Multivariate modeling of chromium-induced oxidative stress and biochemical changes in plants of Pistia stratiotes L.

Abstract

Biochemical changes in the plants of Pistia stratiotes L., a free floating macrophyte exposed to different concentrations of hexavalent chromium (0, 10, 40, 60, 80 and 160 lM) for 48, 96 and 144 h were studied. Chromium-induced oxidative stress in macrophyte was investigated using the multivariate modeling approaches. Cluster analysis rendered two fairly distinct clusters (roots and shoots) of similar characteristics in terms of their biochemical responses. Discriminant analysis identified ascorbate peroxidase (APX) as discriminating variable between the root and shoot tissues. Principal components analysis results suggested that malondialdehyde (MDA), superoxide dismutase (SOD), APX, non-protein thiols (NP-SH), cysteine, ascorbic acid, and Cr-accumulation are dominant in root tissues, whereas, protein and guaiacol peroxidase (GPX) in shoots of the plant. Discriminant partial least squares analysis results further confirmed that MDA, SOD, NP-SH, cysteine, GPX, APX, ascorbic acid and Cr-accumulation dominated in the root tissues, while protein in the shoot. Three-way analysis helped in visualizing simultaneous influence of metal concentration and exposure duration on biochemical variables in plant tissues. The multivariate approaches, thus, allowed for the interpretation of the induced biochemical changes in the plant tissues exposed to chromium, which otherwise using the conventional approaches is difficult.

Effects of polycyclic aromatic hydrocarbons exposure on antioxidant system activities and proline content in Kandelia candel

“…Out results (with the data divided into three parts and 77.5% cases assigned correctly) suggested that all five parameters of K. candel under PAH-treatment showed a clear organ-specific feature. This finding is in agreement with the previous study of Pistia stratiotes L. under chromium stress (Sinha et al 2009). …”

“…With the two types of PAH treatments after two months, we measured the levels of antioxidant enzymes of SOD, CAT, POD and the contents of antioxidants of proline and MDA in a leaf, a stem and a root of mangrove plants of K. candel (a dominant mangrove species along the South China coast). The physiology of plants and the multiple factors of their cultivation environment are very dynamic (Landis et al 1994, Rodríguez-Ortega et al 2009), so a proper statistical analysis is necessary to explain the complex relationships and extract accurate information from the biological system (Sinha et al 2009). In this study we applied the analysis of variance (ANOVA), correlation analysis and discriminant analysis to investigate the responses of the antioxidant system of K. candel to PAH stress, as to identify the potential biomarkers for PAH contamination.…”

Estudo dos mecanismos de detoxificação e tolerância aos metais cromo e cobre em Pseudokirchneriella subcapitata e Pistia stratiotes e o uso das macrófitas Typha sp e Phragmites sp na remoção de nutrientes em wetlands construídos

“…Similar to our results, Sinha et al (2009) suggested a pattern of variation and difference in responses of root and shoot tissues of the plant in terms of dominance of the biochemical variables at all concentrations and durations.…”

“…A técnica forneceu informações de um padrão diferencial no início e na magnitude do estresse oxidativo induzido na raiz e parte aérea das plantas expostas em função da concentração de cromo e do tempo de exposição. Exemplos como este tem emergido no sentido de gerar modelos de análise e interpretação de grandes conjuntos de dados com inter-relações complexas entre as variáveis e também tem sido aplicado com sucesso em vários estudos de caso: ambientais, químicos, biológicos e ecotoxicológicos (SINHA et al, 2009;SINGH et al, 2004).…”

“…Poor translocation of chromium to the leaves could be due to sequesterization of most of the chromium in the vacuoles of the root cells to render in nontoxic which may be a natural toxicity response of the plant . Sinha et al (2009) reported that a metal concentration gradient, thus build up between the root and shoots tissue of the exposed plants may lead to difference in setting up and magnitude of various responses in these tissues. Thus, Sinha et al (2005) reported that the relation between Cr induced oxidative stress and capacity antioxidant in P. stratiotes suggest that the tolerance 184 capacity of the plants to the metal depends on the balance of the factors favouring oxidative stress and factors reducing oxidative stress.…”