Murine Models of Human Wound Healing

Abstract

In vivo wound healing experiments remain the most predictive models for studying human wound healing, allowing an accurate representation of the complete wound healing environment including various cell types, environmental cues, and paracrine interactions. Small animals are economical, easy to maintain, and allow researchers to take advantage of the numerous transgenic strains that have been developed to investigate the specific mechanisms involved in wound healing and regeneration. Here we describe three reproducible murine wound healing models that recapitulate the human wound healing process.

Topical application of quercetin improves wound healing in pressure ulcer lesions

“…Animal experiments were performed according to the guidelines published by the Institute of Laboratory Animal Resources of National Research Council, and animal care for this study was approved by the Institutional Animal Care and Use Committee of Cangzhou Central Hospital. Pressure ulcers model was established following the procedure described before . Briefly, C57BL/6 mice were anesthetized with isoflurane inhalation.…”

Delivery of Mesenchymal Stem Cells in Biomimetic Engineered Scaffolds Promotes Healing of Diabetic Ulcers

“…The splinted excisional wound model was created as previously described [29]. In brief, diabetic mice were anesthetized and removed of hair over their back using a chemical depilatory.…”

Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing

“…cHG was synthesized using previously described methods. 8 , 15 Briefly, finger flexor tendons were harvested from fresh frozen cadaveric donors, decellularized, lyophilized, ground into a fine powder, and stored at −80°C. Samples were enzymatically digested in a 1 mg/mL solution of pepsin in hydrocloric acid to a final concentration of 25 mg/mL (2.5%) of tendon powder.…”

“…Four 10-mm circular wounds were created on the dorsum of each rat following a previously established model. 15 The wounds were stented open using a 15-mm silicone ring and securely attached using sutures. Two wounds per rat were treated with occlusive dressing, and 2 wounds were treated with occlusive dressing plus 100 μL of cHG seeded with 2 M/mL ADSCs tagged with luciferase (Fig.…”