Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

Nurkapita, Nurkapita; Linda, Riza; Zakiah, Zulfa

doi:10.35799/jbl.11.2.2021.32674

Cited by 2 publications

(1 citation statement)

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“…ZPT juga dapat diperoleh dari berbagai bahan organik yang tersedia di alam seperti buah-buahan. Menurut Nurkapita et al, (2021) penambahan bahan organik yang memiliki kandungan ZPT dan vitamin dalam media kultur in vitro dapat menstimulasi pertumbuhan tanaman lebih baik. ZPT alami dalam media kultur in vitro misalnya air kelapa yang mampu meningkatkan multiplikasi tanaman secara in vitro yang didukung dari zeatin, sitokinin, auksin, vitamin dan mineral (Pratama and Nilahayati, 2018).…”

Section: Pendahuluanunclassified

Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro

Kusuma

Samsurianto²,

Azizah

et al. 2023

Paspalum

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Anggrek Tebu (Grammatophyllum scriptum) propagation can be improved optimally by adding plant growth regulators such as BAP and NAA through in vitro technique. Murashige and Skoog (MS) medium is an essential medium for plant tissue growth that needs another complex mineral and vitamin to develop the protocorm-like bodies (PBL) of Anggrek Tebu. Hence, this study aimed to gain the appropriate composition of modified MS by adding BAP and various coconut water concentrations and to evaluate their effect on PBL of Anggrek Tebu growth (budding time, number of buds, and leaves). The research was conducted using a completely randomized design with two factorials such as modified MS medium (MS (M1), ½ MS (M2), and ¼ MS (M3))and coconut water concentration (0 ml/L (C0), 100 ml/L (C1), 200 ml /L (C2), and 300 ml/L (C3)) with triplication. The result showed that the fastest budding time was remarkable growth from M2C1 offered the bud at three weeks after planting (WAP). In the number of budding, M3C2 showed excellent results and significantly increased the budding growth at 8.33 ± 0.88 in 10 WAP. Meanwhile, the highest leave number was M2C1 at 23.00 ± 3.60, followed by M2C2 and M3C2, respectively, at 20.67 ± 1.45 and 20.67 ± 1.33. This result indicated that modifying MS medium composition by adding the coconut water could assist the PBL of Anggrek Tebu growth in a specific concentration.

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Section: Pendahuluanunclassified

Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro

Kusuma

Samsurianto²,

Azizah

et al. 2023

Paspalum

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The effect of indole-3-acetic-acid and benzyl amino purine concentrations on matoa (Pometia pinnata) growth in vitro

Yuniastuti

Pratami

Sukaya

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Matoa, a Papuan fruit, is known to taste like a cross between longan, rambutan, and durian. Therefore, Matoa has seed storage and plant propagation restrictions. The tissue culture method of matoa propagation is used to solve this issue. This research aims to provide vegetative planting material that can be propagated in vitro. The study was conducted from June 2021 to January 2022 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Sebelas Maret University, Surakarta, using a completely randomized design pattern (CRD) 2x4 treatment factors for growth regulators. There were 16 treatment combinations, which were repeated three times. The first factor is Indole Acetic Acid (IAA) concentration, which has four levels: 0 ppm, 0.5 ppm, 1 ppm, and 1.5 ppm. The second factor is Benzyl Amino Purine (BAP) concentration, which has four levels: 0 ppm, 2 ppm, 4 ppm, and 6 ppm. The results revealed that root emergence time, leaf emergence time, root number, and leaf number all had high growth rates. The number of leaves and stem nodes correlated, indicating that matoa explant growth was linked.

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Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

Cited by 2 publications

References 0 publications

Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro

Respon Pertumbuhan Protocorm Like Bodies (PLB) Anggrek Tebu (Grammatophyllum scriptum Blume) terhadap Modifikasi Media Murashige and Skoog (MS) secara In Vitro

The effect of indole-3-acetic-acid and benzyl amino purine concentrations on matoa (Pometia pinnata) growth in vitro

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