Muscarinic signalling affects intracellular calcium concentration during the first cell cycle of sea urchin embryos

Harrison, Patrick K.; Falugi, Carla; Angelini, Cristiano; Whitaker, Michael

doi:10.1016/s0143-4160(02)00057-x

Cited by 25 publications

(31 citation statements)

References 28 publications

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“…10 -3 M of any of these insecticides totally arrested development. This results depend on the fact that no cholinergic molecules are involved in fertilisation, as we demonstrated successively (Harrison et al, 2002). Thus, the high dose (that is about IC50, according to the previously shown data of Rakonczay) may cause a general toxicity effect, not related to cholinergic molecules.…”

Section: Invertebrate Models: the Sea Urchin Paracentrotus Lividussupporting

confidence: 56%

“…Y axis represents fluorescence (scale units = 0,2) units; X axis represents time (scale units 30 sec). These experiments were performed by Dr Harrison P. in the laboratory of Prof. Whitaker, MJ, and published in the paper Harrison et al, 2002. Actually, from the zygote stage, stimulation of ACh receptors may evoke calcium spikes, anticipating those related to the nuclear breakdown (Harrison et al, 2002). In this event, muscarinic drugs were proved to have a prominent role.).…”

Section: Invertebrate Models: the Sea Urchin Paracentrotus Lividusmentioning

confidence: 99%

See 1 more Smart Citation

Cholinergic Pesticides

Falugi¹,

Rakonczay²,

Thielecke³

et al. 2011

Pesticides - The Impacts of Pesticides Exposure

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Section: Invertebrate Models: the Sea Urchin Paracentrotus Lividussupporting

confidence: 56%

Section: Invertebrate Models: the Sea Urchin Paracentrotus Lividusmentioning

confidence: 99%

Cholinergic Pesticides

Falugi¹,

Rakonczay²,

Thielecke³

et al. 2011

Pesticides - The Impacts of Pesticides Exposure

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“…Recently, the data about ineffectiveness of ACh in the egg activation was confirmed by Harrison et al (2002) who demonstrated that neither nicotinic nor muscarinic receptors are involved in the intracellular [Ca 21 ] increase responsible for the fast block to polyspermy.…”

Section: Biogenic Aminesmentioning

confidence: 97%

“…As stated above, no cholinergic molecules are involved in Ca 21 release of the fast block to polyspermy (Harrison et al, 2002), but it is known that IP3 can by itself activate the egg (Crossley et al, 1978) without ion passage from the outer environment. Kuo et al (2000) reported that this Ca 21 rise, which required for egg activation and the subsequent events of development in eggs of all species, may be activated by intracytoplasmic release of NO, synthesized by NO synthase (NOS).…”

Section: Fast Block To Polyspermymentioning

confidence: 99%

“…The existence of a response to ACh and cholinomimetic drugs in sea urchin eggs and zygotes was investigated in P. lividus and Lytechinus pictus (Harrison et al, 2002). The Ca 21 -sensitive fluorescent probe, Fura-2 dextran, was employed to investigate the regulation of cytosolic-free Ca 21 concentration ([Ca 21 ]i) by cholinomimetic drugs in unfertilized and fertilized eggs of both the sea urchin species.…”

Section: Zygote and First Cleavagementioning

confidence: 99%

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Sea urchin development: An alternative model for mechanistic understanding of neurodevelopment and neurotoxicity

Falugi

Lammerding–Köppel

Aluigi

2008

Birth Defects Research Pt C

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Echinoderm early developmental stages might supply a good tool for toxicity testing in different fields, ranging from environment to food contamination, and in full respect of the 3Rs objectives (reduction, refinement, and replacement of animal experiments) that will eventually lead to the replacement of high vertebrate animal testing in toxicology. Sea urchin is one of the few organismic models considered by the European Agency for Alternative models. Actually, sea urchin embryonic development has been studied for over a century, and the complex nets of intercellular communications leading to the different events are well known, as well the possibility for environmental molecules and their residuals to interfere with such communications, causing developmental anomalies. In particular, the main goal of toxicologists since several years has been to establish a correlation between the cell-to-cell communications occurring during different developmental events and the signals occurring during neurogenesis, with the aim to pursue a mechanistic understanding of these processes and their deviations caused by stressors from different sources.

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The histamine H1 receptor activates the nitric oxide pathway at fertilization

Leguía

Wessel

2006

Molecular Reproduction Devel

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Sperm fusion with the egg initiates a signaling cascade that releases intracellular calcium (Ca(i) (2+)) from the endoplasmic reticulum (ER). In sea urchins, Ca(2+) is released as a single, large transient via two distinct pathways. The first depends on inositol 1,4,5-triphosphate (IP(3)) production and triggers the initial phase of Ca(2+) release, while the second depends on nitric oxide (NO) production and is thought to maintain the duration of the Ca(2+) wave. We identified a sea urchin homolog of the seven trans-membrane G protein-coupled receptor for histamine (suH(1)R) on the egg cell surface that activates NO production. Treatment with histamine (HA) causes fluctuations in the resting levels of NO in the egg, while antagonists or antibodies of H(1)R inhibit the rise of NO normally observed at fertilization. Inhibition of suH(1)R function decreases the maintenance, but not the amplitude, of the Ca(2+) transient and suggests that it is an integral part of the overall pathway leading to egg activation at fertilization in sea urchins.

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Muscarinic signalling affects intracellular calcium concentration during the first cell cycle of sea urchin embryos

Cited by 25 publications

References 28 publications

Cholinergic Pesticides

Cholinergic Pesticides

Sea urchin development: An alternative model for mechanistic understanding of neurodevelopment and neurotoxicity

The histamine H1 receptor activates the nitric oxide pathway at fertilization

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