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It was shown that Botrytis cinerea, an isolate infecting apples, secreted in vivo and in culture a variety of glycosidic hydrolases with substrate specificity towards the polysaccharides of cell walls. The following enzymes were partially separated by column chromatography on DEAE‐Sepharose CL‐6B: two cellulases, three xylanases, one arabinanase, polygalacturonase, β‐glucosidase, β‐xylosidase, β‐galactosidase, β‐mannosidase and α‐galactosidase. The activity of glycosidic hydrolases tested was strongly inactivated by NBS and weaker by PCMB, tetranitromethan, dibromoacetophenon and Fe3+, The results indicate synergistic action of the obtained cellulase, xylanase, polygalacturonase and arabinanase in apple cell wall degradation.