Murine epidermal Langerhans cells and splenic dendritic cells present tumor‐associated antigens to primed T cells

Abstract

We examined the ability of epidermal Langerhans cells and splenic dendritic cells to present tumor-associated antigens (Ag) to immune T cells. Methylcholanthrene (MCA)-induced subcutaneous fibrosarcomas derived from C57BL/6 mice were used as tumor models. Our data demonstrate that both murine Langerhans cells and splenic dendritic cells have the capacity to present tumor-associated Ag to primed T cells. We found that variously treated tumor preparations (irradiated viable tumor cells, irradiated frozen-stored tumor cells, mitomycin C-treated viable tumor cells, and snap freeze-thawed tumor cell lysates) can be utilized for tumor Ag-pulsing. Primed CD4+ T cells demonstrated in vitro specificity towards their respective tumors and did not cross-react to other syngeneic MCA-induced or non-MCA-induced tumors. The T cell proliferative response critically depended on the presence of immune CD4+ T cells. We discuss the implications of these findings for the adoptive immunotherapy of cancer using immune CD4+ T cells.

Retroviral interleukin-7 gene transfer into human dendritic cells enhances T cell activation

“…Presentation of foreign antigens on the surface of DC can be achieved by antigen (peptide) pulsing. [14][15][16][17][18][19][20] According to recent observations, 21 exogenous peptides can enter the MHC class I pathway of antigen presentation and induce cytotoxic T cell responses. Alternatively, presentation of TAA in APC can be achieved by gene transfer techniques.…”

Histomorphometric Analysis of Age-Related Changes in Epithelial Thickness and Langerhans Cell Density of the Human Tongue.

“…Recently, Cohen et al (1994) demonstrated that murine LCs had the capacity to present tumorassociated antigen to primed T cells. In human skin cancer, the number of T6-positive LC per mm2 of section has been reported to decrease significantly (Meissner et al 1986).…”

Dendritic Cell Vaccine and its Application in Cancer Therapy

“…Tumor lysate is other approach for antigen loading of DCs. This approach has been used successfully in many studies [18][19][20][21]. Total antigen loading approaches have the potency to deliver self-antigens and immunosuppressive components, and ultimately reduce antitumor immune responses.…”