Multiprotein bridging factor 2 regulates the expression of the <i>fibroin heavy chain</i> gene by interacting with Bmdimmed in the silkworm <i>Bombyx mori</i>

Zhou, Chunyan; Zha, Xingfu; Shi, Pengju; Wei, Shuguang; Wang, Hancheng; Zheng, Renwen; Xia, Qingyou

doi:10.1111/imb.12239

Cited by 16 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, their expression was low during the IV‐M stage. Thus, the periodic synthesis of silk proteins is regulated by some relevant genes, ensuring the normal function of silk gland cells and enhancing protein synthesis during the feeding stages . The ribosomal proteins that were downregulated at the IV‐M stage might contribute to decreased protein synthesis at the translation level, which has been confirmed in a previous study .…”

Section: Discussionsupporting

confidence: 74%

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

et al. 2018

View full text Add to dashboard Cite

The silk gland of silkworm produces silk proteins during larval development. Many studies have long focused on the silk gland of the fifth instar larvae, but few have investigated this gland at other larval stages. In the present study, the silk gland proteomes of the fourth instar and fourth molt are analyzed using liquid chromatography-tandem mass spectrometry. In total, 2654 proteins are identified from the silk gland. A high abundance of ribosomal proteins and RR-motif chitin-binding proteins is identified during day 2 of the fourth instar (IV-2) larval developmental stage, and the expression of cuticular proteins analogous to peritrophin (CPAP)-motif chitin-binding proteins is higher during the fourth molt (IV-M). In all, nine enzymes are found to be involved in the chitin regeneration pathway in the silk gland. Among them, two chitinase and two chitin deacetylases are identified as CPAP-motif proteins. Furthermore, the expression of CPAP3-G, the most abundant CPAP-motif cuticular protein in the silk gland during the IV-M stage, is investigated using western blot and immunofluorescence analyses; CPAP3-G shows a reverse changing trend with chitin in the silk gland. The findings of this study suggest that CPAP-motif chitin-binding proteins are involved in the degradation of the chitin layer in the silk gland. The data have been deposited to the ProteomeXchange with identifier PXD008677.

show abstract

Section: Discussionsupporting

confidence: 74%

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The fibroin s modules contained MSTRG.11402.4 , MSTRG.9312.1 , and MSTRG.1346.1 , which showed high expression levels during the earlier period, in contrast to the fibroin s expression pattern. MSTRG.11402.4 , MBF2 partial transcript, is reportedly involved in fibroin-H expression regulation [ 38 ] and is also involved in nuclear transport in the SG along with FTZ-F1 [ 39 ]. Although Awh isoform PA ( KWMTBOMO00651 ) and Awh isoform PB ( MSTRG.1346.1 ) belonged to the fibroin s modules, their expression patterns were different during the last instar period ( Figure 2 D and Figure 3 D).…”

Section: Discussionmentioning

confidence: 99%

Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors in Bombyx mori

Masuoka

Cao

Jouraku

et al. 2022

Insects

View full text Add to dashboard Cite

Bombyx mori is an important economic insect and an animal model in pharmacomedical research. Although its physiology has been studied for many years, the mechanism via which silk protein genes are regulated remains unclear. In this study, we performed two-step expression screening, namely co-expression network and time-course expression analyses to screen silk protein regulation factors. A co-expression network analysis using RNA-seq data that were obtained from various tissues, including the silk glands of B. mori, was performed to identify novel silk protein regulatory factors. Overall, 91 transcription factors, including some known ones, were found to be co-expressed with silk protein genes. Furthermore, time-course expression analysis during the fifth instar larvae stage revealed that the expression pattern of 13 novel transcription factors was highly relevant to that of silk protein genes and their known regulatory factor genes. In particular, the expression peak of several transcription factors (TFs) was detected before the expression of silk protein genes peak. These results indicated that a larger number of genes than expected may be involved in silk protein regulation in B. mori. Functional analyses of function-unknown transcription factors should enhance our understanding of this system.

show abstract

“…For instance, Dimm directly activates fibH expression by interacting with Sage [ 119 ]. Dimm can also act as a repressor of fibH by interacting with repressor MBF2 [ 120 ]. Sage forms a complex with Fkh to enhance fibH expression [ 121 ].…”

Section: Biological Functionsmentioning

confidence: 99%

Insect Transcription Factors: A Landscape of Their Structures and Biological Functions in Drosophila and beyond

Guo

Qin

Zhou

et al. 2018

IJMS

View full text Add to dashboard Cite

Transcription factors (TFs) play essential roles in the transcriptional regulation of functional genes, and are involved in diverse physiological processes in living organisms. The fruit fly Drosophila melanogaster, a simple and easily manipulated organismal model, has been extensively applied to study the biological functions of TFs and their related transcriptional regulation mechanisms. It is noteworthy that with the development of genetic tools such as CRISPR/Cas9 and the next-generation genome sequencing techniques in recent years, identification and dissection the complex genetic regulatory networks of TFs have also made great progress in other insects beyond Drosophila. However, unfortunately, there is no comprehensive review that systematically summarizes the structures and biological functions of TFs in both model and non-model insects. Here, we spend extensive effort in collecting vast related studies, and attempt to provide an impartial overview of the progress of the structure and biological functions of current documented TFs in insects, as well as the classical and emerging research methods for studying their regulatory functions. Consequently, considering the importance of versatile TFs in orchestrating diverse insect physiological processes, this review will assist a growing number of entomologists to interrogate this understudied field, and to propel the progress of their contributions to pest control and even human health.

show abstract

Multiprotein bridging factor 2 regulates the expression of the fibroin heavy chain gene by interacting with Bmdimmed in the silkworm Bombyx mori

Cited by 16 publications

References 32 publications

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors in Bombyx mori

Insect Transcription Factors: A Landscape of Their Structures and Biological Functions in Drosophila and beyond

Contact Info

Product

Resources

About