Multipotent mesenchymal stem cells from adult human synovial membrane

Bari, Cosimo De; Dell’Accio, Francesco; Tylzanowski, Przemko; Luyten, Frank P.

doi:10.1002/1529-0131(200108)44:8<1928::aid-art331>3.0.co;2-p

Cited by 1,488 publications

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References 46 publications

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“…There are increasing reports that MSCs can be isolated from various adult mesenchymal tissues such as synovium (5), periosteum (6), skeletal muscle (7), and adipose tissue (8) in addition to bone marrow (9). These MSCs have been assumed to be similar irrespective of their original tissue source since they all have self-renewal and multidifferentiation potential with common surface epitopes (10,11).…”

mentioning

confidence: 99%

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Sakaguchi¹,

Sekiya²,

Yagishita³

et al. 2005

Arthritis & Rheumatism

1,375

1,171

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Objective. To compare the properties of human mesenchymal stem cells (MSCs) isolated from bone marrow, synovium, periosteum, skeletal muscle, and adipose tissue.Methods. Human mesenchymal tissues were obtained from 8 donors during knee surgery for ligament injury. After collagenase digestion or gradient-density separation, nucleated cells were plated at an appropriate density for expansion at the maximum rate without colony-to-colony contact. Yield, expandability, differentiation potential, and epitope profile were compared among MSCs from the 5 different tissue sources.Results. Colony number per 10 3 nucleated cells was lower, and cell number per colony was higher, in bone marrow than in other mesenchymal tissues. When the cells were replated at low density every 14 days, bone marrow-, synovium-, and periosteum-derived cells retained their proliferation ability even at passage 10. In chondrogenesis studies in which the cells were pelleted and cultured in vitro, pellets from bone marrow-, synovium-, and periosteum-derived cells were shown to be larger and stained more extensively for cartilage matrix. Synovium-derived cells, in particular, had the greatest ability for chondrogenesis. In adipogenesis experiments, the frequency of oil red O-positive colonies was highest in synovium-and adipose tissue-derived cells. In studies of osteogenesis, the rate of alizarin red-positive colonies was highest in bone marrow-, synovium-, and periosteum-derived cells. For epitope profiling, 15 surface antigens were measured. Most appeared to have similar epitope profiles irrespective of cell source.Conclusion. Our findings indicate that there are significant differences in MSC properties according to tissue source, beyond donor and experimental variation. Superiority of synovium as a potential source of MSCs for clinical applications was demonstrated.

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mentioning

confidence: 99%

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Sakaguchi¹,

Sekiya²,

Yagishita³

et al. 2005

Arthritis & Rheumatism

1,375

1,171

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“…Indeed, cells with analogous biologic features have been isolated from other tissues, including synovial membrane (26,73), synovial fluid (33), periosteum (74,75), trabecular bone (76), and adipose tissue (77). Regardless of their source, MSCs possess multipotency and appear to have distinctive features in vitro and in vivo.…”

Section: Development Of Novel Therapies Based On Msc Tissue Specificimentioning

confidence: 99%

Lessons from musculoskeletal stem cell research: The key to successful regenerative medicine development

McGonagle

Bari

Arnold

et al. 2007

Arthritis & Rheumatism

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Polystyrene and poly(butyl acrylate) were grafted from silicon wafer surface by reversible addition‐fragmentation chain transfer (RAFT) polymerization. Three RAFT agents were immobilized onto silicon wafer through their leaving/initiating groups (R group). Grafting polymerization of butyl acrylate (BA) and styrene (St) was then carried out from the immobilized RAFT agents. The immobilization of the RAFT agents and the subsequent grafting polymerization of St and BA were evaluated by ellipsometry and X‐ray photoelectron spectroscopy. It was found that type of monomer, structure of RAFT agent, and local RAFT concentration on the surface have dramatic influences on the thickness of grafted polymer layer. The grafting polymerization with more severe rate retardation effect yielded thinner polymer films on the silicon wafer. Selection of a RAFT agent with little rate retardation was critical in the grafting polymerization to achieve thick films. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 970–978, 2008

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“…Subsequent studies showed that although MSCs reside mainly in bone marrow, they can be isolated also from other adult and fetal tissues including adipose tissue, umbilical cord, fetal lung, amniotic fluid, placenta, synovial membranes, peripheral blood, and dental pulp (Erices et al 2002;Campagnoli et al 2001;De Bari et al 2001;Int Anker et al 2004;Wagner et al 2005;Huang et al 2009;Ab Kadir et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

Giacoppo

Bramantı

Mazzon

2017

Arch. Immunol. Ther. Exp.

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Multipotent mesenchymal stem cells from adult human synovial membrane

Cited by 1,488 publications

References 46 publications

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Comparison of human stem cells derived from various mesenchymal tissues: Superiority of synovium as a cell source

Lessons from musculoskeletal stem cell research: The key to successful regenerative medicine development

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

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