Murine Model of Wound Healing

Dunn, Louise; Prosser, Hamish; Tan, Joanne; Vanags, Laura Z.; Ng, M.; Bursill, Christina A.

doi:10.3791/50265

Cited by 157 publications

(144 citation statements)

References 12 publications

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“…Long-term (23 DPA) exposure to 10 µM mubritinib induced contraction of the wound epidermis, similar to that observed in mice after injury (Dunn et al, 2013), in contrast to the minimal wound contraction observed in control limbs (Fig. 3K,L).…”

Section: Nrg1 Supplementation Rescues Regeneration In Denervated Limbssupporting

confidence: 81%

Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration

et al. 2016

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The Mexican axolotl (Ambystoma mexicanum) is capable of fully regenerating amputated limbs, but denervation of the limb inhibits the formation of the post-injury proliferative mass called the blastema. The molecular basis behind this phenomenon remains poorly understood, but previous studies have suggested that nerves support regeneration via the secretion of essential growthpromoting factors. An essential nerve-derived factor must be found in the blastema, capable of rescuing regeneration in denervated limbs, and its inhibition must prevent regeneration. Here, we show that the neuronally secreted protein Neuregulin-1 (NRG1) fulfills all these criteria in the axolotl. Immunohistochemistry and in situ hybridization of NRG1 and its active receptor ErbB2 revealed that they are expressed in regenerating blastemas but lost upon denervation. NRG1 was localized to the wound epithelium prior to blastema formation and was later strongly expressed in proliferating blastemal cells. Supplementation by implantation of NRG1-soaked beads rescued regeneration to digits in denervated limbs, and pharmacological inhibition of NRG1 signaling reduced cell proliferation, blocked blastema formation and induced aberrant collagen deposition in fully innervated limbs. Taken together, our results show that nerve-dependent NRG1/ErbB2 signaling promotes blastemal proliferation in the regenerating limb and may play an essential role in blastema formation, thus providing insight into the longstanding question of why nerves are required for axolotl limb regeneration.

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Section: Nrg1 Supplementation Rescues Regeneration In Denervated Limbssupporting

confidence: 81%

Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration

et al. 2016

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“…However, wound healing in rodent models is fundamentally different to that in humans. This is because the major mechanism of wound closure is contraction, whereas in humans re-epithelialization and granulation tissue formation are the main means of healing (6,7). In addition, the porcine cutaneous wound healing model is frequently used as a model for human cutaneous wound healing (8).…”

Section: Animal Models Of Wound Healingmentioning

confidence: 99%

Models of wound healing: an emphasis on clinical studies

Wilhelm

Wilhelm²,

Bielfeldt³

2016

Skin Research and Technology

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Background: The healing of wounds has always provided challenges for the medical community whether chronic or acute. Understanding the processes which enable wounds to heal is primarily carried out by the use of models, in vitro, animal and human. It is generally accepted that the use of human models offers the best opportunity to understand the factors that influence wound healing as well as to evaluate efficacy of treatments applied to wounds. Objectives: The objective of this article is to provide an overview of the different methodologies that are currently used to experimentally induce wounds of various depths in human volunteers and examines the information that may be gained from them. Methods: There is a number of human volunteer healing models available varying in their invasiveness to reflect the different possible depth levels of wounds.Results: Currently available wound healing models include sequential tape stripping, suction blister, abrasion, laser, dermatome, and biopsy techniques. The various techniques can be utilized to induce wounds of variable depth, from removing solely the stratum corneum barrier, the epidermis to even splitthickness or full thickness wounds. Conclusion: Depending on the study objective, a number of models exist to study wound healing in humans. These models provide efficient and reliable results to evaluate treatment modalities. I N VITRO studies and animal models have provided useful information for many breakthroughs and medical advances. However, these are not always appropriate and in many cases are inadequate for discovering the actual pathology responsible. While animal models are suitable for many skin disorders and diseases, they do have their limitations when wound healing is being investigated. The complex biological pathway that occurs during wound healing is not always mirrored in animal models and human models need to be considered. In the case of chronic wound pathology, human models are not always good predictors but they do provide a good understanding of acute wound pathology. Because of the difficulty in obtaining suitable volunteers with chronic wounds and incorporating them into a study, human acute wound models provide the best opportunity to understand the wound healing process and the performance of different products that assist and promote wound healing. This paper evaluates the different wound healing models and discusses the potential for these models to be suitable predictors of wound healing.

