Muscle fibre characteristics and metabolic response at slaughter in pigs of different halothane genotypes and their relation to meat quality

Essén‐Gustavsson, Birgitta; Karlström, K.; Lundström, Kerstin

doi:10.1016/0309-1740(92)90067-e

Cited by 80 publications

(38 citation statements)

References 21 publications

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“…A similar, although not identical, pattern of fiber type distribution was described in the PSE (Pale, Soft, and Exudative) condition of skeletal muscle at slaughter of stress susceptible pigs (Fazarinc et al, 1995). However, evidences of PSE meat [characterized by reduced water holding capacity, and marked in drip loss after slaughter (Essén-Gustavsson et al, 1992) were not observed in the present study]. The high number of types I and IIA fibers and the low number of IIB fibers seen in these samples could be genetic and/or related to pre-or post-natal growth (Lefaucheur et al, 2004).…”

Section: Spatial Distribution Pattern Of Myofiber Typescontrasting

confidence: 81%

Coordinated expression of myosin heavy chains, metabolic enzymes, and morphological features of porcine skeletal muscle fiber types

Quiroz-Rothe

Rivero

2004

Microscopy Res & Technique

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Combined methodologies of electrophoresis, immunoblots, immunohistochemistry, histochemistry, and photometric image analysis were applied to characterize porcine skeletal muscle fibers according to their myosin heavy chain (MyHC) composition, and to determine on a fiber-to-fiber basis the correlation between contractile [MyHC (s), myofibrillar ATPase (mATPase), and sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) isoforms], metabolic [succinate dehydrogenase (SDH), and glycerol-3-phosphate dehydrogenase (GPDH) activities, glycogen, and phospholamban (PLB) contents], and morphological [cross-sectional area (CSA), capillary, and nuclear densities] features of individual myofibers. An accurate delineation of MyHC-based fiber types was obtained with the immunohistochemical method developed. This protocol showed a high sensitivity and objectivity to delineate hybrid fibers with overwhelming dominance of one MyHC isoform. The phenotypic differences in contractile, metabolic, and morphological properties seen between fiber types were related with MyHC content. Slow fibers had the lowest mATPase activity (related to shortening velocity), the highest SDH activity (oxidative capacity), the lowest GPDH activity (glycolytic metabolism), and glycogen content, the smallest CSA, the greatest capillary, and nuclear densities, and expressed slow SERCA isoform and PLB, but not the fast SERCA isoform. The reverse pattern was true for pure IIB fibers, whereas type IIA and IIX fibers had intermediate properties. Hybrid fibers had mean values intermediate in-between their respective pure phenotypes. Discrimination of myofibers according to their MyHC content was possible on the basis of their contractile and non-contractile profiles. These intrafiber interrelationships suggest that myofibers of control pigs exhibit a high degree of co-ordination in their physiological, biochemical, and anatomical features. This study may well be a useful baseline for future work on the pig meat industry and also offers new prospects for muscle fiber typing in porcine experimental studies.

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Section: Spatial Distribution Pattern Of Myofiber Typescontrasting

confidence: 81%

Coordinated expression of myosin heavy chains, metabolic enzymes, and morphological features of porcine skeletal muscle fiber types

Quiroz-Rothe

Rivero

2004

Microscopy Res & Technique

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“…Fibres of type IIB, fast-twitch glycolytic fibres, mainly perform glycolysis, and their metabolism contributes to the fast glycolytic rate in the early post mortem period (Choi et al 2013). Type IIA has a greater lipid and myoglobin content than type IIB (Essen-Gustavsson et al 1992). The morphological characteristics of doi: 10.17221/59/2015-CJAS muscle fibres are factors that influence energy metabolism in living and post mortem muscle ).…”

Section: Introductionmentioning

confidence: 99%

Relationship between muscle fibre characteristics and meat sensory properties in three nutria (Myocastor coypus) colour types

