2009
DOI: 10.1016/j.fertnstert.2008.05.046
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Multistep and single-step treatment of human spermatozoa with cryoprotectants

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Cited by 10 publications
(7 citation statements)
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“…The gradual application of permeant cryoprotectants at isotonicity is suggested to be less damaging to human and bovine spermatozoa than a single-step addition, by limiting the extent of cell membrane excursion, and thus cell damage, at each step [47][48][49]. However, gradual exposure to hypotonic media was found to be more damaging to rat spermatozoa than that caused during the single step and caused fewer caput cells to swell, although this could be an effect of the repeated centrifugation.…”
Section: Discussionmentioning
confidence: 97%
“…The gradual application of permeant cryoprotectants at isotonicity is suggested to be less damaging to human and bovine spermatozoa than a single-step addition, by limiting the extent of cell membrane excursion, and thus cell damage, at each step [47][48][49]. However, gradual exposure to hypotonic media was found to be more damaging to rat spermatozoa than that caused during the single step and caused fewer caput cells to swell, although this could be an effect of the repeated centrifugation.…”
Section: Discussionmentioning
confidence: 97%
“…In this context, it could be hypothesized that PDO could function as a CPA itself, thereby mitigating the potential cryodamage that one would have expected to observe as a consequence of the lower intracellular concentration of glycerol. In fact, Widiasih et al [55] observed that, when the impact of different cryoprotectants was evaluated, PDO yielded higher motility and viability in human spermatozoa than glycerol. Moreover, PDO has also been used as a CPA for the cryopreservation of canine ovarian cortex [56] and human multipotent stromal cells [57].…”
Section: Discussionmentioning
confidence: 99%
“…Concerning the GFE group, the addition of PDO seemed to better preserve the overall quality and function of sperm in terms of TMOT, PMOT, viability, and membrane lipid disorder, which might be a consequence of the expected cryoprotective effect. In this context, PDO has been used as a cryoprotective agent in cryopreservation protocols for canine ovarian cortices [39], and Widiasih et al [40] demonstrated that PDO has milder detrimental effects on human sperm quality than glycerol. Moreover, even if the increase in MMP does not have an apparent cause, it is also a good sperm quality indicator and it might be the cause of the higher percentage of viable spermatozoa with high levels of ROS, since mitochondria are the main source of these molecules.…”
Section: Discussionmentioning
confidence: 99%