Multiresidue Chromatographic Method for the Determination of Macrolide Residues in Muscle by High-Performance Liquid Chromatography with UV Detection

Gaugain, Murielle; Anger, Béatrice; Laurentie, Michel

doi:10.1093/jaoac/82.5.1046

Cited by 39 publications

(14 citation statements)

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“…To test the validity of the proposed method, it was applied to the determination of pure samples of TS and JM over the range of 1.0–200.0 μg mL −1 and 5.0–500.0 μg mL −1 , respectively. The results obtained were in good agreement with those obtained using the comparison HPLC method [ 13 ]. Using Student’s t-test and the variance ratio F-test revealed no significant difference between the performance of the two methods regarding the accuracy and precision, respectively (Table 2 ) [ 33 ].…”

Section: Resultssupporting

confidence: 87%

“…Literature revealed several HPLC methods for the simultaneous determination of TS and JM residues in muscle tissues. TS and JM residues were determined together with other macrolide antibiotics in meat [ 12 ], bovine, porcine and poultry muscles [ 13 ] using UV detection. They were also determined in sheep’s milk using UV-diode array detector [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection: application to baby food and formulae

Nasr

Shalan

Belal

2014

Chemistry Central Journal

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BackgroundTylosin and Josamycin are macrolide antibiotics. They are used in the treatment of pneumonia, arthritis and mastitis in cattle, and mycoplasma infections in poultry. The incorrect use of antibiotics has lead to the presence of antibiotic residues in foods. The residues cause toxic effects on consumers.ResultsA simple and sensitive method was optimized and validated for the analysis of tylosin and josamycin residues in food samples. Analytical separation was performed in less than 10 min using a RP C18 monolithic column with time-programmed UV detection at 287 nm and 232 nm and a micellar solution of 0.17 M sodium dodecyl sulphate, 14% methanol and 0.3% triethylamine in 0.02 M phosphoric acid buffered at pH 4 as the mobile phase. The method was fully validated in accordance with ICH guidelines. The micellar method was successfully applied to quantitatively determine tylosin and josamycin residues in spiked chicken muscles, chicken liver, bovine muscles, liver, milk and eggs. It was also extended to the determination of tylosin and josamycin residues in chicken-based baby food and baby formulae. The compounds were separated by a monolithic column which, on account of its particular structure, could bear higher flow rates than usually found for this kind of analysis. High extraction efficiency for tylosin and josamycin was obtained without matrix interference in the extraction process and in the subsequent chromatographic determination. No organic solvent was used during the pretreatment step. Hence, it is considered an interesting technique for “green” chemistry.ConclusionThe proposed method was validated and successfully applied for the determination of tylosin and josamycin residues in spiked chicken muscles, chicken liver, bovine muscles, liver, milk and eggs. It was also extended to the determination of tylosin and josamycin residues in chicken-based baby food and baby formulae.

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Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection: application to baby food and formulae

Nasr

Shalan

Belal

2014

Chemistry Central Journal

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“…Liquid chromatographic techniques by UV [10][11][12][13][14], DAD [8,15,16], fluorimetric [17] and mass spectrometric [18][19][20][21][22] detection were usually followed to determine spiramycin. Furthermore, analytical methods regarding spiramycin determination based on spectrophotometry [23,24] and capillary electrophoresis coupled with voltammetric detection [25] have already been reported elsewhere.…”

Section: Introductionmentioning

confidence: 99%

Development of a spiramycin sensor based on adsorptive stripping linear sweep voltammetry and its application for the determination of spiramycin in chicken egg samples

Cittan¹

2021

Turk J Chem

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Herein, an adsorptive stripping linear sweep voltammetric technique was described to determine spiramycin, a macrolide antibiotic, using a carboxylic multiwalled carbon nanotubes modified glassy carbon electrode. The main principle of the analytical methodology proposed was based on the pre-concentration of spiramycin by open-circuit accumulation of the macrolide onto the modified electrode surface. As a result of the adsorption affinity of spiramycin to the modified surface, the sensitivity of the glassy carbon electrode was significantly increased for the determination of spiramycin. The electrochemical behavior of spiramycin was evaluated by cyclic voltammetry and the irreversible anodic peak observed was measured as an analytical signal in the methodology. The proposed electrochemical sensing platform was quite linear in the range of 0.100-40.0 µM of spiramycin concentration with a correlation coefficient of 0.9993. The limit of detection and the limit of quantification were 0.028 and 0.094 µM, respectively. The intra and inter-day repeatability of the proposed sensor was within acceptable limits. Finally, the applicability of the electrochemical methodology was examined by determining the drug content of chicken egg samples spiked with spiramycin standard. A rapid and easy extraction technique was performed to extract spiked spiramycin from the egg samples. The extraction technique followed had good recovery values between 85.3 ± 4.0 and 93.4 ± 1.9%.

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“…Similarly, administration of very low doses of some drugs for a prolonged time produce reproductive and teratogenic effects [11]. Not only the macrolides residues in edible products have direct toxic effects but also may lead to allergic reactions and development of resistant bacteria in humans [12].…”

Section: Introductionmentioning

confidence: 99%

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

Said

El‐Nabtity

Selim³

et al. 2016

Zagazig Veterinary Journal

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The aim of the present study was to detect the tilmicosin residues in muscles, kidneys and liver of broiler chickens and to investigate its impact on both cardiac enzymes and some hematological parameters. Seventy broiler chickens were divided into two groups; the first was left as a negative control. The second group was given tilmicosin orally (30 mg/kg BW) once/day for three successive days. Reversed phase-high performance liquid chromatography (RP-HPLC) with UV detector at 287 nm and solid phase extraction were used for detecting drug residues in tissue samples. Results indicated a widespread distribution of tilmicosin in most tested tissues. All tissue samples were considered tilmicosin free at the 9 th day after the last oral dose except liver. Tilmicosin elicited a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume, lymphocytes and eosinophiles. On the other hand, heterophiles count and the mean corpuscular volume were increased significantly. White blood cells, monocytes, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration showed non-significant changes. Levels of all cardiac enzymes (Aspartate aminotransferase, lactate dehydrogenase, creatine kinase-MB and Troponin I) were high.

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Multiresidue Chromatographic Method for the Determination of Macrolide Residues in Muscle by High-Performance Liquid Chromatography with UV Detection

Cited by 39 publications

References 0 publications

Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection: application to baby food and formulae

Simultaneous determination of tylosin and josamycin residues in muscles, liver, eggs and milk by MLC with a monolithic column and time-programmed UV detection: application to baby food and formulae

Development of a spiramycin sensor based on adsorptive stripping linear sweep voltammetry and its application for the determination of spiramycin in chicken egg samples

Detection of Tilmicosin Residues by HPLC and its Effect on Cardiac Enzymes and Hematology in Broiler Chickens

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