Multispectral Mueller matrix imaging dark-field microscope for biological sample observation

Saito, Naooki; Satô, Kenji; Fujii, Tôru; Durko, Heather L.; Goldstein, Goldie; Phillips, Alton H.; Dominguez-Cooks, Joceline; Hutchens, Gabrielle V.; Thurgood, Harrison T.; Rice, Photini F. S.; Barton, Jennifer K.

doi:10.1117/12.2508109

Cited by 8 publications

(15 citation statements)

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“…The polarization microscope is a custom instrument developed by Nikon (Saito et al, 2019), which consists of an episodic illumination system capable of dark-field illumination in five wavelengths and a polarimetric imaging system for measuring the state of polarization of scattered light from the tissue sample positioned at the microscope's specimen stage (Figures 1B,C). A Nikon bright-field objective lens with minimal polarization distortion (Nikon XFI TU Plan Fluor EPI) was used to operate within the epi-illumination and dark-field imaging constraints of this polarimeter.…”

Section: Polarization Imagingmentioning

confidence: 99%

“…5× magnification was used to capture all images due to its larger field of view and depth of field. The polarimeter uses a pair of Savart plates to measure the four Stokes parameters simultaneously in encoded interference fringes (Saito et al, 2019;Oka and Saito, 2006). In concurrent software, also developed for the system by Nikon, a data reduction algorithm is applied which constructs a measurement matrix based on the illumination and detection polarization states and multiplies the pseudoinverse of that with the measured data to find the least-squares optimized Mueller Matrix of the sample (Chipman, 1994), and a series of calibrations are completed to remove artifacts from the illumination and imaging optics (Saito et al, 2019).…”

Section: Polarization Imagingmentioning

confidence: 99%

“…Polarized light imaging (PLI) is a popular technique used for probing physiological structures in the brain with high spatial resolution, which has shown great promise for brain imaging and dMRI validation (Mehta et al, 2013). PLI is generally capable of higher resolution imaging than MRI, for example the system used in this study has a spatial resolution on the order of 10μm, which while still a bit larger than most axons with a scale of 1-3μm is much closer than that of dMRI which is on the order of hundreds of microns (Hutchinson et al, 2017;Saito et al, 2019;Grisot et al, 2021). In particular, the myelin sheath surrounding neurons is strongly birefringent (Morgan et al, 2021), which will cause a phase delay in polarized light along the axis as the neuronal fibers.…”

Section: Introductionmentioning

confidence: 99%

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Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

et al. 2022

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Understanding microscale physiology and microstructural cellular features of the brain is key to understanding mechanisms of neurodegenerative diseases and injury, as well as prominent changes undergone in development and aging. Non-invasive imaging modalities sensitive to the microscale, especially diffusion magnetic resonance imaging (dMRI), are promising for mapping of cellular microstructure of brain tissues; however, there is a need for robust validation techniques to verify and improve the biological accuracy of information derived. Recent advances in dMRI have moved toward probing of the more complex grey matter architecture, challenging current validation techniques, which are largely based on ex vivo staining and microscopy focusing on white matter. Polarized light imaging (PLI) has been shown to be successful for high resolution, direct, microstructural imaging and has been applied to dMRI validation with clear advantages over staining and microscopy techniques. Conventionally, PLI is applied to thin, sectioned samples in transmission mode, but PLI has also been extended to operate in reflectance mode to bridge the gap toward in vivo measurements of the brain. In this report we investigate the use of backscattering Mueller Matrix polarimetry to characterize the microstructural content of intact ferret brain specimens. The results show that backscattering polarimetry can probe white matter fiber coherence and fiber orientation, and show promise for probing grey matter microstructure. Ultimately, this motivates further study to fully understand how best to implement backscattering polarimetry for in vivo microstructural imaging of the brain.

