Multivariate comparative analysis of chemical constituent changes and antioxidant properties of polysaccharides in ribes stenocarpum maxim. at different maturity stages on the Qinghai-Tibet Plateau

Liang, Xinyue; Ye, Ying; Zhu, Yunhe; Xiao, Jinrong; Qiao, Yangbo

doi:10.1016/j.scienta.2022.111556

Cited by 3 publications

(1 citation statement)

References 37 publications

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“…The Pearson correlation is a more commonly used method for calculating correlation coefficients. It is one of the most fundamental approaches for revealing the direction and degree of linear correlation between two variables, and this correlation analysis technique has been used to explore the structure and bioactivity relationships of polysaccharides in recent years [ 47 , 48 , 49 ]. The correlation between the monosaccharide composition, molecular weight and anti-complementary activity of CMPs was analyzed, and the results and visualizations are shown in Table 3 and Figure 10 .…”

Section: Resultsmentioning

confidence: 99%

Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Wang

Jin

et al. 2022

Polymers

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This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity. The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column. The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation. The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway. Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system. The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides. These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.

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Section: Resultsmentioning

confidence: 99%

Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Wang

Jin

et al. 2022

Polymers

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Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

Guo,

Zhao,

et al. 2024

Microscopy Res & Technique

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Rosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower is traditionally used in Kazak to treat the common cold, fever, and epileptic seizures and lessen the effects of aging. In the present study, the pharmacognostic profiles, physicochemical properties, phytochemical characteristics, and in vitro antioxidant potency of Rosa laxa Retz. flos (RLF) were presented. In the pharmacognostic evaluation of RLF, organoleptic characteristics, internal structures, and powder information were observed, and physicochemical parameters, including moisture content, ash, pH value, swelling degree, and extractives were examined. The quantitative analysis of the chemical composition of four different polar extracts of RLF showed that the aqueous part had the highest total triterpene acid, flavonoid, and polyphenol content (4.50 ± 0.04 mg/g, 50.56 ± 0.03 mg/g, and 60.20 ± 0.09 mg/g, respectively). A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. In the set concentration range, the linear relationship among the four components was good (r > 0.999), the average recoveries were 97.36%–100.54%. The contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF samples were (9.46 ± 2.31) mg/g, (10.60 ±0.75) mg/g, (1.13 ± 2.50) mg/g, and (1.11 ± 2.65) mg/g, respectively. The types of its secondary metabolites were determined by fluorescence, color reaction by chemical solvent method, and ultraviolet–visible (UV–Vis) spectroscopy. The functional groups of its secondary metabolites were determined by Fourier transform infrared (FTIR) spectroscopy. Results showed that RLF contains a variety of secondary metabolic products, including flavonoids, phenolic acid, glycoside, and organic acid. TLC identification showed it contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol‐3‐O‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of different polar parts of RLF was investigated by DPPH, ABTS, and reduction performance experiments. The aqueous extract had the strongest antioxidant capacity, consistent with the high content of triterpene acids, flavonoids, and polyphenolic compounds. These findings will provide critical information for the study of quality standards and medicinal value of RLF and its extracts, justify its usage in traditional medicinal systems, and encourage the use of this plant in disease prevention and treatment. Its phytochemical composition and pharmacological studies need to be explored in future.Research Highlights Optical microscope and scanning electron microscope (SEM) were used to observe the morphology, and microstructure of Rosa laxa Retz. flos (RLF). The physicochemical properties, fluorescence and phytochemical composition of four different polar extracts of RLF were analyzed by UV–Vis and FTIR. Determination of total triterpenic acid, total flavonoids, and total polyphenols in four different polar extracts of RLF by UV spectrophotometry. A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. TLC confirmed that RLF contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol 3‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of RLF was studied by DPPH, ABTS, and reducing ability experiments.

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Comparative study of bio-functional profile and bioactivities of polysaccharides from Ganoderma lucidum and Ganoderma neo-japonicum

Ayimbila,

Siriwong,

Chaiyama

et al. 2023

Biocatalysis and Agricultural Biotechnology

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Multivariate comparative analysis of chemical constituent changes and antioxidant properties of polysaccharides in ribes stenocarpum maxim. at different maturity stages on the Qinghai-Tibet Plateau

Cited by 3 publications

References 37 publications

Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris

Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

Comparative study of bio-functional profile and bioactivities of polysaccharides from Ganoderma lucidum and Ganoderma neo-japonicum

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