Multipotent stem and progenitor cells of the olfactory epithelium

Viktorov, I. V.; Savchenko, Ekaterina; Ukhova, O V; Alekseyeva, N Yu; Chekhonin, V. P.

doi:10.1007/s10517-006-0402-y

Cited by 9 publications

(9 citation statements)

References 123 publications

(175 reference statements)

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“…These results are in agreement with previous publications indicating that NOM tissue is a heterogeneous complex. 35 Moreover, recent studies indicate that the transplantation of the "pure" OEC population is insufficient for attaining complete recovery of spinal cord injuries. A better recovery of the structure, and sensory and motor functions of an injured spinal cord can be attained using a complex of NOM cells, including, apart from the OECs, multipotent stem and progenitor cells.…”

Section: Resultsmentioning

confidence: 99%

“…A better recovery of the structure, and sensory and motor functions of an injured spinal cord can be attained using a complex of NOM cells, including, apart from the OECs, multipotent stem and progenitor cells. 35 The clotting time of the fibrin hydrogel depends mainly on the concentration of the fibrinogen, thrombin and CaCl 2 . 5,6,47 In our developed formulation, the clotting time is about 2 minutes.…”

Section: Resultsmentioning

confidence: 99%

“…For example, adult nasal olfactory mucosa (NOM) cells are primary cells of interest for implantation into spinal cord injuries. 3,[34][35][36][37][38][39] NOM cells are often derived through a simple nasal biopsy from the patient prior to implantation in his or her own body, which eliminates rejection. The NOM is a heterogeneous complex of cells which includes, among others, neuronal progenitor cells (adult stem cells), and glial olfactory ensheathing cells (OECs).…”

Section: Introductionmentioning

confidence: 99%

“…The NOM is a heterogeneous complex of cells which includes, among others, neuronal progenitor cells (adult stem cells), and glial olfactory ensheathing cells (OECs). 35 NOM cells have been investigated extensively to see if they have the same capacity to promote neuronal regeneration following spinal cord injuries. 34 Some of these studies showed that the transplantation of OECs could promote axonal regeneration and functional recovery through their specific glia properties, namely, by secretion of neurotrophic factors (such as nerve growth factor [NGF], brain-derived neurotrophic factors [BDNF] and glial cell-derived neurotrophic factor [GDNF]) and extracellular matrix molecules (such as N-CAM and laminin).…”

Section: Introductionmentioning

confidence: 99%

“…A better recovery of these injuries can be attained using a complex of NOM cells, including, apart from the OECs, neuronal progenitor cells from the neuroepithelium. 35,37 We describe herein the development of new magnetic fibrin hydrogel scaffolds intended for the cultivation and implantation of NOM cells into spinal cord injuries. The scaffolds are obtained by the interaction of thrombin-conjugated γ-Fe 2 O 3 magnetic nanoparticles and fibrinogen.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

Ziv-Polat¹,

Skaat²,

Shahar³

et al. 2012

IJN

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Novel tissue-engineered magnetic fibrin hydrogel scaffolds were prepared by the interaction of thrombin-conjugated iron oxide magnetic nanoparticles with fibrinogen. In addition, stabilization of basal fibroblast growth factor (bFGF) was achieved by the covalent and physical conjugation of the growth factor to the magnetic nanoparticles. Adult nasal olfactory mucosa (NOM) cells were seeded in the transparent fibrin scaffolds in the absence or presence of the free or conjugated bFGF-iron oxide nanoparticles. The conjugated bFGF enhanced significantly the growth and differentiation of the NOM cells in the fibrin scaffolds, compared to the same or even five times higher concentration of the free bFGF. In the presence of the bFGFconjugated magnetic nanoparticles, the cultured NOM cells proliferated and formed a threedimensional interconnected network composed mainly of tapered bipolar cells. The magnetic properties of these matrices are due to the integration of the thrombin-and bFGF-conjugated magnetic nanoparticles within the scaffolds. The magnetic properties of these scaffolds may be used in future work for various applications, such as magnetic resonance visualization of the scaffolds after implantation and reloading the scaffolds via magnetic forces with bioactive agents, eg, growth factors bound to the iron oxide magnetic nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

Ziv-Polat¹,

Skaat²,

Shahar³

et al. 2012

IJN

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Neural Progenitor and Hemopoietic Stem Cells Inhibit the Growth of Low-Differentiated Glioma

Baklaushev

Grinenko²,

Savchenko³

et al. 2012

Bull Exp Biol Med

Self Cite

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The effects of neural progenitor and hemopoietic stem cells on C6 glioma cells were studied in in vivo and in vitro experiments. Considerable inhibition of proliferation during co-culturing of glioma cells with neural progenitor cells was revealed by quantitative MTT test and bromodeoxyuridine incorporation test. Labeled neural progenitor and hemopoietic stem cells implanted into the focus of experimental cerebral glioma C6 survive in the brain of experimental animals for at least 7 days, migrate with glioma cells, and accumulate in the peritumoral space. Under these conditions, neural progenitor cells differentiate with the formation of long processes. Morphometric analysis of glioma cells showed that implantation of neural progenitor and hemopoietic stem cells is accompanied by considerable inhibition of the growth of experimental glioma C6 in comparison with the control. The mechanisms of tumor-suppressive effects of neural and hemopoietic stem cells require further investigation.

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Gliotoxicity of the cyanotoxin, β-methyl-amino-L-alanine (BMAA)

Chiu

Gehringer

Braidy

et al. 2013

Sci Rep

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The amino acid variant β-methyl-amino-L-alanine (BMAA) has long been associated with the increased incidence and progression of the amyotrophic lateral sclerosis/Parkinsonism dementia complex (ALS/PDC). Previous studies have indicated that BMAA damages neurons via excitotoxic mechanisms. We have challenged rat olfactory ensheathing cells (OECs) with exogenous BMAA and found it to be cytotoxic. BMAA also induces a significant increase in Ca2+ influx, enhanced production of reactive oxygen species (ROS), and disrupts mitochondrial activity in OECs. This is the first study investigating BMAA toxicity using pure glial cells. These findings align BMAA with the three proposed mechanisms of degeneration in ALS, those being non-cell autonomous death, excitotoxicity and mitochondrial dysfunction.

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Multipotent stem and progenitor cells of the olfactory epithelium

Cited by 9 publications

References 123 publications

Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

Novel magnetic fibrin hydrogel scaffolds containing thrombin and growth factors conjugated iron oxide nanoparticles for tissue engineering

Neural Progenitor and Hemopoietic Stem Cells Inhibit the Growth of Low-Differentiated Glioma

Gliotoxicity of the cyanotoxin, β-methyl-amino-L-alanine (BMAA)

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