Comparison of Pectinase, Xylanase and Acid Protease Activities of Virulent ad Less Virulent Isolates of <i>Botrytis cinerea</i>

Zalewska-Sobczak, Jadwiga; Borecka, H.; Urbanek, H.

doi:10.1111/j.1439-0434.1981.tb03343.x

Cited by 17 publications

(7 citation statements)

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“…Support for this hypothesis has also been communicated by others (Legendre 2000, Manteau et al 2000. Secretion of proteases by B. cinerea has been observed in culture media and on fruits such as apple (Zalewska-Sobczak et al 1981) and tomato (Brown and Adikaram 1983). In glasshouse experiments, Monteiro et al (2003b) observed increased levels of thaumatin-like proteins in berries infected with U. necator compared to uninfected berries.…”

Section: Introductionmentioning

confidence: 99%

The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine

Girbau

Stummer

Pocock

et al. 2004

Aust J Grape Wine Res

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Powdery mildew on Chardonnay grapes resulted in increased levels of a grape thaumatin‐like protein, VvTL2, in the free run juice compared to that from uninfected grapes. These increased levels persisted through winemaking and at the highest level of infection (> 30% of bunches infected) had a significant impact on the haziness in the wine following a heat test. Infection of Cabernet Sauvignon grapes (1–20% of bunches infected) did not affect the protein concentration of free run juice, and only traces of pathogenesis‐related (PR) proteins remained detectable in the Cabernet Sauvignon wines from either infected or healthy grapes. In contrast, infection of Chardonnay or Semillon grapes by Botrytis cinerea in the vineyard resulted in decreased levels of all PR proteins in the free run juice and in a total protein extract from infected berries compared to that from uninfected grapes. Similar trends were observed when B. cinerea was grown in the laboratory on surface‐sterilised berries or in filter‐sterilised juice.

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Section: Introductionmentioning

confidence: 99%

The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine

Girbau

Stummer

Pocock

et al. 2004

Aust J Grape Wine Res

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“…On apple homogenate the fungus started to grow and secrete enzymes a few days later than on a medium composed of apple extract supplemented with pectin and peptone which was used in our previous work (ZALEWSKA-SOBCZAK and URBANEK 1981). As most hydrolases under investigations showed the highest activity in day 10 cultures it seemed advisable to use these for purification.…”

Section: Discussionmentioning

confidence: 93%

“…Bancroft. Cultures were made in 300 mi Erlenmayer flasks containing 20 g of apple homogenate, sterilized by autoclaving and then inoculated with 1 mi of spore suspension prepared as described earlier (ZALEWSKA-SOBCZAK et al 1981). Duplicate flasks were removed at two day intervals.…”

Section: Culture Conditionsmentioning

confidence: 99%

“…Bancroft were infected by point inoculation with the fungus harvested from PDA slant cultures and kept at 20 ^C. At two day intervals the diseased tissues were removed and the supernatant from them was obtained as described earlier (ZALEWSKA-SOBCZAK et al 1981). For purification of enzymes the 10 day cultures on apple homogenate were used.…”

Section: Culture Conditionsmentioning

confidence: 99%

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Multiplicity of Cell Wall Degrading Glycosidic Hydrolases Produced by Apple Infecting Botrytis cinerea

Urbanek

Zalewska-Sobczak

1984

Journal of Phytopathology

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It was shown that Botrytis cinerea, an isolate infecting apples, secreted in vivo and in culture a variety of glycosidic hydrolases with substrate specificity towards the polysaccharides of cell walls. The following enzymes were partially separated by column chromatography on DEAE‐Sepharose CL‐6B: two cellulases, three xylanases, one arabinanase, polygalacturonase, β‐glucosidase, β‐xylosidase, β‐galactosidase, β‐mannosidase and α‐galactosidase. The activity of glycosidic hydrolases tested was strongly inactivated by NBS and weaker by PCMB, tetranitromethan, dibromoacetophenon and Fe3+, The results indicate synergistic action of the obtained cellulase, xylanase, polygalacturonase and arabinanase in apple cell wall degradation.

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“…Secretion of proteases by B. cinerea has been observed in culture media and on fruits such as apple (Zalewska-Sobczak et al, 1981) and tomato (Brown and Adikaram, 1983). Girbau et al (2004) also examined the impact of infection of grapes with B. cinerea in the vineyard and showed that infection resulted in marked decreases in the levels of PR proteins in the berries.…”

Section: Effect Of Grape Fungal Infectionmentioning

confidence: 96%

New directions in stabilization, clarification and fining of white wines

Marchal

Waters

2010

Managing Wine Quality

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Comparison of Pectinase, Xylanase and Acid Protease Activities of Virulent ad Less Virulent Isolates of Botrytis cinerea

Cited by 17 publications

References 9 publications

The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine

The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine

Multiplicity of Cell Wall Degrading Glycosidic Hydrolases Produced by Apple Infecting Botrytis cinerea

New directions in stabilization, clarification and fining of white wines

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