Multivariate Analyses and Characterization of Volatile Components in Citrus Species

Abstract

The composition of volatile components in 18 Citrus Archicitrus species, 17 Citrus Metacitrus species and two Fortunella species, according to the classification system established by Tanaka, was investigated using headspace gas chromatography-mass spectrometry. The composition of 48 volatile compounds in the juice of these species was determined, and principal component analysis was performed. Scores for the 1st and 2nd principal components are plotted as a scatter diagram. In Citrus Archicitrus, samples of Citrophorum, Cephacitrus and Aurantium formed individual groups, but others did not. In Citrus Metacitrus, samples of Acrumen were divided into two different groups. The composition of volatile components in citrus juice was consistent with each grouping of Tanaka's system, in particular, in the case of dividing the Citrus genus into the two subgenera Archicitrus and Metacitrus.

Genomic Analysis of Terpene Synthase Family and Functional Characterization of Seven Sesquiterpene Synthases from Citrus sinensis

“…Besides, the hybrid nature of sweet orange [( C.maxima × C. reticulata ) × C. reticulate ], implies that diversification of gene families can also occur as a result of the combination of alleles from ancestral parents, as shown to happen for some nuclear genes studied (Garcia-Lor et al, 2013 ). In addition, ancestral parents can be distinguished based on their volatile profiles in different tissues (Yamamoto et al, 2013 ), thus advising that terpene metabolism is different between both species, and that sweet orange may have inherited TPS characters from both. As the genome analyzed in this work derives from a double-haploid C. sinensis callus line, allele's analysis was precluded.…”

Detection of Adulterated Shiikuwasha Juice by Sensory Evaluation, Colorimetric Value and Volatile Components

“…Solid phase microextraction (SPME)-GC, GC/MS for volatile components All analytical conditions and procedures were identical to those in a previous paper (Yamamoto et al, 2013). One milliliter of a juice sample was placed in a vial (22 × 38 mm) containing an internal standard solution of 10 μL of 1 % cyclohexanol in water.…”

Largely different contents of terpenoids in beef red-flesh tangerine and its wild type

“…Citrus plants are rich of terpenoids, and volatile organic compounds, phytohormones, carotenoids, limonoid aglycones are produced in leaves, flowers, roots and fruits. Monoterpenes and sesquiterpenes account for more than 80% of the total volatiles in citrus (Yamamoto et al [ 4 ]), and carotenoids (C 30 and C 40 ) and limonoid aglycones are the derivatives of tetraterpenoids and triterpenoids [ 4 ], while ABA is regarded as a sesquiterpene usually produced from the degradation of carotenoids [ 5 ]. Terpenoids in citrus play key roles in photosynthesis, plant growth regulation, plant-environment interactions and fruit quality.…”