Multistratified Expression of Polysialic Acid and Its Relationship to VAChT-containing Neurons in the Inner Plexiform Layer of Adult Rat Retina

Sawaguchi, Akira; Idate, Yoshiko; Ide, Soyuki; Kawano, Junichi; Nagaike, Ryoko; Oinuma, Tsutomu; Suganuma, Tatsuo

doi:10.1177/002215549904700709

Cited by 14 publications

(13 citation statements)

References 39 publications

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“…In the present study, PSA-NCAM immunoreactivity was demonstrated in control, sham-operated, and grafted retinas in the OPL and the IPL, which is in accordance with previous results reported by Sawaguchi et al (1999) demonstrating immunostaining in the IPL for PSA in a multistratified pattern. To our knowledge, TUC-4 expression has previously not been demonstrated in the adult retina.…”

Section: Extracellular Guidance Cuessupporting

confidence: 93%

“…PSA-NCAM is believed to be one of the factors guiding ganglion cell axon growth toward the optic disk (Brittis and Silver, 1995). PSA-NCAM expression is described in the plexiform layers of the adult retina as well as in regions of the adult brain, where migration of neuronal precursors occur (Sawaguchi et al, 1999;Kornack and Rakic, 2001;Seki, 2002). Furthermore, the TUC family of proteins (earlier referred to as TOAD, CRMP, Ulip, and DRP) has emerged as a strong candidate for a role in growth cone signaling (Quinn et al, 1999).…”

Section: Extracellular Guidance Cuesmentioning

confidence: 99%

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Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats

Wojciechowski

Englund

Lundberg

et al. 2004

Glia

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As previously reported, the brain-derived precursor cell line RN33B has a great capacity to migrate when transplanted to adult brain or retina. This cell line is immortalized with the SV40 large T-antigen and carries the reporter gene LacZ and the green fluorescent protein GFP. In the present study, the precursor cells were transplanted to the subretinal space of adult rats and investigated early after grafting. The purpose was to demonstrate the migration of the grafted cells from the subretinal space into the retina and the glial cell response of the host retina. Detachment caused by the transplantation method was persistent up to 4 days after transplantation, and then reattachment occurred. The grafted cells were shown to migrate in between the photoreceptor cells before entering into the plexiform layers. Molecules involved in migration of immature neuronal cells as the polysialylated neural cell adhesion molecule (PSA-NCAM) and the collapsing response-mediated protein 4 (TUC-4) was found in the plexiform layers of the host retina, but not in the grafted cells. The expression of the intermediate filaments GFAP, vimentin, and nestin was intensely upregulated immediately after transplantation. A less pronounced upregulation was observed on sham-operated animals. In summary, the RN33B cell line migrated promptly posttransplantation and settled preferably into the plexiform layers of the retina, the same layers where the migration cues PSA-NCAM and TUC-4 were established. In addition, both the transplantation method per se and the implanted cells caused an intense glial cell response by the host retina.

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Section: Extracellular Guidance Cuessupporting

confidence: 93%

Section: Extracellular Guidance Cuesmentioning

confidence: 99%

Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats

Wojciechowski

Englund

Lundberg

et al. 2004

Glia

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“…1A, 4A). Double-label experiments with Y1 and VAChT, which label the processes and terminals of cholinergic amacrine cells in strata 2 and 4 of the IPL (Sawaguchi et al, 1999), demonstrated the colocalization of Y1 immunoreactivity with VAChT ( Fig. 4A,B).…”

Section: Y1 Immunoreactivity Is Localized To Cholinergic Amacrine Cellsmentioning

confidence: 89%

“…Double-label experiments with cholinergic amacrine cell markers ChAT and VAChT showed that Y1 immunoreactivity in these strata was localized to cholinergic amacrine cell processes (Voigt, 1986;Sawaguchi et al, 1999).…”

Section: Cholinergic Amacrine Cells Express Y1mentioning

confidence: 99%

Localization of neuropeptide Y1 receptor immunoreactivity in the rat retina and the synaptic connectivity of Y1 immunoreactive cells

