Mussel‐Inspired Cell‐Adhesion Peptide Modification for Enhanced Endothelialization of Decellularized Blood Vessels

Lee, Jung Seung; Lee, Kihong; Moon, Sung; Chung, Hyung Min; Lee, Jun Hyup; Um, Soong Ho; Kim, Dong Ik; Cho, Seung Woo

doi:10.1002/mabi.201400052

Cited by 47 publications

(32 citation statements)

References 42 publications

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“…Previous studies suggested that PDA can effectively promote NP–cell interactions on various polymer‐modified surfaces . The potential explanation for this increased NP–cell interactions ability was that the catechol groups of the compound can act as an immobilization site to bind with amino‐ or thiol‐functionalized biomolecules of the cell membrane or cell adhesive moieties in the extracellular matrix . Therefore, we can also assume that the catechol group of our AmQu may be the reason for the observed promoted NP–cell interactions and cellular internalization.…”

Section: Resultsmentioning

confidence: 99%

Quercetin‐Based Modified Porous Silicon Nanoparticles for Enhanced Inhibition of Doxorubicin‐Resistant Cancer Cells

Liu

Balasubramanian

Bhat

et al. 2016

Adv Healthcare Materials

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One of the most challenging obstacles in nanoparticle's surface modification is to achieve the concept that one ligand can accomplish multiple purposes. Upon such consideration, 3-aminopropoxy-linked quercetin (AmQu), a derivative of a natural flavonoid inspired by the structure of dopamine, is designed and subsequently used to modify the surface of thermally hydrocarbonized porous silicon (PSi) nanoparticles. This nanosystem inherits several advanced properties in a single carrier, including promoted anticancer efficiency, multiple drug resistance (MDR) reversing, stimuli-responsive drug release, drug release monitoring, and enhanced particle-cell interactions. The anticancer drug doxorubicin (DOX) is efficiently loaded into this nanosystem and released in a pH-dependent manner. AmQu also effectively quenches the fluorescence of the loaded DOX, thereby allowing the use of the nanosystem for monitoring the intracellular drug release. Furthermore, a synergistic effect with the presence of AmQu is observed in both normal MCF-7 and DOX-resistant MCF-7 breast cancer cells. Due to the similar structure as dopamine, AmQu may facilitate both the interaction and internalization of PSi into the cells. Overall, this PSi-based platform exhibits remarkable superiority in both multifunctionality and anticancer efficiency, making this nanovector a promising system for anti-MDR cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Quercetin‐Based Modified Porous Silicon Nanoparticles for Enhanced Inhibition of Doxorubicin‐Resistant Cancer Cells

Liu

Balasubramanian

Bhat

et al. 2016

Adv Healthcare Materials

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“…Besides the RGDS motif, other adhesive ligands such as the laminin derived YIGSR (Tyr-Ile-Gly-Ser-Arg) sequence [22,23] have been shown to promote EC adhesion and migration without enhancing platelet adhesion [24]. For instance, this peptide has enhanced the adhesion of ECs in hydrogels [13,25], PET [26], polyurethane [27,28], poly(2-hydroxyethyl methacrylate) [29] and decellularized scaffolds [30]. Finally, another cell adhesive sequence found in fibronectin, the REDV (Arg-Glu-Asp-Val) peptide, which targets ECs via the integrin ␣4␤1, has been reported to selectively promote EC adhesion and spreading over SMCs and platelets [22,31].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferation

Castellanos

Mas‐Moruno

Grau

et al. 2017

Applied Surface Science

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Biomimetic surface modification with peptides that have specific cell-binding moieties is a promising approach to improve endothelialization of metal-based stents. In this study, we functionalized CoCr surfaces with RGDS, REDV, YIGSR peptides and their combinations to promote endothelial cells (ECs) adhesion and proliferation. An extensive characterization of the functionalized surfaces was performed by XPS analysis, surface charge and quartz crystal microbalance with dissipation monitoring (QCM-D), which demonstrated the successful immobilization of the peptides to the surface. Cell studies demonstrated that the covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represents the most powerful strategy to enhance the early stages of ECs adhesion and proliferation, indicating a positive synergistic effect between the two peptide motifs. Although these peptide sequences slightly increased smooth muscle cells (SMCs) adhesion, these values were ten times lower than those observed for ECs. The combination of RGDS with the REDV sequence did not show synergistic effects in promoting the adhesion or proliferation of ECs. The strategy presented in this study holds great potential to overcome clinical limitations of current metal stents by enhancing their capacity to support surface endothelialization.Peer ReviewedPostprint (author's final draft

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“…Thus, there has been a corresponding increase in the study of decellularized tissue modification to enhance endothelialization and reduce thrombogenicity. In attempts to accelerate endothelialization, researchers have coated decellularized grafts with VEGF, VEGF hydrogels, integrin ligands, and cell adhesion peptides [19–23]. Other groups have chosen to focus on decreasing graft thrombogenicity.…”

Section: Introductionmentioning

confidence: 99%

Improving in vivo outcomes of decellularized vascular grafts via incorporation of a novel extracellular matrix

et al. 2017

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Each year, hundreds of thousands coronary bypass procedures are performed in the US, yet there currently exists no off-the-shelf alternative to autologous vessel transplant. In the present study, we investigated the use of mouse thrombospondin-2 knockout (TSP2 KO) cells, which secrete a non-thrombogenic and pro-migratory extracellular matrix (TSP2 KO ECM), to modify small diameter vascular grafts. To accomplish this, we first optimized the incorporation of TSP2 KO ECM on decellularized rat aortas. Because MMP levels are known to be elevated in TSP2 KO cell culture, it was necessary to probe the effect of the modification process on the graft's mechanical properties. However, no differences were found in suture retention, Young's modulus, or ultimate tensile strength between modified and unmodified grafts. Platelet studies were then performed to determine the time point at which the TSP2 KO ECM sufficiently reduced thrombogenicity. Finally, grafts modified by either TSP2 KO or WT cells or unmodified grafts, were implanted in an abdominal aortic interposition model in rats. After 4 weeks, grafts with incorporated TSP2 KO ECM showed improved endothelial and mural cell recruitment, and a decreased failure rate compared to control grafts. Therefore, our studies show that TSP2 KO ECM could enable the production of off-the-shelf vascular grafts while promoting reconstruction of native vessels.

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Mussel‐Inspired Cell‐Adhesion Peptide Modification for Enhanced Endothelialization of Decellularized Blood Vessels

Cited by 47 publications

References 42 publications

Quercetin‐Based Modified Porous Silicon Nanoparticles for Enhanced Inhibition of Doxorubicin‐Resistant Cancer Cells

Quercetin‐Based Modified Porous Silicon Nanoparticles for Enhanced Inhibition of Doxorubicin‐Resistant Cancer Cells

Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferation

Improving in vivo outcomes of decellularized vascular grafts via incorporation of a novel extracellular matrix

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