Multipotent progenitor cells can be isolated from postnatal murine bone marrow, muscle, and brain

Jiang, Yuehua; Vaessen, Ben; Lenvik, Todd; Blackstad, Mark; Reyes, Morayma; Verfaillie, Catherine M.

doi:10.1016/s0301-472x(02)00869-x

Cited by 784 publications

(588 citation statements)

References 48 publications

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“…In addition to satellite cells that were previously thought to be the only cell source for muscle regeneration, other cell types, such as SP cells and MAPCs in various tissues, possibly originating from the vascular endothelium, may also contribute to muscle repair. 17,19,35,36 We have previously shown that lentiviral vectors mediate microdystrophin gene transfer to mdx muscle SP cells, and that transduced SP cells can deliver dystrophin genes to regenerating mdx muscle fibers following tail vein transplantation. 36 Kobinger et al 15 showed that lentiviral vectors can transduce satellite cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…Mouse MAPCs from bone marrow and skeletal muscle possess myogenic potential. 19 We examined whether lentiviral vectors could effectively transduce mouse MAPCs. MAPCs isolated from wild-type mouse skeletal muscle were transduced with L-MSCV-eGFP vectors at an MOI of 10.…”

Section: Genetically Modified MDX Myoblasts Retain Myogenic Potentialmentioning

confidence: 99%

“…19 The multipotent differentiation capacity of isolated MAPCs was examined by in vitro differentiation assay 19 before and after viral vector transduction.…”

Section: Isolation Of Muscle Mapcsmentioning

confidence: 99%

“…16 Recently, new populations of stem cells with myogenic potential have been identified. [17][18][19] Vectors with an ability to target these myogenic progenitors could be useful for ex vivo gene therapy of muscle diseases by enabling the generation of genetically modified stem cells for muscle regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Stable transduction of myogenic cells with lentiviral vectors expressing a minidystrophin

Kimura

Fall

et al. 2005

Gene Ther

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Gene therapy for Duchenne muscular dystrophy (DMD) will require sustained expression of therapeutic dystrophins in striated muscles. Lentiviral vectors have a relatively large transgene carrying capacity and can integrate into nondividing cells. We therefore explored the use of lentiviral vectors for transferring genes into mouse skeletal muscle cells. These vectors successfully transferred a minidystrophin expression cassette into mdx muscles, and minidystrophin expression persisted and prevented subsequent muscle fiber degeneration for at least 6 months. However, only low to moderate levels of skeletal muscle transduction could be obtained by intramuscular injection of the highest currently available lentiviral doses. Using cultured cells, the lentiviral vectors effectively transduced proliferating and terminally differentiated muscle cells, indicating that cell cycling is not essential for transduction of myogenic cells. We further showed that lentiviral vectors efficiently transduced both primary myoblasts and multipotent adult progenitor cells (MAPCs) in vitro, and the cells persistently expressed transgenes without any obvious toxicity. When mdx primary myoblasts were genetically modified with minidystrophin vectors and transplanted into mdx skeletal muscles, significant numbers of dystrophin-expressing myofibers formed. Finally, we showed that a short, highly active CK6 regulatory cassette directed muscle-specific activity in the context of the lentiviral vectors. The ability of lentiviral vectors to transduce myogenic progenitors using a minidystrophin cassette regulated by a muscle-specific promoter suggests that this system could be useful for ex vivo gene therapy of muscular dystrophy.

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Section: Discussionmentioning

confidence: 99%

Section: Genetically Modified MDX Myoblasts Retain Myogenic Potentialmentioning

confidence: 99%

“…19 The multipotent differentiation capacity of isolated MAPCs was examined by in vitro differentiation assay 19 before and after viral vector transduction.…”

Section: Isolation Of Muscle Mapcsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stable transduction of myogenic cells with lentiviral vectors expressing a minidystrophin

Kimura

Fall

et al. 2005

Gene Ther

View full text Add to dashboard Cite

show abstract

“…A population of highly plastic, adult derived bone marrow cells, referred to as mulitpotent adult progenitor cells (MAPC) can be grown in vitro from the post-natal marrow of mice, rats and humans [14]. Unlike MSC, MAPC can be cultured indefinitely in a relatively nutrient-poor medium.…”

Section: Multipotent Adult Progenitor Cellsmentioning

confidence: 99%