Muricauda oceani sp. nov., isolated from the East Pacific Ocean

Abstract

<jats:p>A Gram-stain-negative bacterium, designated strain 501str8<jats:sup>T</jats:sup>, was isolated from a sediment sample collected from the East Pacific Ocean. 16S rRNA gene sequence analysis revealed that strain 501str8<jats:sup>T</jats:sup> belonged to the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8170" xlink:type="simple">Muricauda</jats:ext-link> </jats:named-content> </jats:italic>, with closely related type strains <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9454" xlink:type="simple">Muricauda aquimarina</jats:ext-link> </jats:named-content> </jats:italic> SW-63<jats:sup>T</jats:sup> (98.5 %), <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13453" xlink:type="simple">Muricauda lutimaris</jats:ext-link> </jats:named-content> </jats:italic> SMK-108<jats:sup>T</jats:sup> (98.3 %) and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8171" xlink:type="simple">Muricauda ruestringensis</jats:ext-link> </jats:named-content> </jats:italic> B1<jats:sup>T</jats:sup> (97.9 %). Up-to-date bacterial core gene set analysis revealed that strain 501str8<jats:sup>T</jats:sup> represented one independent lineage with <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9454" xlink:type="simple">M. aquimarina</jats:ext-link> </jats:named-content> </jats:italic> SW-63<jats:sup>T</jats:sup>. The average nucleotide identity values of strain 501str8<jats:sup>T</jats:sup> with <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9454" xlink:type="simple">M. aquimarina</jats:ext-link> </jats:named-content> </jats:italic> SW-63<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13453" xlink:type="simple">M. lutimaris</jats:ext-link> </jats:named-content> </jats:italic> SMK-108<jats:sup>T</jats:sup> were 80.2 and 81.3 %, respectively. <jats:italic>In silico</jats:italic> DNA–DNA hybridization values between strain 501str8<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.9454" xlink:type="simple">M. aquimarina</jats:ext-link> </jats:named-content> </jats:italic> SW-63<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.13453" xlink:type="simple">M. lutimaris</jats:ext-link> </jats:named-content> </jats:italic> SMK-108<jats:sup>T</jats:sup> were 22.8 and 32.9 %, respectively. The predominant isoprenoid quinone was menaquinone-6, and iso-C<jats:sub>15 : 0</jats:sub>, iso-C<jats:sub>17 : 0</jats:sub> 3-OH and iso-C<jats:sub>15 : 1</jats:sub> G were the dominant cellular fatty acids. The G+C content of the genomic DNA was 42.8 mol%. Differential phylogenetic distinctiveness and chemotaxonomic differences, together with the phenotypic properties observed in this study, revealed that strain 501str8<jats:sup>T</jats:sup> could be differentiated from closely related species. Therefore, we propose that strain 501str8<jats:sup>T</jats:sup> represents a novel species of the genus <jats:italic> <jats:named-content content-type="genus"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.8170" xlink:type="simple">Muricauda</jats:ext-link> </jats:named-content> </jats:italic>, for which the name <jats:italic>Muricauda oceani</jats:italic> sp. nov. is suggested. The type strain is 501str8<jats:sup>T</jats:sup> (=JCM 33902<jats:sup>T</jats:sup>=MCCC 1K04567<jats:sup>T</jats:sup>).</jats:p>

Muricauda maritima sp. nov., Muricauda aequoris sp. nov. and Muricauda oceanensis sp. nov., three marine bacteria isolated from seawater

“…¶Data was taken from Bruns et al [1]. **Data was taken from Dong et al [17]. † †Data was taken from Zhang et al [18].…”

“…The following eight strains were used as reference strains for comparative experiments. Muricauda antarctica Ar-22 T was available from our lab, Muricauda ruestringensis DSM 13258 T was obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Muricauda taeanensis JCM 17757 T and Muricauda aquimarina JCM 11811 T were obtained from the Japan Collection of Microorganisms, Muricauda beolgyonensis KCTC 23501 T and Muricauda lutimaris KCTC 22173 T were obtained from the Korean Collection for Type Culture, and Muricauda oceani 501str8 T and Muricauda hadalis MT-229 T were obtained from Chen's [17] and Fang’s labs [18], respectively. All strains were cultured in marine broth 2216 (MB; BD) or on MA at 30 °C and preserved at −80 °C with 25% (v/v) glycerol for long-term storage.…”

“…oceani 501str8 T (Fig. S3) [17], which were not present in strain 40DY170 T . The three strains could also be distinguished from the type strains of the Muricauda species by different phenotypic characteristics such as growth temperature, pH, NaCl range and optimum, hydrolysis of starch, enzyme activities, carbohydrate utilization, acid production, and sensitivity to antibiotics ( and S1).…”

Croceivirga litoralis sp. nov., isolated from coastal surface water, and reclassification of Muricauda lutea as Croceivirga lutea comb. nov.

“…Different from the G+C content (50.3 mol%) determined by HPLC [5], the G+C content of M. lutea MCCC 1K03195 T calculated from the draft genome sequence was 35.0 %. Compared to the genomic G+C contents of the genus Muricauda (ranging from 41 to 51 mol%) [23], the G+C content of M. lutea MCCC 1K03195 T is closer to those of ASW18 T (37.3 %) and C. radicis MCCC 1A06690 T (37.1 %). The ANI, isDDH and AAI values between strain ASW18 T and C. radicis MCCC 1A06690 T were 79.6%, 23.1% and 82.4 %; between strain ASW18 T and M. lutea MCCC 1K03195 T were 73.1 %, 19.9% and 75.3 %; and between C. radicis MCCC 1A06690 T and M. lutea MCCC 1K03195 T were 72.2 %, 18.4% and 74.8 %.…”

“…lutea MCCC 1K03195 T calculated from the draft genome sequence was 35.0 %. Compared to the genomic G+C contents of the genus Muricauda (ranging from 41 to 51 mol%) [23], the G+C content of M. lutea MCCC 1K03195 T is closer to those of ASW18 T (37.3 %) and C.…”