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“…The use of experimental animals is highly crucial biological tools in human and veterinary medicine and it seems to remain reliable option as it was. Rodents are mostly chosen as experimental animals for acute and chronic wound model because their care and feeding are easy, they reproduce and grow quickly and their biological characteristics are well understood [3][4][5]11,12] . On the other hand, they create a significant problem during experimental studies in wound healing due to a loose connection between skin and subcutaneous tissues in these highly thin-skinned animals.…”

Section: Discussionmentioning

confidence: 99%

“…In human and veterinary medicine, experimental studies on animal models are widely used as an important biological approach to clarify the pathophysiology of wound healing and to identify new strategies [3,4] . Even if different animal species are used for this purpose, mice and other rodents are extensively preferred because their care and feeding are easy, they reproduce and grow quickly, suitable for application of surgical procedures and their biology is thoroughly investigated [3,5] . On the other hand, an excessive skin contraction is a reality and edges of the wound move rapidly towards the centre.…”

Section: Introductionmentioning

confidence: 99%

Tam Katmanlı Deri Eksizyonu Oluşturulmuş Diyabetik ve Non-Diyabetik Farelerde Yara Kontraksiyonunun Önlenmesi İçin Üç Farklı Fiksasyon Yönteminin Karşılaştırılması

Anuk¹,

Öztürk²,

Özaydin³

et al. 2016

Kafkas Univ Vet Fak Derg

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The most crucial problem in experimentally induced excisional wounds in animals with slim-skinned and poor subcutaneous attachment is wound contraction formed during recovery period. The contraction is mainly induced by myofibroblasts leading to early wound closure. This may be associated with false outcomes of the study. Recently, various trials have been made to reduce the contraction in full-thickness excisional models of wounds. This study aimed at comparing different fixation methods on the minimization of wound contraction. Study was carried out in two main groups as non-diabetic (Group I, n=24) and diabetic (Group II, n=24) and one control group. The animals in each group were divided into four equal subgroups (Group I-A, I-B, I-C, I-D; Group II-A, II-B, II-C, II-D) and 1.2x1.2 cm-sized full-thickness of skin excisions were generated on the dorsum of animals. No fixations were applied in subgroups I-A and II-A however, interrupted suture in subgroups I-B and II-B, suture passing from a couple of points through skin and subcutaneous tissues placed in each side of wound tied only in corners in subgroups I-C and II-C and using a polyethylene fixatives that fixed in wound edges with interrupted sutures in subgroups I-D and II-D were achieved to produce wound fixation methods. Digital photographs of the wound were taken on the 3 rd , 7th , 14 th and 21 st days of the study, reduction ratios of the wounds were compared and the efficiency of methods for the prevention of contraction was examined. When wounds were evaluated comparatively after fixation, it was observed that three different applied fixation methods were efficient in prevention of wound contraction in all phases in comparison to control groups. Moreover, it was observed that contraction was highly impeded in interrupted suture groups (I-B, II-B) and polyethylene-reinforced interrupted suture groups (I-D, II-D) when compared to suture groups tied only in corners. The results revealed that wound surface area shrinkage should be taken into consideration in experimental studies to avoid misleading consequences.

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Murine Model of Wound Healing

Cited by 157 publications

References 12 publications

Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration

Neuregulin-1 signaling is essential for nerve-dependent axolotl limb regeneration

Models of wound healing: an emphasis on clinical studies

Tam Katmanlı Deri Eksizyonu Oluşturulmuş Diyabetik ve Non-Diyabetik Farelerde Yara Kontraksiyonunun Önlenmesi İçin Üç Farklı Fiksasyon Yönteminin Karşılaştırılması

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