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Section: Polarization Imagingmentioning

confidence: 99%

Section: Polarization Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

et al. 2022

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“…5X magnification was Frontiers used to capture all images due to its larger field of view and depth of field. The instrument has been previously characterized, validated, and used for human tissue imaging, and produces spatial pixel-wise mappings of the sixteen Mueller Matrix components (Fujii et al (2019); Saito et al (2019)). Exposure times were optimized using the instruments "auto-adjust" setting.…”

Section: Polarization Imagingmentioning

confidence: 99%

Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

Bonaventura

Morara

Carlson

et al. 2022

Preprint

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Understanding microscale physiology and microstructural cellular features of the brain is key to understanding the mechanisms of neurodegenerative diseases and injury, as well as the prominent changes that neurons and glia in the brain undergo in development and aging which reflect functional state. Non-invasive imaging modalities sensitive to the microscale - especially diffusion magnetic resonance imaging (dMRI) - are extremely promising for three-dimensional mapping of cellular microstructure of brain tissues and brain connectivity via tractography; however, there is a need for robust validation techniques to verify and improve the biological accuracy of fiber orientation information derived from these techniques. Recent advances in dMRI acquisition and modeling have moved toward probing of the more complex grey matter architecture, challenging current validation techniques, which are largely based on ex vivo staining and microscopy and focused on white matter. Polarized light imaging (PLI) has been shown to be a successful technique for high resolution, direct, microstructural imaging and has been applied to dMRI validation with clear advantages over conventional staining and microscopy techniques. Conventionally, PLI is applied to thin, sectioned samples in transmission mode, but unlike histologic staining, PLI can be extended to operate with high sensitivity in reflectance mode and even extended to 3D imaging to bridge the gap toward in vivo validation of dMRI measurements of orientation features in both gray and white matter of the brain. In this report we investigate the use of backscattering Mueller Matrix polarimetry to characterize the microstructural content of intact Ferret brain specimens. The experimental results show that backscattering polarimetry can probe white matter fiber coherence and fiber orientation in whole brain specimens, and show promise for probing grey matter microstructure. Ultimately, these preliminary results motivate further study to fully understand how backscattering polarimetry can best be used for validation of in vivo microstructural imaging of the brain.

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“…An imaging polarimeter microscope that operates in a backscattering configuration has been developed for tissue classification and early cancer detection application 12,13 . The difference between the mean measured Mueller matrix values of healthy and cancerous human colon tissue agreed with previously reported results.…”

Section: Introductionmentioning

confidence: 99%

Polarization characteristics of dark-field microscopic polarimetric images of human colon tissue

Fujii

Yamasaki

Saito

et al. 2019

Label-Free Biomedical Imaging and Sensing (LBIS) 2019

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Early detection of cancer through medical imaging has a critical impact on patient survival rates. There are many efforts for detecting early cancer in situ using modalities other than traditional medical optical imaging, which contain additional information over conventional micrographs of surface morphology acquired without staining. We analyzed the Mueller matrix components of human colon tissue obtained with an imaging polarimeter microscope at an illumination wavelength of 442 nm by principal components analysis in order to separate the traditional non-polarized gray image and to investigate the structure of the parameter space of polarization transformation by tissue. We also analyzed Mueller matrix by mapping it to a coherent matrix and performed eigenvalue analysis. The 1 st to 4 th principal components contain 99% of the information present in the images; polarization information contributes less than 10% of the information in the Mueller matrix. In one individual, 80% of the cancer was detected, without the first components which contains traditional non-polarized gray image for traditional diagnosis. Microscopic fine structures were observed, particularly in the 3 rd and 4 th principal components' score images. The entropy image of corrugated cancer tissue was smoother than that of the traditional gray image. There were several abnormal regions identified in adjacent regions of cancer, whose residues exceeded the noise level of the instrument used.

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Multispectral Mueller matrix imaging dark-field microscope for biological sample observation

Cited by 8 publications

References 8 publications

Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

Backscattering Mueller Matrix polarimetry on whole brain specimens shows promise for minimally invasive mapping of microstructural orientation features

Polarization characteristics of dark-field microscopic polarimetric images of human colon tissue

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