Mutagenesis in olive (<i>Olea europaea</i>L.) calli caused by sodium azide and detection of mutants using ISSR and RAPD markers

Sheikh, Amin Alborzian Deh; Moradnejad, M

doi:10.1080/14620316.2014.11513062

Cited by 9 publications

(3 citation statements)

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“…Several molecular markers have been used to characterize the genetic variation induced among them, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and nucleotide polymorphism unique (SNP) [25]. RAPD markers have been used to assess the genetic variability induced by physical and chemical mutagens in several cultivable species [44,[58][59][60][61][62].…”

Section: Ems and Plant Omicsmentioning

confidence: 99%

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila¹,

Gutiérrez-Miceli²

2020

Phyton

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Ethyl methanesulfonate is a chemical mutagen, which is currently being used in plant breeding, to increase genetic variability in genes of agronomic interest, of species useful in agriculture. It primarily causes single base point mutations by inducing guanine alkylation, resulting in GC to AT transitions. Its effect is different between clones of a genotype and between genotypes of the same species. This review presents the results obtained in recent research, where its effect on plant tissues, callus, and cells in suspension has been evaluated. Changes in the phenotypic expression of somaclonal variants were reported, involving morphology, production of secondary metabolites, changes in metabolic routes of resistance, tolerance to stress, increased seed yield, among others. In addition, this review compiles the doses and guidelines to consider before using this mutagen, which can serve as a guide for future trials in deciding the response variables, the type of plant explants and the selection of the study model. Mutant lines have allowed plant breeders to have a collection of plants with different characteristics, in places where the cultivar does not have its center of origin. It is important to note that it is still necessary to continue evaluating the heritability of mutations and their behaviour in the environment where they will be established, in order to obtain new varieties of plants that can be cultivated with uniformity in their genetic response.

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Section: Ems and Plant Omicsmentioning

confidence: 99%

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Joya-Dávila¹,

Gutiérrez-Miceli²

2020

Phyton

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“…In addition, DNA markers can also be used to study the effect of the applied compounds on a molecular basis [ 28 ]. ISSR molecular markers were used to determine the molecular changes due to the application of the proline or glycine betaine on Cicer arietinum [ 29 ] and chemical mutagenesis by NaN 3 in Olea europaea l [ 30 ]. Furthermore, gene expression is affected by nano-fertilizers.…”

Section: Introductionmentioning

confidence: 99%

The influence of potassium nanoparticles as a foliar fertilizer on onion growth, production, chemical content, and DNA fingerprint

Salama,

Khater,

Abd El-Aziz

2024

Heliyon

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“…Since Plumbago indica L. is a heterostylous plant and has not been seen to bear fruit in nature, it is difficult to improve its cold hardiness by cross breeding. Integrated tissue culture and chemical mutagenesis for mutant induction provides a new breeding technique for creating new cold-tolerant mutant materials that can shorten breeding cycles and accelerate breeding programs ( Shi et al., 2022 ) and has been successfully applied to several plants, including rice ( Chen et al., 2013 ), Cerastigma willmottianum Stapf ( Shi et al., 2022 ), olive ( Sheikh and Moradnejad, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

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Plumbago indica L. is a perennial herb with ornamental and anticancer medicinal functions widely distributed in the tropics. It is affected by temperature and cannot bloom normally in colder subtropical regions, which seriously affects its ornamental value. To create low-temperature resistance mutants and enrich new germplasm resources, this study used tissue culture and chemical reagent (0.5 mmol/L NaN3) and low-temperature stress (0°C, full darkness for 48h) induction to target and screen for cold-resistance mutants. The results showed that the ISSR band polymorphism ratio of the 24 suspected mutant materials was 87.5%. The DNA profiles of the 9 mutants initially identified were altered. The content of plumbagin in the stems and leaves of the mutants was examined, and it was found that the accumulation in the leaves of the mutant SA24 could be as high as 3.84 times that of the control, which was 0.5991%. There were significant differences in the anatomical structures of roots, stems and leaves. The mutants mostly exhibited reduced root diameter (only 0.17-0.69 times that of CK), increased stem diameter (up to 2.19 times that of CK), enlarged mesophyll cells, increased thickness (up to 1.83 times that of CK) and high specificity, which are thought to be important for the different cold resistance obtained by the mutants. In the cold resistance experiment, four cold-tolerant mutants were successfully screened according to their morphological characteristics and physiological indexes, and the mutagenesis efficiency could be as high as 2.22% and did not affect the accumulation of plumbagin in their stems and leaves, even higher than CK. The responses of the screened mutants SA15, SA19, SA23 and SA24 to low temperature showed slower leaf wilting, higher light energy conversion efficiency, less accumulation of MDA content, increased enzymatic activities of antioxidant enzymes (SOD, CAT, POD) and more accumulation of soluble sugars and proline content. These characteristics are consistent with the response of cold-resistance plants to low temperatures. The cold- resistance mutants cultivated in soil were observed of agronomic and ornamental traits for one year, mainly manifested as delayed flowering and delayed entry into the senescence stage. This study provides a more rapid and accurate technique for identifying and screening cold-tolerant mutants, and lays the foundation for future experiments on the creation of new cold-resistant varieties.

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Mutagenesis in olive (Olea europaeaL.) calli caused by sodium azide and detection of mutants using ISSR and RAPD markers

Cited by 9 publications

References 0 publications

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

Ethyl Methanesulfonate as Inductor of Somaclonal Variants in Different Crops

The influence of potassium nanoparticles as a foliar fertilizer on onion growth, production, chemical content, and DNA fingerprint

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

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