Mussel-Inspired, Injectable Polyurethane Tissue Adhesives Demonstrate In Situ Gel Formation under Mild Conditions

Abstract

Tissue adhesives are widely desired in surgical wound closure and interfacial modification of medical devices. However, poor adhesive strength, low mechanical compliance, high costs, and biotoxicity of traditional adhesives limit their biomedical applications. In this work, we fabricated a class of mussel-inspired polyurethane adhesives (MPUAs) with robust properties, including in situ gel formation under mild conditions, accommodation of intricate wounds, strong adhesion, and good biocompatibility. Dopamine-modified lysine (LDA), as a chain extender, was incorporated into linear polyurethane as a crucial adhesive unit, and biologically sourced lysine was used as a mild curing agent for in situ gel. The premixed polyurethane adhesives can be fluently injected and can fill irregular and complicated defects. After mixing for several minutes, the adhesives can strongly bond multiple substrates, such as metallic materials, organic materials, and inorganic nonmetallic materials. In particular, they can strongly cohere the fresh (wet) biological tissues with a ductile interface, and the lap shear strength (24.5 ± 2.0 kPa) of the MPUAs was six times higher than that of the commercial fibrin glue. Moreover, the bursting pressure of MPUAs on the porcine aorta was 108.2 ± 3.8 mmHg, which can satisfy most of the surgical requirements (≤20 mmHg). Given its good biocompatibility, this system would provide potential applications in clinical surgery and biological coatings of medical devices.

A bioinspired Janus polyurethane membrane for potential periodontal tissue regeneration

“…It was found that the N + content on the smooth surface of the polyurethane membrane (20.19%) was much more than that on the rough surface (15.12%), while the CO content was lower. These results indicated that the smooth surface had more quaternary ammonium salt groups and the rough surface had more dopamine groups (phenol hydroxyl oxidizes to quinone 42 ). This spontaneous microphase separation depends on the different migration of functional segments between the Gemini QAS and LDA.…”

“…These results indicated that the smooth surface had more quaternary ammonium salt groups and the rough surface had more dopamine groups (phenol hydroxyl oxidizes to quinone 42 ). This spontaneous microphase separation depends on the different migration of functional segments between the Gemini QAS and LDA.…”

“…The lysine-derivative Gemini quaternary ammonium salt (EG12) and lysine-dopamine (LDA) as the chain extenders were synthesized in our laboratory according to our previous method. 39,42 Then the polyurethane was synthesized using PEG, PCL, LDI, BDO, EG12 and LDA. The synthetic procedure of polyurethane is as follows.…”

Using extracellular matrix as the bio-glue for wound repair in the surgery

“…What's more, they are non-toxic in vitro and in vivo (Stakleff et al, 2013). Zhao et al (Zhao et al, 2021) prepared a rapid in situ gelling of mussel polyurethane adhesives (MPUAs) under mild conditions, which showed good biocompatibility and strong adhesion to complex wounds. Faxing Zou et al (Zou et al, 2022) prepared a novel tissue adhesive composed of L-arginine, degradable polyurethane (DLPU) and GelMA for wound repair.…”

“…Biomedical materials are used in trauma approaches to replace or restore the anatomic structure and function of damaged or missing tissues following any injury or disease by combining the topographical cues of biomaterials with cells or bioactive molecules (Tan et al, 2021). Bio-glue intended for trauma repair are often derived from particular extracellular matrix (ECM) molecules [e.g., fibrin (Kalsi et al, 2017), collagen (Schiele et al, 1992), hyaluronic acid (Luo et al, 2020)], or other natural polymers [e.g., gelatin/alginate (Song et al, 2022), chitosan (Lu et al, 2018)] or synthetic polymers [e.g., cyanoacrylate (Carvalho et al, 2021), polyurethane (Zhao et al, 2021), polyethylene glycol (Privett et al, 2021)]. While both natural and synthetic polymers are widely utilized as tissue adhesives, the use of ECM bio-glue is rarely seen (Nishiguchi and Taguchi, 2021;Yazdanpanah et al, 2022).…”