Multiscale immunomagnetic enrichment of circulating tumor cells: from tubes to microchips

Chen, Peng; Yu, Haiyang; Hoshino, Kazunori; Zhang, Xiaojing

doi:10.1039/c3lc51107c

Cited by 84 publications

(62 citation statements)

References 92 publications

(127 reference statements)

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“…Integration of an immunomagnetic assay with a microchip is a widely used cell isolation method whereby magnetic force is applied to separate cancer cells (e.g., CTCs), labeled with magnetic particles, from unlabeled red and white blood cells for further molecular analysis. In this approach, magnetic particles/beads coated with antibodies specific for antigens expressed on cancer cells are used to tag target cells for magnetic capture (Figure 4) [61–66]. Dendrimers, polymer nanofibers, graphene, and other nanomaterials have also been introduced in microfluidic chips to increase capture efficiency [67–70].…”

Section: Cell-based Molecular Analysesmentioning

confidence: 99%

Point-of-care technologies for molecular diagnostics using a drop of blood

Song

Liu

et al. 2014

Trends in Biotechnology

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Molecular diagnostics is critical for prevention, identification, and treatment of disease. Traditional technologies for molecular diagnostics using blood are limited to laboratory use because they rely on sample purification and sophisticated instruments, are labor- and time-intensive and expensive, and require highly trained operators. This review discusses the frontiers of point-of-care diagnostic technologies using a drop of blood obtained from a finger-prick. These technologies, including emerging biotechnologies, nanotechnologies, and microfluidics, hold the potential for rapid, accurate, and inexpensive disease diagnostics.

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Section: Cell-based Molecular Analysesmentioning

confidence: 99%

Point-of-care technologies for molecular diagnostics using a drop of blood

Song

Liu

et al. 2014

Trends in Biotechnology

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200

145

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“…The aggregation may impact the structural integrity and fluorescent signals of the CTC, and interfere with cell imaging and identifying. To advance the immunomagnetic assay, especially for rare cell detection applications, integration of microfluidic chip and microscale magnetic flux source has been pursued [5]. …”

Section: Introductionmentioning

confidence: 99%

Inkjet-Print Micromagnet Array on Glass Slides for Immunomagnetic Enrichment of Circulating Tumor Cells

Chen

Bhave

et al. 2015

Ann Biomed Eng

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We report an inkjet-printed microscale magnetic structure that can be integrated on regular glass slides for the immunomagnetic screening of rare Circulating Tumor Cells (CTCs). CTCs detach from the primary tumor site, circulate with the bloodstream, and initiate the cancer metastasis process. Therefore, a liquid biopsy in the form of capturing and analyzing CTCs may provide key information for cancer prognosis and diagnosis. Inkjet printing technology provides a non-contact, layer-by-layer and mask-less approach to deposit defined magnetic patterns on an arbitrary substrate. Such thin film patterns, when placed in an external magnetic field, significantly enhance the attractive force in the near-field close to the CTCs to facilitate the separation. We demonstrated the efficacy of the inkjet-print micromagnet array integrated immunomagnetic assay in separating COLO205 (human colorectal cancer cell line) from whole blood samples. The micromagnets increased the capture efficiency by 26% compared with using plain glass slide as the substrate.

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“…Immunomagnetic assay has shown clinical significance in cancer diagnosis and prognosis. [104][105][106][107][108][109] The generation and fine-tuning of the magnetic field play essential roles in such assay toward effective singlecell-based analyses.…”

Section: Immunomagneticsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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Multiscale immunomagnetic enrichment of circulating tumor cells: from tubes to microchips

Cited by 84 publications

References 92 publications

Point-of-care technologies for molecular diagnostics using a drop of blood

Point-of-care technologies for molecular diagnostics using a drop of blood

Inkjet-Print Micromagnet Array on Glass Slides for Immunomagnetic Enrichment of Circulating Tumor Cells

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

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