Mussel-inspired surface modification of magnetic@graphite nanosheets composite for efficient Candida rugosa lipase immobilization

Abstract

By the facile adhesion way, the novel composite complex by polydopamine (PDA) and magnetic graphite nanosheets (Fe3O4@GNSs) has been successfully synthesized. The resulting composite was characterized by means of scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectra, and Raman spectra, X-ray diffraction, X-ray photoelectron spectroscopy, and vibrating sample magnetometry. Meanwhile, the PDA functionalized Fe3O4@GNSs (Fe3O4@GNSs-PDA) was applied for Candida rugosa lipase (CRL) immobilization covalently without any toxic coupling agent. Combining the superior physical properties and chemical stability of Fe3O4@GNSs and the well biocompatibility, functional characteristics of PDA, the Fe3O4@GNSs-PDA composite displayed several advantages, including the high enzyme capacity, enzyme activity and stability and a decrease in enzyme loss. Our work demonstrated that the mussel-inspired Fe3O4@GNSs can be extended to many other applications such as biocatalytic, genetic and industrial.

Facile and Rapid Synthesis of Hollow Magnetic Mesoporous Polydopamine Nanoflowers with Tunable Pore Structures for Lipase Immobilization: Green Production of Biodiesel

“…However, the catalytic activity of CRL@HM-MPDA-NFs reaches its maximum when the lipase concentration is 2.0 mg/mL. Concentrations that are too low or too high will result in a decrease in catalytic activity, which were similar to the previous reports . As can be seen from Figure S5B, the immobilization efficiency constantly increased and eventually tended to be stable with the prolongation of time.…”

“…Concentrations that are too low or too high will result in a decrease in catalytic activity, which were similar to the previous reports. 43 As can be seen from Figure S5B, the immobilization efficiency constantly increased and eventually tended to be stable with the prolongation of time. The catalytic activity of CRL@HM-MPDA-NFs reaches its maximum when the immobilization time is 3 h. Once the immobilization time exceeded 3 h, the catalytic activity of CRL@HM-MPDA-NFs begins to decline, mainly due to the excessive coupling between the lipase biomolecules.…”

Enhanced Performance of Magnetic Graphene Oxide-Immobilized Laccase and Its Application for the Decolorization of Dyes

“…The magnetic response property, large surface area, two-dimensional structure, easy surface modification, large enzyme immobilization capacity, simple preparation, and satisfactory reusability of magnetic GO (MGO) has received considerable attention in enzyme immobilization and related research [ 14 , 15 ]. MGO and graphene-based magnetic nanocomposites were synthesized as enzyme immobilization support for catalase, glucoamylase, lipase, and β-galactosidase [ 15 , 16 , 17 , 18 , 19 ]. The synthesized immobilized enzyme showed high activity recovery, easy recycling, improved catalytic activity, and stability compared to the free enzyme.…”

Enzyme Engineering for In Situ Immobilization

“…Other common carrier materials include carboxymethyl-cellulose, starch, collagen, agarose, modified sepharose, ion exchange resins, active charcoal, clay, aluminium oxide, titanium, diatomaceous earth, hydroxyapatite, ceramic, celite, or treated porous glass as well as various polymers [25,26]. Materials can be combined to assemble into hierarchical composite structures the properties of which can be fine-tuned towards the targeted reaction conditions [27,28,29].…”