Multipotent stem cell factor UGS148 is a marker for tanycytes in the adult hypothalamus

San, Ming; Brouwer, Nieske; Wesseling, Evelyn; Raj, Divya; Want, J.J.L. van der; Boddeke, Erik; Balasubramaniyan, Veerakumar; Copray, Sjef

doi:10.1016/j.mcn.2015.02.002

Cited by 13 publications

(21 citation statements)

References 34 publications

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“…To further characterize the putative neurogenic potential of the hypothalamic NSCP we continued analyzing the proliferative state of hypothalamic tanycytes [23, 24]. We found proliferative cells in hypothalamic areas surrounding the 3 rd ventricle.…”

Section: Resultsmentioning

confidence: 99%

Early life stress decreases the proliferation and numbers of adult hypothalamic neural stem cells

Bielefeld

Abbink

Davidson

et al. 2019

Preprint

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Early life stress (ELS) is a potent environmental factor that can confer enduring effects on brain structure and function. Exposure to stress during early life has been linked to a wide range of physiopathological consequences later in life. In particular, ELS has been shown to have lasting effects on neurogenesis in the adult brain, suggesting that ELS is a significant regulator of adult neural stem cell function. Here, we investigated the effect of ELS on the numbers and proliferation of neural stem cells in the hypothalamus of adult mice.We show that ELS has long term negative effects on hypothalamic neural stem cell numbers and on their proliferation. Specifically, ELS reduced the total numbers of PCNA+ cells present in hypothalamic areas surrounding the 3 rd ventricle; the numbers of PCNA+/Sox2+/Nestin-GFP+ cells present in the medial eminence at the base of the 3 rd ventricle; and the number of β-tanycytes around the ventral 3rd ventricle, without affecting the numbers of α-tanycytes in more dorsal areas. These results suggest that a reduction of proliferation and tanycyte numbers contributes to the effects of ELS on the hypothalamus and its consequent physiological alterations. 7

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Section: Resultsmentioning

confidence: 99%

Early life stress decreases the proliferation and numbers of adult hypothalamic neural stem cells

Bielefeld

Abbink

Davidson

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…In rats Rattus norvegicus , DARPP‐32 is observed in GABAergic neurons, Purkinje neurons, astrocytes and ependymal cells in the medial habenula, tanycytes and spinal cord RGCs, which have the dopamine D 1 receptor (Ouimet et al , ; Schalling et al , ). DARPP‐32 is highly enriched in mammalian GFAP + and vimentin + tanycytes (Hökfelt et al , ; Fekete et al , ) and subtypes of the tanycytes are multipotent NSPCs (Haan et al , ; Robins et al , ; Ma et al , ). DARPP‐32, however, also appears in the oligodendrocytes of brains with multiple system atrophy (Honjo et al , ).…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical characterisation of neural stem‐progenitor cells from green terror cichlid Aequidens rivulatus

Wen

Chen

Nan

et al. 2016

Journal of Fish Biology

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In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein 32 (DARPP-32), oligodendrocyte transcription factor 2, paired box 6 and sex determining region Y-box 2. The intensity and localisation of these proteins, however, varied among the different NSPCs. Despite being intermediate cells, NSPCs can be divided into radial glial cells, oligodendrocyte progenitor cells (OPC) and neuroblasts by expressing the astrocyte marker glial fibrillary acidic protein (GFAP), OPC marker A2B5 and neuronal markers, including acetyl-tubulin, βIII-tubulin, microtubule-associated protein 2 and neurofilament protein. Nevertheless, astrocytes were polymorphic and were the most dominant cells in the NSPC cultures. By using Matrigel, radial glia exhibiting a long GFAP or DARPP-32 fibre and neurons exhibiting a significant acetyl-tubulin process were obtained. The results confirmed that NSPCs obtained from A. rivulatus brains can proliferate and differentiate into neurons in vitro. Clonal culture can be useful for further studying the distinct NSPCs.

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“…Notably, although specific marker genes (or combinations of marker genes) are used to roughly separate tanycyte subtypes, many genes exhibit a gradient, rather than a clear-cut distribution across tanycyte subpopulations which are consistent with the notion that tanycytes may be composed of continuous cell trajectory with transition zones between different subtypes. Recently two new markers UGS148 and Prss56 have been identified and found to be enriched in tanycytes [18,19]. …”

Section: Tanycyte Genetic Markersmentioning

confidence: 99%

Are Tanycytes the Missing Link Between Type 2 Diabetes and Alzheimer’s Disease?

et al. 2018

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Tanycytes are highly specialized bipolar ependymal cells that line the ventrolateral wall and the floor of the third ventricle in the brain and form a blood-cerebrospinal fluid barrier at the level of the median eminence. They play a pivotal role in regulating metabolic networks that control body weight and energy homeostasis. Due to the glucosensing function of tanycytes, they could be considered as a critical player in the pathogenesis of type 2 diabetes. Genetic fate mapping studies have established the role of tanycytes for the newly detected adult hypothalamic neurogenesis with important implications for metabolism as well as pathophysiology of various neurodegenerative diseases. We believe that a comprehensive understanding of the physiological mechanisms underlying their neuroplasticity, glucosensing, and cross talk with endothelial cells will enable us to achieve metabolic homeostasis in type 2 diabetes patients and possibly delay the progression of Alzheimer's disease and hopefully improve cognitive function.

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Multipotent stem cell factor UGS148 is a marker for tanycytes in the adult hypothalamus

Cited by 13 publications

References 34 publications

Early life stress decreases the proliferation and numbers of adult hypothalamic neural stem cells

Early life stress decreases the proliferation and numbers of adult hypothalamic neural stem cells

Immunocytochemical characterisation of neural stem‐progenitor cells from green terror cichlid Aequidens rivulatus

Are Tanycytes the Missing Link Between Type 2 Diabetes and Alzheimer’s Disease?

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