Multispectroscopic, electrochemical and molecular docking approaches on binding comparison of camptothecin, 10-hydroxycamptothecin to bovine serum albumin

Yang, Jing; Huang, Su-Dan; Wang, Yi; Ji, Mengyuan; Hu, Yanjun

doi:10.1016/j.molliq.2021.115296

Cited by 28 publications

(4 citation statements)

References 31 publications

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“…There are several modes of interaction between DNA and drugs: non-covalent groove binding, intercalation between the stacked base pairs, and electrostatic interaction with the negatively charged pentose-phosphate structure of DNA . In general, the interactions between drugs and DNA will cause changes in the characteristic absorption of DNA, such as a hyperchromic effect or a hypochromic effect in the ultraviolet spectrum, and a red or a blue shift in the location of absorption peaks, which can be used to preliminarily judge the interaction mechanism . The UV–vis absorption spectra of ctDNA exhibited an absorption band at 256 nm, and upon the addition of both AuNCs, the absorption intensity of ctDNA at 256 nm increased gradually with a slight blue shift (Figure A,B), indicating that the interaction mechanism of two AuNCs and ctDNA might be groove binding .…”

Section: Resultsmentioning

confidence: 99%

Interactions between Two Kinds of Gold Nanoclusters and Calf Thymus Deoxyribonucleic Acid: Directions for Preparations to Applications

Yin

Yang

et al. 2021

Biomacromolecules

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Gold nanoclusters (AuNCs) have shown promising applications in biotherapy owing to their ultrasmall size and unique molecular-like properties. In order to better guide the preparations and applications of AuNCs, dihydrolipoic acid-protected AuNCs (DHLA-AuNCs) and glutathione-protected AuNCs (GSH-AuNCs) were selected as models and the interactions between them and calf thymus DNA (ctDNA) were studied in detail. The results showed that there was a small difference in the binding mechanisms and forces between both AuNCs and ctDNA. The quenching mechanisms of both AuNCs to (ctDNA–HO) were completely different. The binding constants indicated that the binding strength between DHLA-AuNCs and ctDNA was greater than those of GSH-AuNCs. The conformation investigations showed that GSH-AuNCs had a greater impact on the conformation of ctDNA, and both AuNCs were more inclined to interact with the A–T base pairs of ctDNA. These results indicate that the surface ligand had a significant effect on the interactions between AuNCs and DNA and might also further affect the applications of AuNCs, and these results could guide the preparations of AuNCs. For DHLA-AuNCs, their good biocompatibility made them a potential candidate for application in imaging, drug treatment, sensing, and so on. The resulting base accumulation of ctDNA and weak interactions made GSH-AuNCs have great potential for application in gene therapy, which was consistent with the current reports on the applications of these two AuNCs. This work has pointed out the directions for the preparations and applications of AuNCs.

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Section: Resultsmentioning

confidence: 99%

Interactions between Two Kinds of Gold Nanoclusters and Calf Thymus Deoxyribonucleic Acid: Directions for Preparations to Applications

Yin

Yang

et al. 2021

Biomacromolecules

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“…Accordingly, the ratio of lactone form and carboxylate form of the CPT is constant. [51,56,57] The fluorescent spectra of the simulated medium showed that the emission at 432 nm attributed to CPT [53,57,58] gradually enhanced with releasing time, indicating the cumulative concentration of CPT released from ZnAl-Eu-CPT in the simulated medium gradually increased with releasing time (seen in Figure 10).…”

Section: Fluorescent Analysismentioning

confidence: 96%

“…CPT can exist in lactone form (pH < 5.5) or carboxylate form (pH > 9), and the absorption of CPT in the range of 300-400 nm in aqueous solution comes from the two quinoline groups. [51][52][53] Thus, the opening of the lactone ring in carboxylate forms of CPT has little effect on the optical properties of CPT. [8,9] To determine the concentration of CPT released from ZnAl-Eu-CPT in the simulated medium, the linear relationship of the absorbance (λ max = 368 nm) and concentration of the CPT calibrated solution was given in Figure 7.…”