Tůmová¹,

Chodová²,

Uhlířová³

et al. 2016

CZECH J ANIM SCI

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AbstRAct:The study determined the muscle fibre characteristics and sensory properties of the longissimus lumborum muscle (LL) of eight-month-old nutrias of three colour types and the correlations between meat histochemical measurements and eating quality. The following nutria colour types were used in the experiment: Standard (ST), Moravian Silver (MS), and Prestice Multicolour (PM). The nutria colour type significantly (P < 0.001) affected the number of type IIB fibres with the lowest number in ST (108 per mm 2 ). The proportion of type I fibres varied between 10.6 and 14.2%; type IIA fibres varied between 6.1 and 7.8%; and type IIB fibres varied between 78.2 and 83.4% and it was not affected by colour type. The cross-sectional area (CSA) was 2.565-2.841 µm 2 in type I, 2.867-3.010 µm 2 in type IIA, and 4.698-5.517 µm 2 in type IIB fibres. The CSA of type IIB fibres (P < 0.001) was the largest in ST. The sensory trait of tenderness (P < 0.05) was the lowest in MS. Correlations between the proportion of fibres and sensory traits were not observed. The CSA of type I fibres correlated with flavour (0.19) and the CSA of type IIB fibres with tenderness (0.10).

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“…Essen-Gustavsson et al (1992) and Fiedler et al (1999) demonstrated that the fibre diameter in m. longissimus dorsi was larger in pigs susceptible to stress, when compared to that observed in stress-resistant animals. In turn, Gallant (1980) and Ackermann and Salomon (1991) did not observe such a relation.…”

Section: Effect Of Ryr1 Genotype On Microstructural Characteristics Omentioning

confidence: 96%

A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses

Kłosowska¹,

Kurył²,

Elminowska-Wenda³

et al. 2004

CZECH J ANIM SCI

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Muscle fibre formation takes place during embryonic development and is regulated by the MyoD gene family, which consists of four genes, MYOD1, myogenin, MYF5 and MRF4. A relationship was studied between MYOD1, myogenin and MYF5 genotypes and microstructural characteristics of the m. longissimus lumborum in pigs – crosses: Pietrain × (Polish Large White × Polish Landrace). The data included 115 unrelated animals slaughtered at about 105 kg live body weight. Within 45 min after exsanguination, samples were taken from the m. longissimus lumborum, frozen in liquid nitrogen and later analysed for the diameter of slow-twitch oxidative, fast-twitch oxidative and fast-twitch low-oxidative fibres, their proportion in a bundle, the proportion of pathological changes and number of fibres per unit area. The RYR1 and MyoD genotypes were determined using the PCR-RFLP technique. A significant or highly significant relation was observed between the diameter of all types of muscle fibres and genotype RYR1 – the highest values were recorded for homozygotes TT (genetically stress-sensitive). A relation between MyoD genotypes and microstructural characteristics of the m. longissimus lumborum was analysed on a group of 93 animals of the genotype CC or CT at locus RYR1. Sex appeared to have no significant effect on the muscle microstructural traits in this group of animals. The content of fast-twitch oxidative fibres (FTO) was significantly related to the MYF5 genotype, whereas that of fast-twitch low-oxidative fibres (FT) was affected by the MYOD1 and MYF5/DdeI genotypes. The proportion of angular fibres in a bundle was related to MYF5/HinfI genotype. The results showed that MyoD genes could be considered as candidate genes for some microstructural characteristics of m. longissimus lumborum in pigs.  

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Muscle fibre characteristics and metabolic response at slaughter in pigs of different halothane genotypes and their relation to meat quality

Cited by 80 publications

References 21 publications

Coordinated expression of myosin heavy chains, metabolic enzymes, and morphological features of porcine skeletal muscle fiber types

Coordinated expression of myosin heavy chains, metabolic enzymes, and morphological features of porcine skeletal muscle fiber types

Relationship between muscle fibre characteristics and meat sensory properties in three nutria (Myocastor coypus) colour types

A relationship between the PCR-RFLP polymorphism in porcine MYOG, MYOD1 and MYF5 genes and microstructural characteristics of m. longissimus lumborum in Pietrain × (Polish Large White × Polish Landrace) crosses

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