2010
DOI: 10.1016/j.bios.2010.06.057
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Multipurpose high-throughput filtering microarrays (HiFi) for DNA and protein assays

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Cited by 6 publications
(7 citation statements)
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“…The quantitative target detection was demonstrated (Figure 6b) with a limit of detection of 50 pmol L −1 (calculated as three times standard deviation of the blank). This compares well with the sensitivity previously demonstrated in our group for microarrays spotted on a porous membrane, highly efficient for probe immobilization, using the same probe/target couple 26. Moreover, the specificity of target capture was demonstrated by dispensing a sample containing a single target sequence on a matrix of hydrogel dots containing two different probe sequences (Figure 6c).…”
Section: Resultssupporting
confidence: 88%
“…The quantitative target detection was demonstrated (Figure 6b) with a limit of detection of 50 pmol L −1 (calculated as three times standard deviation of the blank). This compares well with the sensitivity previously demonstrated in our group for microarrays spotted on a porous membrane, highly efficient for probe immobilization, using the same probe/target couple 26. Moreover, the specificity of target capture was demonstrated by dispensing a sample containing a single target sequence on a matrix of hydrogel dots containing two different probe sequences (Figure 6c).…”
Section: Resultssupporting
confidence: 88%
“…Agitation of aqueous mixtures of analyte by shaking or manual aspiration/dispensing only marginally enhanced detection of live bacteria but had no impact on the detection of Stx1 indicating that intact cells, and not fragments or relatively small biomolecules, were solely influenced by the applied centrifugal force. A prospective alternative to centrifugation may be employing multiwell plates that incorporate biorecognition element arrayed filter membranes, a combination recently exhibited by [ 33 ]. Any process that forces analyte and antibody probe and/or subsequent reporter probe into close association will be advantageous to detection as exhibited by the method herein.…”
Section: Discussionmentioning
confidence: 99%
“…Hi-Fi plate spotting and hybridization procedures were described elsewere . Here, a two-step protocol (detailed in Supporting Information) was used for hybridization and labeling.…”
Section: Methodsmentioning
confidence: 99%
“…Our group previously reported a cost-efficient high-throughput analysis platform (HiFi-assay) for multiparametric nucleic acids assays, on the basis of a colorimetric detection of hybridization. , This tool, initially developed for high-throughput blood group genotyping assays, was designed in order to fulfill end-user needs (i.e., blood qualification centers): (i) the device is fully compatible with robotic platforms and thus answers throughput constrains (thousands of samples tested per day), and (ii) it is based on an enzymatic colorimetric detection process using alkaline phosphatase as label which reduces the cost per test. Although we obtained very satisfying results, detection can still be further improved.…”
mentioning
confidence: 99%