Section: Release Kinetics Of Camptothecinmentioning

confidence: 99%

“…If the ZnAl-Eu-CPT drug delivery system releases of CPT in anionic carboxylate form in acidic gastric fluid, the carboxylate form would be converted into lactone form which has anticancer property because CPT in lactone form exists in acid solution and its carboxylate form exists in basic solution. [51][52][53] As oral drug carrier, it is necessary to evaluate the solubility of the ZnAl-Eu-CPT drug delivery system in simulated medium in consideration of safety. It was found that the amounts of the Zn, Al, and Eu dissolved in the simulated medium are about 3.3 μg/mL, 1.52 μg/mL, and < 1.0 × 10 À 2 μg/mL, respectively, which are far from the level of toxicity.…”

Section: Chemistryselectmentioning

confidence: 99%

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Eu‐doped ZnAl‐LDH as a Fluorescent Labeling Carrier for the Delivery and Controlled Release of Camptothecin

Qiu

et al. 2022

ChemistrySelect

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Europium doped Zinc‐aluminum layered double hydroxide (Eu‐doped ZnAl‐LDH) as a fluorescent labeling carrier for the delivery and controlled release of camptothecin (CPT) has been studied. X‐ray diffraction (XRD) patterns showed that the basal spacing of the Eu‐doped ZnAl‐LDH intercalated with CPT (2.27 nm) was larger than that of the initial Eu‐doped ZnAl‐LDH (0.88 nm), indicating the successful intercalation of CPT into the Eu‐doped ZnAl‐LDH carrier. X‐ray photoelectron spectroscopy (XPS) suggested that some Eu(III) were reduced to Eu(II) after the intercalation of CPT into the Eu‐doped ZnAl‐LDH. The release of the CPT from drug carrier was carried out in a simulated medium. It was found that the cumulative concentration of the released CPT was exponential with releasing time, and well conformed to the exponential equation: C=A‐Bexp(‐kt). It took 720 minutes (min) for the complete release of CPT from drug carrier. Fluorescent analyses revealed that the fluorescence attributed to the drug delivery system changed from red, blue‐green, to blue emission during delivery and release of CPT, which may be a favorable signal for “detecting” the delivery and release of CPT. Thus, the present Eu‐doped ZnAl‐LDH may be a feasible drug carrier with fluorescent labeling function.

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Biopolymer Nanovehicles for Oral Delivery of Natural Anticancer Agents

Falsafi

Wang

Ashaolu

et al. 2022

Adv Funct Materials

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Cancer is the second leading cause of death throughout the world. Nature‐inspired anticancer agents (NAAs) that are a gift of nature to humanity have been extensively utilized in the alleviation/prevention of the disease due to their numerous pharmacological activities. While the oral route is an ideal and common way of drug administration, the application of NAAs through the oral pathway has been extremely limited owing to their inherent features, e.g., poor solubility, gastrointestinal (GI) instability, and low bioavailability. With the development of nano‐driven encapsulation strategies, polymeric vehicles, especially those with natural origins, have demonstrated a potent platform, which can professionally shield versatile NAAs against GI barricades and safely deliver them to the site of action. In this review, the predicament of orally delivering NAAs and the encapsulation strategy solutions based on biopolymer matrices are summarized. Proof‐of‐concept in vitro/in vivo results are also discussed for oral delivery of these agents by various biopolymer vehicles, which can be found so far from the literature. Last but not the least, the challenges and new opportunities in the field are highlighted.

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Multispectroscopic, electrochemical and molecular docking approaches on binding comparison of camptothecin, 10-hydroxycamptothecin to bovine serum albumin

Cited by 28 publications

References 31 publications

Interactions between Two Kinds of Gold Nanoclusters and Calf Thymus Deoxyribonucleic Acid: Directions for Preparations to Applications

Interactions between Two Kinds of Gold Nanoclusters and Calf Thymus Deoxyribonucleic Acid: Directions for Preparations to Applications

Eu‐doped ZnAl‐LDH as a Fluorescent Labeling Carrier for the Delivery and Controlled Release of Camptothecin

Biopolymer Nanovehicles for Oral Delivery of Natural Anticancer Agents

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