Muricauda indica sp. nov., isolated from deep sea water

Abstract

A taxonomic study was carried out on strain 3PC125-7, which was isolated from the deep sea water of the Indian Ocean. The bacterium was rod-shaped, non-flagellated, Gram-stain-negative, oxidase- and catalase-positive and strictly aerobic. Optimal growth was observed at 25-37 °C, at pH 7 and in 1-3 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence analysis, strain 3PC125-7 represents a member of the genus Muricauda, with the highest sequence similarity to Muricauda olearia CL-SS4 (96.7 %), followed by Muricauda marina H19-56 (96.7 %) and nine other species of the genus Muricauda(93.5-95.8 %). The principal fatty acids of 3PC125-7 were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1G and the sole respiratory quinone was menaquinone-6. The polar lipids comprise phosphatidylethanolamine, six unidentified phospholipids and three unknown lipids. The genomic DNA G+C content of 3PC125-7 was 41.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data obtained in this study, strain 3PC125-7 is considered to represent a novel species in the genus Muricauda, for which the name Muricaudaindica sp. nov. is proposed, with the type strain 3PC125-7 (=MCCC 1A03198=KCTC 52318).

Muricauda hadalis sp. nov., a novel piezophile isolated from hadopelagic water of the Mariana Trench and reclassification of Muricauda antarctica as a later heterotypic synonym of Muricauda teanensis

“…lutimaris KCTC 22173 T (GenBank accession number QXFH00000000) were downloaded from the NCBI database. The genomic DNA G+C content of strain MT-229 T was 45.6 mol% according to its draft genome sequence, which was consistent with DNA G+C contents of 39.9–50.7 mol% in genus Muricauda [7, 9].…”

“…The genus Muricauda was proposed by Bruns et al [1]. At the time of writing, the genus Muricauda comprises 19 species with validly published names (http://www.bacterio.net/Muricauda.html [2]), most of which have been isolated from coastal and marine environments such as tidal flat sediments [3–5], marine sediments [1, 6, 7] and seawater [8–12]. Members of this genus are characterized as Gram-negative, with a large amount of isoC 15 : 0 , iso-C 17 : 0 3-OH and iso-C 15 : 0 G as major fatty acids and menaquinone MK-6 as the sole respiratory quinone [1, 3].…”

“…antarctica CGMCC 1.2174 T , M. taeanensis JCM 17757 T and Muricauda indica 3PC125-7 T , with a large amount of isoC 15 : 0 , iso-C 17 : 0 3-OH and iso-C 15 : 1 G [3, 9, 26].…”

Muricauda oceani sp. nov., isolated from the East Pacific Ocean

“…Cells of members of the genus Muricauda are characterized as Gram-stain-negative, rod-shaped, and facultatively anaerobic or strictly aerobic, chemotaxonomically with large amounts of branched-and straight-chain fatty acids, with menaquinone-6 (MK-6) as the major ubiquinone, and by DNA G+C contents between 41 and 51 mol%. Also, most of the species are characterized by cellular appendages [1,[3][4][5][6][7][8][9][10][11][12][13]. Herein, we describe a novel strain, designated strain 501str8 T , isolated from a sediment sample from the East Pacific Ocean.…”

“…Anaerobic growth was assessed on MA with an anaerobic incubator (YQX-T, Shanghai Xiren Instrument) at 25 °C for 2 weeks. The temperature range for growth was determined on MA by incubating cultures at 8-45 °C (8,16,25,28,32,37, 42, 45 °C) for 7 days, and at 0 and 4 °C on MA for 30 days. The NaCl concentration range and optimum for growth were determined in NaCl-free synthetic marine ZoBell broth (5 g peptone, 1 g yeast extract and 0.01 g FePO 4 in 1 l water) supplemented with various concentrations of NaCl (0-12.0 % w/v, at intervals of 0.5 units).…”