Multivisceral transplantation in pigs: technical aspects

Alessiani, M.; Spada, Marco; Vaccarisi, S.; Fayer, F; Puletti, Giovanni; Ruggiero, R; Bellinzona, G; Noli, S; Scandone, M; Maestri, Marcello; Dionigi, Paolo; Zonta, A.

doi:10.1016/s0041-1345(98)00756-8

Cited by 6 publications

(15 citation statements)

References 3 publications

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“…Although the pathohistological changes are well known (Przepiorka et al, 1994;Alessiani et al, 1996a;Paulsen, 2000;Alessiani et al, 2003;Cahn et al, 2005), the exact immunological mechanism is not clear. Interestingly, however, the skin histopathological findings after Itx are similar to acute GVHD skin changes after bone marrow transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…Details of graft procurement, surgical procedures, tacrolimus-based immunosuppressive therapy and animal care have been reported previously (Alessiani et al, 1996a;Alessiani et al, 1996b;Gable et al, 1998;Kellersman et al, 2000). Orthotopic small intestinal transplantation (Starzl et al, 1992;Alessiani et al, 1996a;Alessiani et al, 2003) was performed.…”

Section: Methodsmentioning

confidence: 99%

“…Orthotopic small intestinal transplantation (Starzl et al, 1992;Alessiani et al, 1996a;Alessiani et al, 2003) was performed. The intestinal graft was composed with the proximal jejunum just after the ligament of Treitz and 20 cm proximal to the ileocaecal valve and the superior mesenteric artery and vein.…”

Section: Methodsmentioning

confidence: 99%

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Skin biopsies predict acute graft-versus-host disease after small bowel transplantation in pigs

Pintar

Pleskovič

Alessiani

et al. 2007

Acta Veterinaria Hungarica

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Intestinal transplantation is being increasingly performed to treat patients with irreversible intestinal failure. The major cause of intestinal graft failure is graft-versus-host disease (GVHD) that represents a life-threatening complication after small bowel transplantation (Itx). The purpose of this study was to assess the diagnostic and prognostic value of skin biopsy histological changes for acute GVHD after Itx in pigs. Thirty-four Large White pigs were divided into three groups: Group 1 with Itx only, Group 2 with Itx and donor bone marrow infusion (Itx BM) and Group 3 (control group -before the operation). Animals received tacrolimus-based immunosuppression from day 0 to day 30 postoperatively. Skin and small bowel biopsies were histologically assessed, analysed and classified from grade 1 to 4 on postoperative days 15, 30, 45 and 60. There was a strong correlation between the histological grading values of skin biopsy changes and the histological grading values of small bowel biopsy changes (Kendall's tau_b is 0.855 for the Itx group and 0.730 for the Itx BM group). The significant correlation found between skin and small bowel histological changes suggests the prognostic value of skin biopsies after Itx. In conclusion, our findings emphasise the diagnostic and prognostic value of skin biopsy analysis for acute GVHD after Itx.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Skin biopsies predict acute graft-versus-host disease after small bowel transplantation in pigs

Pintar

Pleskovič

Alessiani

et al. 2007

Acta Veterinaria Hungarica

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“…Some authors have described an additional resection of parts or the whole recipient's large bowel [8,15,20,26,28,[32][33][34]. In a few cases, the small and large bowel have been left completely in place or only small parts were resected [24,35,36] (Fig. 1).…”

Section: Recipient Native Enterectomy Before Sbtxmentioning

confidence: 99%

“…There exist a few models in which the portal vein of the donor and recipient was anastomosed. If the dSMV is used, it is usually anastomosed to the superior mesenteric vein (rSMV), the inferior vena cava or the iliac vein of the recipient [5,10,13,17,24,35]. The dSMV-rSMV anastomosis is performed end-to-end either with continuous 6-0 Prolene [4,28], 5/6-0 monofilament [21,35], or 7-0 PDS [14] sutures or interrupted 8-0 Prolene sutures [15].…”

Section: Implantation Of the Graft -Vascular Reconstruction -Venous Rmentioning

confidence: 99%