D'Angelo¹,

Oh²,

Chun³

et al. 2002

J of Comparative Neurology

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Neuropeptide Y (NPY), an inhibitory neuropeptide expressed by a moderately dense population of wide-field amacrine cells in the rat retina, acts through multiple (Y1-y6) G-protein-coupled receptors. This study determined the cellular localization of Y1 receptors and the synaptic connectivity of Y1 processes in the inner plexiform layer (IPL) of the rat retina. Specific Y1 immunoreactivity was localized to horizontal cell bodies in the distal inner nuclear layer and their processes in the outer plexiform layer. Immunoreactivity was also prominent in cell processes located in strata 2 and 4, and puncta in strata 4 and 5 of the IPL. Double-label immunohistochemical experiments with calbindin, a horizontal cell marker, confirmed Y1 immunostaining in all horizontal cells. Double-label immunohistochemical experiments, using antibodies to choline acetyltransferase and vesicular acetylcholine transporter to label cholinergic amacrine cell processes, demonstrated that Y1 immunoreactivity in strata 2 and 4 of the IPL was localized to cholinergic amacrine cell processes. Electron microscopic studies of the inner retina showed that Y1-immunostained amacrine cell processes and puncta received synaptic inputs from unlabeled amacrine cell processes (65.2%) and bipolar cell axon terminals (34.8%). Y1-immunoreactive amacrine cell processes most frequently formed synaptic outputs onto unlabeled amacrine cell processes (34.0%) and ganglion cell dendrites (54.1%). NPY immunoreactivity in the rat retina is distributed primarily to strata 1 and 5 of the IPL, and the present findings, thus, suggest that NPY acts in a paracrine manner on Y1 receptors to influence both horizontal and amacrine cells. Indexing termsamacrine cells; choline acetyltransferase; calcium binding protein; horizontal cells; neuropeptides Neuropeptide Y (NPY) has multiple physiologic actions in both the central and peripheral nervous system, including effects on blood flow, memory retention, food intake, epilepsy, and pain (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and Herzog, 1997;Thorsell et al., 2000;Furtinger et al., 2001;Naveilhan et al., 2001). These NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript effects are mediated by means of at least five G-protein-coupled receptors, designated as Y1, Y2, Y4, Y5, and y6, which are coupled to pertussis toxin-sensitive inhibitory Gproteins (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and Herzog, 1997;Michel et al., 1998). Y-receptors have been localized to specific regions of the central and peripheral nervous system by receptor binding autoradiography, immunohistochemistry, and in situ hybridization (Zhang et al., 1994;Bao et al., 1997;Jacques et al., 1997;Dumont et al., 1998;Hökfelt et al., 1998;Gackenheimer et al., 2001). Y1 receptor is the most studied Y-receptor and it plays key roles in many of the central and peripheral effects of NPY (Lundberg et al., 1996;Munglani et al., 1996;Balasubramaniam, 1997;Blomqvist and H...

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“…As mentioned previously by Bentrop et al [46], the ST8Sia III mRNA was also localized in the retina of the developing eye at 48 hpf and this expression is restricted to the inner cell layer of retina at the larval stage (5 dpf). Several sialylated glycoproteins such as PSA N-CAM or the sialoprotein associated with cones and rods (SPACR) expressed in the retina of fish [21,23,48], rodents [49,50] or birds [51] might be the acceptor substrates for the ST8Sia III activity. Finally, no expression of the zebrafish ST8Sia III gene could be detected in the trunk, which is in contrast to the observation made by Bentrop et al [46] of an intense expression of this gene between 20 hpf and 32 hpf in the fast muscle fibres of the entire myotome.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of the expression pattern of the zebrafish α2, 8-sialyltransferases (ST8Sia) in the developing nervous system

Chang

Mir²,

Thisse³

et al. 2008

Glycoconj J

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Sialyltransferases are Golgi type II transmembrane glycoproteins involved in the biosynthesis of sialylated glycolipids and glycoproteins. These sialylated compounds play fundamental roles in the development of a variety of tissues including the nervous system. In this study, we have molecularly cloned from zebrafish sources, the orthologues of the six human alpha2,8-sialyltransferases (ST8Sia), a family of sialyltransferases implicated in the alpha2-8-mono-, oligo-, and poly-sialylation of glycoproteins and gangliosides and we have analysed their expression pattern in the embryonic zebrafish nervous system, using in situ hybridization. Our results show that all six ST8Sia exhibit distinct and overlapping patterns of expression in the developing zebrafish central nervous system with spatial and temporal regulation of the expression of these genes, which suggests a role for the alpha2-8-sialylated compounds in the developing nervous system.

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Multistratified Expression of Polysialic Acid and Its Relationship to VAChT-containing Neurons in the Inner Plexiform Layer of Adult Rat Retina

Cited by 14 publications

References 39 publications

Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats

Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats

Localization of neuropeptide Y1 receptor immunoreactivity in the rat retina and the synaptic connectivity of Y1 immunoreactive cells

Molecular cloning and characterization of the expression pattern of the zebrafish α2, 8-sialyltransferases (ST8Sia) in the developing nervous system

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