Muropeptides trigger distinct activation profiles in macrophages and dendritic cells

Pashenkov, Мikhail; Popilyuk, S F; Алхазова, Б И; Львов, В Л; Муругин, В В; Феденко, Е С; Хаитов, Р. М.; Пинегин, Б В

doi:10.1016/j.intimp.2010.04.025

Cited by 12 publications

(16 citation statements)

References 25 publications

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“…As shown in Fig. 2A, the two cell types expressed similar levels of NOD1 and TLR4 mRNA, which agrees with our earlier data (14), so it is unlikely that the low response of MDDC to GM-triDAP is caused by low NOD1 expression.…”

Section: The Poor Tnf Secretion By Gm-tridap-stimulated Mddc Is Likelsupporting

confidence: 91%

“…GM-triDAP, prepared from peptidoglycan of Salmonella typhi as described (14), was a gift of V. L'vov (Institute of Immunology, Moscow, Russia). By limulus amebocyte lysate test (EndoSafe KTA; Charles River Laboratories, Wilmington, MA), endotoxin level in the GM-triDAP preparation was ,0.01 endotoxin unit/mg.…”

Section: Chemicals and Absmentioning

confidence: 99%

“…Human immature MDDC were generated as described (14,33) by culturing blood monocytes in RPMI 1640 (PAA Laboratories, Pasching, Austria) supplemented with 2 mM L-glutamine (Sigma-Aldrich), 10% FCS (PAA Laboratories), 80 ng/ml rhGM-CSF, and 50 ng/ml rhIL-4 for 6 d. MDM were generated using a similar procedure, except that rhIL-4 was omitted (14). In kinetic experiments, paired cultures of MDM and MDDC from the same donor were used.…”

Section: Culture and Stimulation Of Mddc And Mdmmentioning

confidence: 99%

“…NOD1 and NOD2, by means of highly similar signaling pathways (5), can trigger a variety of biological responses, including the production of inflammatory mediators, antimicrobial peptides, and type I IFNs, as well as autophagy and insulin resistance (6)(7)(8)(9)(10)(11)(12)(13). A central effect of NOD1 and NOD2 activation in myeloid cells is the induction of proinflammatory cytokines, such as TNF (9,12,14).…”

mentioning

confidence: 99%

“…Intriguingly, different types of myeloid cells, such as monocytes, macrophages, and myeloid dendritic cells, respond to muropeptides with very different magnitudes in terms of cytokine production (9,12,14,31). For example, when stimulated with NOD1 or NOD2 Laboratory of Clinical Immunology, National Research Center Institute of Immunology of the Federal Medical-Biological Agency, 115478 Moscow, Russia agonists for 24 h, monocyte-derived dendritic cells (MDDC) produce one to two orders of magnitude less TNF than do monocyte-derived macrophages (MDM), despite being generated from the same precursor and expressing similar levels of NOD1 and NOD2 (14). We therefore examined different stages of NODdriven TNF production in MDDC and MDM to determine critical factors regulating myeloid cell responses to muropeptides.…”

mentioning

confidence: 99%

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The Role of the p38–MNK–eIF4E Signaling Axis in TNF Production Downstream of the NOD1 Receptor

Pashenkov

Balyasova

Dagil

et al. 2017

The Journal of Immunology

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Activation of nucleotide-binding oligomerization domain (NOD) 1 and NOD2 by muropeptides triggers a complex transcriptional program in innate immune cells. However, little is known about posttranscriptional regulation of NOD1- and NOD2-dependent responses. When stimulated with a prototypic NOD1 agonist, N-acetylglucosaminyl-N-acetylmuramyl-l-alanyl-d-isoglutamyl-meso-diaminopimelic acid (GM-triDAP), human monocyte-derived macrophages (MDM) produced an order of magnitude more TNF, IL-6, and pro-IL-1β than did monocyte-derived dendritic cells (MDDC), despite similar NOD1 expression, similar cytokine mRNA kinetics, and comparable responses to LPS. TNF production by GM-triDAP-activated MDM was independent of autocrine IL-1. However, GM-triDAP-activated MDM translated TNF mRNA more efficiently than did MDDC. As an underlying mechanism, NOD1 triggering in MDM caused a more potent and long-lasting activation of the signaling axis involving p38 MAPK, MAPK-interacting kinase (MNK), and eukaryotic translation initiation factor 4E, which is a critical regulator of translation. Furthermore, MNK controlled TNF mRNA abundance in MDDC and MDM upon NOD1 triggering. NOD1-dependent responses were more sensitive to MNK inhibition than were TLR4-dependent responses. These results demonstrate the importance of the p38-MNK-eukaryotic translation initiation factor 4E axis in TNF production downstream of NOD1.

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Section: The Poor Tnf Secretion By Gm-tridap-stimulated Mddc Is Likelsupporting

confidence: 91%

Section: Chemicals and Absmentioning

confidence: 99%

Section: Culture and Stimulation Of Mddc And Mdmmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

The Role of the p38–MNK–eIF4E Signaling Axis in TNF Production Downstream of the NOD1 Receptor

Pashenkov

Balyasova

Dagil

et al. 2017

The Journal of Immunology

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Synergistic interactions between NOD receptors and TLRs: Mechanisms and clinical implications

Pashenkov

Murugina

Будихина

et al. 2018

Journal of Leukocyte Biology

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Interactions between pattern recognition receptors (PRRs) shape innate immune responses to particular classes of pathogens. Here, we review interactions between TLRs and nucleotide‐binding oligomerization domain 1 and 2 (NOD1 and NOD2) receptors, two major groups of PRRs involved in innate recognition of bacteria. Most of experimental data both in vitro and in vivo suggest that NODs and TLRs synergize with each other at inducing the production of cytokines and antimicrobial peptides. Molecular mechanisms of this synergy remain poorly understood, although several scenarios can be proposed: (i) direct interactions of signaling pathways downstream of NODs and TLRs; (ii) mutual transcriptional regulation of unique components of NOD‐dependent and TLR‐dependent signaling pathways; and (iii) interactions at the post‐transcriptional level. Potential practical implications of NOD‐TLR synergy are dual. In sepsis, where synergistic effects probably contribute to excessive proinflammatory cytokine production, blockade of NOD1, and/or NOD2 in addition to TLR4 blockade may be required to achieve therapeutic benefit. On the other hand, synergistic combinations of relatively small doses of NOD and TLR agonists administered before infection could be used to boost innate resistance against bacterial pathogens.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

Harvey

2014

Mass Spectrometry Reviews

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This review is the sixth update of the original article published in 1999 on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2010. General aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, arrays and fragmentation are covered in the first part of the review and applications to various structural typed constitutes the remainder. The main groups of compound that are discussed in this section are oligo and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals. Many of these applications are presented in tabular form. Also discussed are medical and industrial applications of the technique, studies of enzyme reactions and applications to chemical synthesis.

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Muropeptides trigger distinct activation profiles in macrophages and dendritic cells

Cited by 12 publications

References 25 publications

The Role of the p38–MNK–eIF4E Signaling Axis in TNF Production Downstream of the NOD1 Receptor

The Role of the p38–MNK–eIF4E Signaling Axis in TNF Production Downstream of the NOD1 Receptor

Synergistic interactions between NOD receptors and TLRs: Mechanisms and clinical implications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2009–2010

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