Murein hydrolase activity of surface layer proteins from Lactobacillus acidophilus against Escherichia coli

Meng, Jianghong; Gao, Shuming; Zhang, Qiuxiang; Lu, Rongrong

doi:10.1016/j.ijbiomac.2015.03.057

Cited by 24 publications

(18 citation statements)

References 27 publications

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“…Moreover, Meng et al demonstrated that L. acidophilus strains possessed murein hydrolase activities that are sublethal to E. coli cells [33]. Lipocalin-2 (Lcn2), a multifunctional innate immune protein derived from L. lactis inhibited the growth of E. coli and reduced the bioactivity of enterobactin (E. coli-derived siderophore) in vitro [34].…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm and Antiadhesive Activities of Different Synbiotics

Stefania¹,

Mir²,

Diana³

et al. 2017

J Prob Health

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Synbiotics are a combination of probiotic bacteria and the growth promoting prebiotic ingredients that purport "synergism".Probiotics are well known for their ability to restore a healthful balance of commensal microbes supporting the repair of hyper-permeable epithelial barriers and interfering with infection. The present research was designed to evaluate the anti-virulence role of cell-free supernatants of synbiotic cultures.The effect of prebiotics fructo-oligosaccharide, inulin and isomaltose on probiotic Lactobacillus acidophilus, Lactococcus lactis, Lactobacillus casei, Lactobacillus reuteri and Saccharomyces boulardii growth, fermentation products has been investigated. Cell-free supernatants of synbiotics cultures have been studied for their antimicrobial, antibiofilm and anti-adhesion properties. Results highlight that prebiotics can enhance the antimicrobial and anti-virulence activities of probiotics against potential pathogens Staphylococcus aureus and Escherichia coli.The present research supports, for the first time, the value of anti-virulence activity of metabolites produced by synbiotics suggesting their use as a suitable adjuvant in antibacterial treatment.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm and Antiadhesive Activities of Different Synbiotics

Stefania¹,

Mir²,

Diana³

et al. 2017

J Prob Health

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“…We propose, for biotechnological applications, that pre-growth of lactobacilli in high-salt conditions would result in an advantage for the probiotic nature of cells: the increased production and release of the S-layer might be appropriate for their antimicrobial capacity (Prado-Acosta et al 2008Martínez et al 2012;Meng et al 2015). Also, the reduction of the LTA content needs to be evaluated for possible anti-inflammatory (Kim et al 2014;Kaji et al 2010) or inflammatory effects (Lebeer et al 2012;Mohamadzadeh et al 2011;Zadeh et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356

Palomino

Waehner

Martin

et al. 2016

Appl Microbiol Biotechnol

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In this work, we studied the role of surface layer (S-layer) proteins in the adaptation of Lactobacillus acidophilus ATCC 4356 to the osmotic stress generated by high salt. The amounts of the predominant and the auxiliary S-layer proteins SlpA and SlpX were strongly influenced by the growth phase and high-salt conditions (0.6 M NaCl). Changes in gene expression were also observed as the mRNAs of the slpA and slpX genes increased related to the growth phase and presence of high salt. A growth stage-dependent modification on the S-layer protein profile in response to NaCl was observed: while in control conditions, the auxiliary SlpX protein represented less than 10 % of the total S-layer protein, in high-salt conditions, it increased to almost 40 % in the stationary phase. The increase in S-layer protein synthesis in the stress condition could be a consequence of or a way to counteract the fragility of the cell wall, since a decrease in the cell wall thickness and envelope components (peptidoglycan layer and lipoteichoic acid content) was observed in L. acidophilus when compared to a non-S-layer-producing species such as Lactobacillus casei. Also, the stationary phase and growth in high-salt medium resulted in increased release of S-layer proteins to the supernatant medium. Overall, these findings suggest that pre-growth in high-salt conditions would result in an advantage for the probiotic nature of L. acidophilus ATCC 4356 as the increased amount and release of the S-layer might be appropriate for its antimicrobial capacity.

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“…Murein hydrolases are able to hydrolyze bacterial CW components and cause cell death 35 ; this activity generally causes rapid lysis of bacterial cells. Previous studies have indicated that the S-layer protein SA of L. acidophilus causes CW peptidoglycan hydrolysis, therefore enhancing passage of nisin into the cell membrane by enabling it to cross the CW 16, 36 . This action caused the rapid lysis of Salmonella cells.…”

Section: Discussionmentioning

confidence: 99%

Synergistic antibacterial mechanism of the Lactobacillus crispatus surface layer protein and nisin on Staphylococcus saprophyticus

Sun

Liu

et al. 2017

Sci Rep

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SlpB, a surface layer protein isolated from Lactobacillus crispatus, has the potential to enhance the antimicrobial activity of nisin. Previous research indicated that, when combined with nisin, SlpB acted synergistically to inhibit Staphylococcus saprophyticus growth, thus extending the shelf life of chicken meat. In order to understand how SlpB enhances the antibacterial activity of nisin, electron microscopy, confocal laser scanning microscopy, flow cytometry and transmembrane electrical potential analysis were used to study cell wall organization and cell membrane integrity. No remarkable bacteriolytic effects were observed, indicating that cell death could not be attributed to cell lysis, although SlpB caused dramatic modifications of cell wall, thereby altering cell shape. The combination of SlpB and nisin also induced the release of ATP or UV-absorbing materials, as well as sudden dissipation of the transmembrane electrical potential by compromising membrane integrity. Considering that SlpB led to structural disorganization of the cell wall, and nisin access is enhanced to form a stable pore, cell death is a predictable outcome. SlpB significantly enhanced the effect of nisin at half of the minimum inhibitory concentration, which resulted in cell death by destroying the cell wall and cell membrane, therefore providing a new, feasible approach in food preservation.

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Murein hydrolase activity of surface layer proteins from Lactobacillus acidophilus against Escherichia coli

Cited by 24 publications

References 27 publications

Antibiofilm and Antiadhesive Activities of Different Synbiotics

Antibiofilm and Antiadhesive Activities of Different Synbiotics

Influence of osmotic stress on the profile and gene expression of surface layer proteins in Lactobacillus acidophilus ATCC 4356

Synergistic antibacterial mechanism of the Lactobacillus crispatus surface layer protein and nisin on Staphylococcus saprophyticus

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