1990
DOI: 10.1016/0022-2836(90)90356-q
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Mutagenesis by proximity to the recA gene of Escherichia coli

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Cited by 9 publications
(13 citation statements)
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“…The recA+ strain was EST2422 (XrecA + cl ind) (27). The media, M9-CAA (a Casamino-Acids-supplemented M9), LB broth, and SFLB (a salt-free plating agar based on LB broth), have been previously described (26,27). The antibiotics used were kanamycin (30 ,ug/ml) and rifampin (25 pug/ml).…”
Section: Methodsmentioning
confidence: 99%
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“…The recA+ strain was EST2422 (XrecA + cl ind) (27). The media, M9-CAA (a Casamino-Acids-supplemented M9), LB broth, and SFLB (a salt-free plating agar based on LB broth), have been previously described (26,27). The antibiotics used were kanamycin (30 ,ug/ml) and rifampin (25 pug/ml).…”
Section: Methodsmentioning
confidence: 99%
“…Strains with unmutated recA1202 alleles produce dark blue (DB) colonies on X-Gal plates because the high coprotease activity derepresses the dinD gene and the fused lacZ gene (47). However, in combination with the pSE117 plasmid, the recA1202 gene exhibits a very high frequency of mutation in the recA gene, which results in decreased RecA coprotease activity and pale blue (PB) colonies (26). Thus, after incubation at 32°C for 24 h, there were many transformants with stable PB or blue (B) colors that could be picked and purified.…”
Section: Methodsmentioning
confidence: 99%
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