Multiresidue Determination of Pesticide Residues in Honey by Modified QuEChERS Method and Gas Chromatography with Electron Capture Detection

Orso, Débora; Martins, Manoel Leonardo; Donato, Filipe F.; Rizzetti, Tiele Medianeira; Kemmerich, Magali; Adaime, Martha B.; Zanella, Renato

doi:10.5935/0103-5053.20140117

Cited by 13 publications

(19 citation statements)

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“…Quintozene 29 and caffeine (both 99%) were employed as surrogate standard (SS) and internal standard (IS), respectively. Standards were prepared in toluene in a concentration of 1000 mg L −1 and stored at −18 °C.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of QuEChERS Sample Preparation and Gas Chromatography Coupled to Mass Spectrometry for the Determination of Pesticide Residues in Grapes

Volpatto

Wastowski

Bernardi

et al. 2016

Journal of the Brazilian Chemical Society

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An effective analytical method for pesticides multiresidue determination in grape samples was developed and validated. A modified quick, easy, cheap, effective, robust and safe (QuEChERS) method was used to extract the target compounds. Several sorbent materials were tested for the clean-up step using dispersive solid phase extraction (d-SPE) and Florisil ® was selected. Samples extracts were evaporated before injection in the gas chromatography with mass spectrometry (GC-MS) system in order to improve detectability. Recoveries from blank samples spiked at 0.04, 0.3 and 1.0 mg kg −1 ranged from 95 to 102% with relative standard deviation (RSD) from 1.3 to 19.7%. Method limits of detection (LODm) and quantification (LOQm) ranged from 0.006 to 0.012 and 0.02 to 0.04 mg kg −1 , respectively. The positive matrix effect caused an increase in the peak areas of all compounds and matrix matched calibration curve linearity (coefficient of determination, r 2 ) was higher than 0.98 for all target analytes. The validated method was successfully applied for the determination of 19 pesticides in grape samples.

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Section: Methodsmentioning

confidence: 99%

Evaluation of QuEChERS Sample Preparation and Gas Chromatography Coupled to Mass Spectrometry for the Determination of Pesticide Residues in Grapes

Volpatto

Wastowski

Bernardi

et al. 2016

Journal of the Brazilian Chemical Society

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“…The monitoring of pesticide residues is essential to evaluate the potential risk to consumer health and also to provide relevant information on the use of chemicals in proximity to beehive cultivation. Analytical methodologies for pesticides identification and quantification are recent, and the majority of the published reports refers to the honey of A. mellifera (Blasco, Font, & Picó, 2007;Orso et al, 2014Orso et al, , 2016Tette et al, 2016). There are various methods to detect pesticides in foods, and the QuEChERS method, whose acronym means Quick, Easy, Cheap, Effective, Rugged, and Safe, is one of the most suitable among them (Anastassiades, Lehotay, Stajnbaher, & Schenck, 2003;Prestes, Friggi, Adaime, & Zanella, 2009).…”

Section: Introductionmentioning

confidence: 99%

Quality of Meliponinae honey: Pesticides residues, pollen identity, and microbiological profiles

Rodrigues

Ferasso

Prestes

et al. 2018

Environmental Quality Mgmt

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This study aims at evaluating the quality of honey of Melipona quadrifasciata quadrifasciata Tetragonisca angustula and Scaptotrigona depilis. Pollen analysis was performed to identify the floral resources used for the production of honey, and 19 pollen types were identified. Microbiological analyses identified the presence of coliforms, molds, and yeasts, but Clostridium botulinum was not found. Using the modified “Quick, Easy, Cheap, Effective, Rugged, and Safe” method followed by ultra‐high performance liquid chromatography‐tandem mass spectrometry determination, eight pesticides were detected in the honey of T. angustula and only two in the honey from S. depilis. The honey of M. q. quadrifasciata did not present pesticide residues. The data pointed out the relevance of good practices to avoid the contamination of honey with sources of microbiological contaminants, which may impair the product's safety for human consumption.

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“…It is considered that there are two forms of contamination of honey: during pollen and nectar collection of bees, indirect contamination and through treatment of hives, in which pesticides can migrate in honey, i.e. direct contamination (Orso et al 2014). The evidence for the presence of pesticide residues in honey (Blasco et al 2003, Orso et al 2014) has impelled the need for setting up monitoring programs for obtaining a proper assessment of human exposure to pesticides through honey consumption (Wallner, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Only few of these methods allow multi-class detection of pesticide residues, as well as their quantification at very low concentration levels. In the recent years MSPD sample preparation has been identified as powerful tool for multi-residual analysis of pesticides in honey samples due to its compatibility both with GC and LC chromatographic systems and mass spectrometric detection systems Orso et al 2014, Rodríguez-López et al 2014Fidente et al 2005;Tanner and Czerwenka, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…direct contamination (Orso et al 2014). The evidence for the presence of pesticide residues in honey (Blasco et al 2003, Orso et al 2014) has impelled the need for setting up monitoring programs for obtaining a proper assessment of human exposure to pesticides through honey consumption (Wallner, 1999). Different national regulations have established maximum permitted concentrations of pesticides (MRLs) in honey, but the lack of harmonized regulative cause problems in international trade (Blasco et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of Multi�class Pesticides in Honey Samples From Macedonia by Ultra High Performance Liquid Chromatography � Tandem Quadrupole Mass Spectrometry

Dimitrieska-Stojković¹,

Angjeleska²,

Goran³

et al. 2016

AgricultForest

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The possible presence of pesticide residues in honey has impelled the need for setting up monitoring programs to determine the proper assessment of human exposure to pesticides. This paper describes an effective multi-class method using a modified QuEChERS sample preparation for detection and quantification of 18 pesticides with Ultra-high-performance liquid chromatography (UHPLC) -tandem quadrupole mass spectrometry. The method was validated according to the requirements laid down in DG SANCO 12571/2013 document. Levels of detection and quantification were lower than the established MRLs, the obtained precision was better than 20 %, and the recovery values were between 74.4 and 104.1 %. Fifty honey samples within the national monitoring program were collected from August to November, 2014 and tested for presence of carbaryl, carbofuran, fenvalerate, cypermethrin, deltamethrin, permethrin, bifenthrin, amitraz, coumaphos, bromopropylate, dichlorvos, diazinone, malathion, parathion, dimethoate, omethoate, methomyl and thiametoxam. Trace levels of methomyl, diazinone, bromopropylate and fenvalerate were detected somewhat above the reporting level for these pesticides (10 µg/kg). This contamination indicates on existing of possible moderate cross-contamination during pollen and nectar collection by bees, and residues of substances used in beekeeping. Although traces of pesticides were found in 16 of the samples tested (32 %), the levels found did not pose increased toxicological risk for the population.

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Multiresidue Determination of Pesticide Residues in Honey by Modified QuEChERS Method and Gas Chromatography with Electron Capture Detection

Cited by 13 publications

References 0 publications

Evaluation of QuEChERS Sample Preparation and Gas Chromatography Coupled to Mass Spectrometry for the Determination of Pesticide Residues in Grapes

Evaluation of QuEChERS Sample Preparation and Gas Chromatography Coupled to Mass Spectrometry for the Determination of Pesticide Residues in Grapes

Quality of Meliponinae honey: Pesticides residues, pollen identity, and microbiological profiles

Monitoring of Multi�class Pesticides in Honey Samples From Macedonia by Ultra High Performance Liquid Chromatography � Tandem Quadrupole Mass Spectrometry

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