Mutagenesis in cultured mammalian cells

Human Adenovirus Type 2 but Not Adenovirus Type 12 Is Mutagenic at the Hypoxanthine Phosphoribosyltransferase Locus of Cloned Rat Liver Epithelial Cells

“…For mutation experiments, C3 cells were infected with a range of MOIs, and various expression times were used because of the reported importance of the relationship between cell killing, expression time, and the isolation of induced mutants (18).…”

The influence of heterogeneity of somatic mammalian cell system on mutagenesis manifestation induced by transforming genes of adenovims

“…Поэтому считаем целесообразным аппроксимировать первое проявление мутагенеза убывающей экспонентой. Подтверждением этого вывода являются данные работы [49]: при исследовании других генных локусов и мутагенов максимальный мутагенный эффект обнаруживался иногда сразу с t = 0 и убывал по экспоненте.…”

The isolation and initial characterization of dibucaine-resistant variants of a mouse lymphocytic cell line

“…Because ICR 191 may cause frameshift or deletion mutations in animal cells, Friedrich and Coffino (1977) doses that result in a 70% to 90% cell lethality (Thompson, 1979;Basilico, 1977). Because variability is observed in the percentage of cell survival induced with the same mutagen dose, it is important to determine the lethal effect of a particular mutagen dose in each experi mental trial (Shapiro and Varshaver, 1977;Cole and Arlett, 1976). In addition, because different cell lines and different cell strains derived from the same cell line are known to exhibit different sensitivities to mutagen treatment, a dose response for a particular mutagen must be established for each cell type (Thompson and Baker, 1973).…”

“…Mutation frequency is distinguished from mutation rate, which defines the average number of mutations to a particular mutant phenotype per cell per generation as determined by fluctuation analysis (Thompson and Baker, 1973;Baker et al, 1974;, Shapiro and Varshaver, 1977). be increased by mutagen treatment (Chu and Mailing, 1968;Siminovitch, 1976;Till et al, 1973) There is evidence, lending sTç>port to the former hypothesis, that suggests that procaine and benzocaine may be acting at sites different from tetracaine and dibucaiine.…”