosidase, /3-galactosidase,/3-mannosidase, and a-galactosidase (Urbanek and Zalewska-Sobczak, 1984).…”

Section: The Pathogen and Diseases Caused By Itmentioning

confidence: 99%

Mechanisms involved in the biological control ofBotrytis cinerea incited diseases

Elad

1996

Eur J Plant Pathol

139

View full text Add to dashboard Cite

Mechanisms involved in the biological suppression of infection and inoculum potential of Botrytis cinerea are numerous and variable and the involvement of two or more mechanisms has been demonstrated in several systems. Reported combinations include antibiosis with enzyme degradation of B. cinerea cell walls; competition for nutrients followed by interference with pathogenicity enzymes of the pathogen or with induced resistance; and alteration of plant surface wettability combined with antibiosis. Since germinating B. cinerea conidia are dependent on the presence of nutrients, competition for nutrients is regarded as important in systems where biocontrol is involved. Conidial viability and germination capacity are also potentially affected by the presence of antibiotics produced by biocontrol agents and present in the phyllosphere. Slower in action are mechanisms involving induced resistance in the host plant and production of hydrolytic enzymes that degrade B. cinerea cell walls. The latter has been demonstrated much more convincingly in vitro than in the phyllosphere. Biocontrol in established lesions and reduction of sporulation on necrotic plant tissues is a means to minimize the pathogen inoculum.

show abstract

Multiplicity of Cell Wall Degrading Glycosidic Hydrolases Produced by Apple Infecting Botrytis cinerea

Cited by 22 publications

References 19 publications

Impact of Botrytis cinerea Contamination on the Characteristics and Foamability of Yeast Macromolecules Released during the Alcoholic Fermentation of a Model Grape Juice

Impact of Botrytis cinerea Contamination on the Characteristics and Foamability of Yeast Macromolecules Released during the Alcoholic Fermentation of a Model Grape Juice

Identification and characteristics of Stagonosporopsis cucurbitacearum pathogenic factors influencing pumpkin seeding survival in north‐east China

Mechanisms involved in the biological control ofBotrytis cinerea incited diseases

Contact Info

Product

Resources

About